Research on cloud manufacturing platform for small and medium-sized enterprises

With the adjustment of China’s economic structure, it is essential for manufacturing enterprises, especially SMEs, to improve their core competitiveness. Based on the service mode of Cloud manufacturing, this paper designs and develops a cloud manufacturing platform for SMEs. Firstly, according to the characteristics of cloud manufacturing, the overall framework of the platform is determined, and the corresponding function tree is refined. Then, the key technologies are studied, including the modeling and standardization of manufacturing services, the matching of task, the execution, the online monitoring of service, and the evaluation of post-service. The functions of service access, service matching, outsourcing task management, remote monitoring, and evaluation of tasks are realized. Finally, the platform is applied in the mold manufacturing industry, and an example analysis is carried out to verify the effectiveness of the proposed platform

Research on cloud manufacturing platform for small and medium-sized enterprises

With the adjustment of China’s economic structure, it is essential for manufacturing enterprises, especially SMEs, to improve their core competitiveness. Based on the service mode of Cloud manufacturing, this paper designs and develops a cloud manufacturing platform for SMEs. Firstly, according to the characteristics of cloud manufacturing, the overall framework of the platform is determined, and the corresponding function tree is refined. Then, the key technologies are studied, including the modeling and standardization of manufacturing services, the matching of task, the execution, the online monitoring of service, and the evaluation of post-service. The functions of service access, service matching, outsourcing task management, remote monitoring, and evaluation of tasks are realized. Finally, the platform is applied in the mold manufacturing industry, and an example analysis is carried out to verify the effectiveness of the proposed platform

The application of HER2 and CD47 CAR-macrophage in ovarian cancer

Abstract Background The chimeric antigen receptor (CAR)-T therapy has a limited therapeutic effect on solid tumors owing to the limited CAR-T cell infiltration into solid tumors and the inactivation of CAR-T cells by the immunosuppressive tumor microenvironment. Macrophage is an important component of the innate and adaptive immunity, and its unique phagocytic function has been explored to construct CAR macrophages (CAR-Ms) against solid tumors. This study aimed to investigate the therapeutic application of CAR-Ms in ovarian cancer. Methods In this study, we constructed novel CAR structures, which consisted of humanized anti-HER2 or CD47 scFv, CD8 hinge region and transmembrane domains, as well as the 4-1BB and CD3ζ intracellular domains. We examined the phagocytosis of HER2 CAR-M and CD47 CAR-M on ovarian cancer cells and the promotion of adaptive immunity. Two syngeneic tumor models were used to estimate the in vivo antitumor activity of HER2 CAR-M and CD47 CAR-M. Results We constructed CAR-Ms targeting HER2 and CD47 and verified their phagocytic ability to ovarian cancer cells in vivo and in vitro. The constructed CAR-Ms showed antigen-specific phagocytosis of ovarian cancer cells in vitro and could activate CD8+ cytotoxic T lymphocyte (CTL) to secrete various anti-tumor factors. For the in vivo model, mice with human-like immune systems were used. We found that CAR-Ms enhanced CD8+ T cell activation, affected tumor-associated macrophage (TAM) phenotype, and led to tumor regression. Conclusions We demonstrated the inhibition effect of our constructed novel HER2 CAR-M and CD47 CAR-M on target antigen-positive ovarian cancer in vitro and in vivo, and preliminarily verified that this inhibitory effect is due to phagocytosis, promotion of adaptive immunity and effect on tumor microenvironment