Unveiling molecular mechanisms of pepper resistance to Phytophthora capsici through grafting using iTRAQ-based proteomic analysis

Abstract Phytophthora blight severely threatens global pepper production. Grafting bolsters plant disease resistance, but the underlying molecular mechanisms remain unclear. In this study, we used P. capsici-resistant strain ‘ZCM334’ and susceptible strain ‘Early Calwonder’ for grafting. Compared to self-rooted ‘Early Calwonder’ plants, ‘ZCM334’ grafts exhibited delayed disease onset, elevated resistance, and reduced leaf cell damage, showcasing the potential of grafting in enhancing pepper resistance to P. capsici. Proteomic analysis via the iTRAQ technology unveiled 478 and 349 differentially expressed proteins (DEPs) in the leaves and roots, respectively, between the grafts and self-rooted plants. These DEPs were linked to metabolism and cellular processes, stimulus responses, and catalytic activity and were significantly enriched in the biosynthesis of secondary metabolites, carbon fixation in photosynthetic organizations, and pyruvate metabolism pathways. Twelve DEPs exhibiting consistent expression trends in both leaves and roots, including seven related to P. capsici resistance, were screened. qRT-PCR analysis confirmed a significant correlation between the protein and transcript levels of DEPs after P. capsici inoculation. This study highlights the molecular mechanisms whereby grafting enhances pepper resistance to Phytophthora blight. Identification of key genes provides a foundation for studying the regulatory network governing the resistance of pepper to P. capsici

Increasing Snow–Soil Interface Temperature in Farmland of Northeast China from 1979 to 2018

The presence of seasonal snow cover in the cold season can significantly affect the thermal conditions of the ground. Understanding the change of the snow–soil interface temperature (TSS) and its environmental impact factors is essential for predicting subnivean species changes and carbon balance in future climatic conditions. An improved Snow Thermal Model (SNTHERM) is employed to quantify TSS in farmland of Northeast China (NEC) in a 39-year period (1979–2018) firstly. This study also explored the variation tendency of TSS and its main influencing factors on grid scale. The result shows that annual average TSS and the difference between TSS and air temperature (TDSSA) increased rapidly between 1979 and 2018 in the farmland of NEC, and we used the Mann–Kendall test to further verify the increasing trends of TSS and TDSSA on aggregated farmland of NEC. The correlation analysis showed that mean snow depth (MSD) is the most pivotal control factor in 95% of pixels and TDSSA increases as MSD increases. Snow depth can better predict the change of TSS in deep–snow regions than average winter temperature (TSA). The results of this study are of great significance for understanding the impact of snow cover on the energy exchange between the ground and the atmosphere in the cold climate

An investigation on microwave transmissivity at frequencies of 18.7 and 36.5 GHz for diverse forest types during snow season

Forests have invariably been considered as an obstacle in retrieving land surface parameters from spaceborne passive microwave brightness temperature (TB) observations. For quantifying the effect of forests on microwave signals, several models have been developed. However, these models rarely reveal the dependence of microwave radiation on forest types, which can hardly meet the needs of high-accuracy retrieval of terrestrial parameters in forested regions. A ground-based microwave radiometric observation experiment was designed to investigate the dependence of microwave radiation on frequency, polarization, and forest type. Downward TB at 18.7- and 36.5-GHz for horizontal- and vertical-polarization from the forest canopy was measured at 14 sample plots in Northeast China, along with snowpack and forest structural parameters. By providing fits to experimental data, new empirical transmissivity models for three forest types were developed, as a function of woody stem volume and depending on the frequency/polarization. The proposed models give diverse asymptotic transmissivity saturation levels and the corresponding saturation point of woody stem volume for different forest types. Root-mean-square error results between TB simulations and Advanced Microwave Scanning Radiometer-2 observations are approximately 3–6 K. This study provides an experimental and theoretical reference for further development of inversion models for snow parameters in forested areas

Endoscopic Submucosal Tunnel Dissection for Large Gastric Neoplastic Lesions: A Case-Matched Controlled Study

