14 research outputs found
Differential risk factor profile and neuroimaging markers of small vessel disease between lacunar ischemic stroke and deep intracerebral hemorrhage
An Induced Hypersensitive-Like Response Limits Expression of Foreign Peptides via a Recombinant TMV-Based Vector in a Susceptible Tobacco
BACKGROUND: By using tobacco mosaic virus (TMV)-based vectors, foreign epitopes of the VP1 protein from food-and-month disease virus (FMDV) could be fused near to the C-terminus of the TMV coat protein (CP) and expressed at high levels in susceptible tobacco plants. Previously, we have shown that the recombinant TMV vaccines displaying FMDV VP1 epitopes could generate protection in guinea pigs and swine against the FMDV challenge. Recently, some recombinant TMV, such as TMVFN20 that contains an epitope FN20 from the FMDV VP1, were found to induce local necrotic lesions (LNL) on the inoculated leaves of a susceptible tobacco, Nicotiana tabacum Samsun nn. This hypersensitive-like response (HLR) blocked amplification of recombinant TMVFN20 in tobacco and limited the utility of recombinant TMV vaccines against FMDV. METHODOLOGY/PRINCIPAL FINDINGS: Here we investigate the molecular mechanism of the HLR in the susceptible Samsun nn. Histochemical staining analyses show that these LNL are similar to those induced in a resistant tobacco Samsun NN inoculated with wild type (wt) TMV. The recombinant CP subunits are specifically related to the HLR. Interestingly, this HLR in Samsun nn (lacking the N/N'-gene) was able to be induced by the recombinant TMV at both 25°C and 33°C, whereas the hypersensitive response (HR) in the resistant tobacco plants induced by wt TMV through the N/N'-gene pathways only at a permissive temperature (below 30°C). Furthermore, we reported for the first time that some of defense response (DR)-related genes in tobacco were transcriptionally upregulated during HLR. CONCLUSIONS: Unlike HR, HLR is induced in the susceptible tobacco through N/N'-gene independent pathways. Induction of the HLR is associated with the expression of the recombinant CP subunits and upregulation of the DR-related genes
Spatial Distribution Characteristics and Influencing Factors of Pro-Poor Tourism Villages in China
This paper aims to contribute to the effectiveness of pro-poor tourism in rural areas. We use 5770 pro-poor tourism villages in China as the research objects; the spatial distribution characteristics of pro-poor tourism villages in China are analyzed using a combination of disequilibrium index, kernel density analysis, and spatial autocorrelation; their influencing factors are detected using a geographical detector and overlay analysis. The study results show the following: (1) The distribution of pro-poor tourism villages is exceptionally uneven in three zones, eight regions, and inter-provincial levels, forming a high-density cluster belt that includes Hebei, Henan, Anhui, and Hubei, and five high-density cluster cores that include southern Gansu, Sichuan–Gansu–Shaanxi border area; Guizhou, Hunan, and Chongqing border area; southern Sichuan; and southwest Guizhou. (2) Regarding spatial correlations, the pro-poor tourism villages in central and western regions are in hot spots, while those in eastern regions are in cold spots. The hot spots gradually increase, while cold spots gradually decrease, and the clustering trend of the distribution of the pro-poor tourism villages is increasingly apparent. (3) Pro-poor tourism villages are affected by social, economic, industrial, and other human factors as well as natural geographical factors such as terrain, precipitation, river, and climate, among which the industrial factors have a more significant impact. Pro-poor tourism villages are concentrated in humid mountainous areas with an altitude of about 1000 m and an annual precipitation of more than 800 mm, and they are mostly distributed in the subtropical monsoon climate zone closer to the river and more suitable climate. (4) It is suggested that pro-poor tourism villages can be divided into four types: the resource underutilization type, mountain environment restriction type, traffic location non-optimization type, and industrial development lagging type, and the sustainable development strategies of different types of pro-poor tourism villages are proposed
Spatial Distribution Characteristics and Influencing Factors of Pro-Poor Tourism Villages in China
This paper aims to contribute to the effectiveness of pro-poor tourism in rural areas. We use 5770 pro-poor tourism villages in China as the research objects; the spatial distribution characteristics of pro-poor tourism villages in China are analyzed using a combination of disequilibrium index, kernel density analysis, and spatial autocorrelation; their influencing factors are detected using a geographical detector and overlay analysis. The study results show the following: (1) The distribution of pro-poor tourism villages is exceptionally uneven in three zones, eight regions, and inter-provincial levels, forming a high-density cluster belt that includes Hebei, Henan, Anhui, and Hubei, and five high-density cluster cores that include southern Gansu, SichuanâGansuâShaanxi border area; Guizhou, Hunan, and Chongqing border area; southern Sichuan; and southwest Guizhou. (2) Regarding spatial correlations, the pro-poor tourism villages in central and western regions are in hot spots, while those in eastern regions are in cold spots. The hot spots gradually increase, while cold spots gradually decrease, and the clustering trend of the distribution of the pro-poor tourism villages is increasingly apparent. (3) Pro-poor tourism villages are affected by social, economic, industrial, and other human factors as well as natural geographical factors such as terrain, precipitation, river, and climate, among which the industrial factors have a more significant impact. Pro-poor tourism villages are concentrated in humid mountainous areas with an altitude of about 1000 m and an annual precipitation of more than 800 mm, and they are mostly distributed in the subtropical monsoon climate zone closer to the river and more suitable climate. (4) It is suggested that pro-poor tourism villages can be divided into four types: the resource underutilization type, mountain environment restriction type, traffic location non-optimization type, and industrial development lagging type, and the sustainable development strategies of different types of pro-poor tourism villages are proposed
TMV recombinants encoding fused foreign transmembrane domains to the CP subunit caused local necrotic response on susceptible tobacco
AbstractWith regard to the effects of various foreign peptides fused to the coat protein subunits on the infectivity of corresponding TMV recombinants, some of TMV recombinants were found to induce necrotic local lesions on the inoculated leaves of susceptible tobacco. This paper reported that there existed a group of TMV recombinants in which the fused foreign peptides contained a transmembrane domain according to the predictions by three programs of SOSUI, TMpred and DAS. Further studies showed for the first time that a foreign transmembrane domain in a fused peptide of the corresponding TMV recombinant would result in the local lesions on the susceptible tobacco leaves. In addition, it was concluded that none of the TMV recombinants that systematically infected susceptible tobacco contained a transmembrane domain in the coat protein subunits