Aim. To evaluate the efficacy and safety of endoscopic submucosal tunnel dissection (ESTD) for resection of large superficial gastric lesions (SGLs). Methods. The clinicopathological records of patients performed with ESTD or endoscopic submucosal dissection (ESD) for SGLs between January 2012 and January 2014 were retrospectively reviewed. 7 cases undergoing ESTD were enrolled to form the ESTD group. The cases were individually matched at a 1 : 1 ratio to other patients performed with ESD according to lesion location, ulcer or scar findings, resected specimen area, operation time and operators, and the matched cases constituting the ESD group. The treatment outcomes were compared between the two groups. Results. The mean specimen size was 46 mm. 10 lesions were located in the cardia and 4 lesions in the lesser curvature of the lower gastric body. En bloc resection was achieved for all lesions. The mean ESTD resection time was 69 minutes as against 87.7 minutes for the ESD (P=0.01). The mean resection speed was faster for ESTD than for ESD (18.86 mm2/min versus 13.76 mm2/min, P=0.03). There were no significant differences regarding the safety and curability during the endoscopic follow-up (mean 27 months). Conclusions. ESTD is effective and safe for the removal of SGLs and appears to be an optimal option for patients with large SGLs at suitable sites

Folium crataegi boosts skin regeneration for burn injury in rats through multiple ways

Appropriate topical dressings for burn treatments are important to accelerate skin wound recovery and prevent external infections. This study aimed to evaluate the effect and investigate the mechanism of folium crataegi (Crataegus pinnatifida Bge.) for the treatment of burn wounds, as well as to compare the therapeutic effects of aqueous extracts (HLW) and alcoholic extracts (HLE) from folium crataegi. The results demonstrated that both HLW and HLE groups exhibited a higher wound contraction rate than the silver sulfadiazine (SSD) ointment group. Moreover, HLW showed more significant wound repair effects than HLE. HLW significantly increased levels of EGF and FGF-2 in wound tissue, as well as TGF-β1, VEGF, CAT and IL-10 in serum. Folium crataegi extract, especially aqueous extracts, exerted good anti-inflammatory, anti-oxidant and anti-bacterial effects by upregulating the expression of lag3, txn1 and slpi, respectively. Folium crataegi extract significantly inhibits the expression of npas2, a key gene in the circadian rhythm pathway. In conclusion, this research illustrated that the folium crataegi extract, especially aqueous extracts, had better therapeutic effects on skin burns through multiple ways, possibly including a novel mechanism related to circadian rhythm pathway. These findings suggest that folium crataegi could be a valuable source of compounds for enhancing skin regeneration through multiple ways

Biomimetic honeycomb Zn anode enabled multi-field regulation toward highly stable flexible Zn-ion batteries

Flexible Zn-ion batteries (ZIBs) emerge as a promising entrant for flexible and safe energy systems in the post-Li era, while the instability of Zn anode including inferior flexibility, uncontrollable plating, and dendrite growth remains a challenge. Naturally inspired, a topology-optimized biomimetic honeycomb Zn (BH-Zn) anode through mechanical-electrochemical processing is demonstrated. Numerical simulations and experimental observations reveal the BH-Zn engenders smooth current–stress–thermal field distributions, concurrently realizing the multi-field regulation effect and boosted stability. After in situ alloying, the BH-Zn enables half-diminished voltage polarization, superior electrochemical stability of 2000 h cycling, and thermal stability even at 30 mA cm−2. Moreover, the assembled ZIBs manifest over 20 times enhanced capacity retention and are integrated as a self-powered wearable system for real-time health monitoring. This strategy can be extended to customizable metal anodes and promises to be applied in stable flexible batteries.The authors acknowledge the financial support from the National Natural Science Foundation of China (grant nos. 52175534, U22A20193), the Natural Science Foundation of Hunan Province, China (grant no. 2022JJ30154), the Science and Technology Innovation Program of Hunan Province (grant no. 2021RC3052), the Fundamental Research Funds for the Central Universities (grant no. 531118010016), and the State Key Laboratory of Advanced Design and Manufacturing for Vehicle Body (grant no. 72175008). F.C. acknowledges the financial support from the National Natural Science Foundation of China (grant no. 51975204) and the Natural Science Foundation of Hunan Province, China (grant no. 2021JJ30103). H.L. is grateful for the funding of China Scholarship Council (CSC)