MicroRNA-197 Promotes Metastasis of Hepatocellular Carcinoma by Activating Wnt/β-Catenin Signaling

Background/Aims: MicroRNA-197 (miR-197) has been shown to play roles in epithelialmesenchymal transition (EMT) and metastasis. The Wnt/β-catenin pathway is associated with EMT, but whether miR-197 regulatesWnt/β-catenin remains unclear. This study was to demonstrate the role of miR-197 on the Wnt/β-catenin pathway in hepatocellular carcinoma (HCC). Methods: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-197 in 105 HCC specimens and 15 HCC cell lines. We tested the predicted target gene of miR-197 using a genetic report system. The role of miR-197 in HCC cell invasion and migration (wound healingand cell invasion and migrationby Transwell assays) and in an HCC xenograft modelwas analyzed. Results: Using a miRNA microarray analysis of HCC specimens and compared with non-metastatic HCC, miR-197 was identified as one of the most upregulated miRNAs in metastatic HCC. miR-197 expression was positively associated with the invasiveness of HCC cell lines. Metastatic HCC cells with high miR-197 expression had Wnt/β-catenin signaling activation. High levels of miR-197 expression also promoted EMT and invasionHCC cells in vitro and in vivo. miR-197 directly targeted Axin-2, Naked cuticle 1 (NKD1), and Dickkopf-related protein 2 (DKK2), leading to inhibition of Wnt/β-catenin signaling. High miR-197 expression was found in HCC specimens from patients with portal vein metastasis;high miR-197 expression correlated to the expression of Axin2, NKD1, and DKK2. Conclusion: miR-197 promotes HCC invasion and metastasis by activating Wnt/β-catenin signaling. miR-197 could possibly be used as a prognostic marker and therapeutic target for HCC

Meroterpenoids from Ganoderma Species: A Review of Last Five Years

Abstract Meroterpenoids are hybrid natural products that partially originate from the terpenoid pathway. Ganoderma meroterpenoids (GMs) are a type of meroterpenoids containing a 1,2,4-trisubstituted phenyl and a polyunsaturated terpenoid part. Over last 5 years, great efforts have been made to conduct phytochemistry research on the genus Ganoderma, which have led to the isolation and identification of a number of GMs. These newly reported GMs showed diverse structures and a wide range of biological activities. This review gives an overview of new GMs from genus Ganoderma and their biological activities and biosynthetic pathway, focusing on the period from 2013 until 2018. Graphical Abstrac

Comparison of in vitro – in vivo extrapolation of biliary clearance using an empirical scaling factor versus transport‐based scaling factors in sandwich‐cultured rat hepatocytes

Biliary clearance (CL b ) is often underestimated by in vitro – in vivo extrapolation from sandwich‐cultured hepatocytes (SCHs). The objective of this study was to compare the performance of a universal correction factor with transporter‐based correction factors in correcting underestimation of CL b . The apparent in vitro CL b of a training set of 21 compounds was determined using the SCH model and extrapolated to apparent in vivo CL b (CL b, app ). A universal correction factor (10.2) was obtained by a linear regression of the predicted CL b, app and observed in vivo CL b of training set compounds and applied to an independent test set ( n = 20); the corrected CL b predictions of 13 compounds were within twofold error of observed values. Furthermore, two transporter‐based correction factors (Organic anion transporting polypeptides/multidrug‐resistance‐related protein 2 and diffusion/P‐glycoprotein) were estimated by linear regression analysis of training set compounds. The applications of the two correction factors to the test set resulted in improved prediction precision. In conclusion, both the universal correction factor and transporter‐based correction factors provided reasonable corrections of CL b values, which are often underestimated by the SCH model. The use of transporter‐based correction factors resulted in an even greater improvement of predictions for compounds with intermediate‐to‐high CL b values. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 102:2837–2850, 2013Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/99050/1/23620_ftp.pd

Macathiohydantoin L, a Novel Thiohydantoin Bearing a Thioxohexahydroimidazo [1,5-a] Pyridine Moiety from Maca (Lepidium meyenii Walp.)

Five new thiohydantoin derivatives (1–5) were isolated from the rhizomes of Lepidium meyenii Walp. NMR (1H and 13C NMR, 1H−1H COSY, HSQC, and HMBC), HRESIMS, and ECD were employed for the structure elucidation of new compounds. Significantly, the structure of compound 1 was the first example of thiohydantoins with thioxohexahydroimidazo [1,5-a] pyridine moiety. Additionally, compounds 2 and 3 possess rare disulfide bonds. Except for compound 4, all isolates were assessed for neuroprotective activities in corticosterone (CORT)-stimulated PC12 cell damage. Among them, compound (−)-3 exhibited moderate neuroprotective activity (cell viability: 68.63%, 20 μM) compared to the positive control desipramine (DIM) (cell viability: 88.49%, 10 μM)

Numerical Investigation of Natural Convection in an Open-Ended Square Channel with Two Suspending Heat Sources

Passive heat dissipation cooling technologies based on natural convection in open channels can effectively control the maximum temperature and improve the temperature homogeneity of 5G base stations, data centers and other equipment. In this paper, the flow and heat transfer of natural convection in an open-ended square channel with two suspending heat sources are studied through numerical simulation. The distributions of the temperature field and flow field in the channel with different horizontal distances and vertical altitude differenced of the heat sources are acquired via the finite element method (FEM)-based COMSOL Multiphysics. The changes in local temperature and the local Nusselt number are obtained. The relationships between the temperature field, flow field, and Nusselt number with respect to the geometric parameters of the heat sources are discussed. With different geometric parameters of the two suspending heat sources, the average surface temperature at the bottom is always lower than the top, while the average Nusselt number reaches maximum and minimum values at the bottom and top surfaces, respectively. As the horizontal distance increases, the maximum vertical airflow velocity decreases. The average surface temperature and local Nusselt number go through a V-shape and reverse V-shape tendency, respectively. The maximum temperature at the surface of the heat source is 397 K at a horizontal distance of 0.36 m. The local Nusselt number on the side of the heat source reaches its maximum at a horizontal distance of 0.28 m with an average value of 33.5. As the vertical altitude difference increases, the temperature difference between the heat sources increases from 0 K to 54 K, and the maximum vertical airflow velocity goes through a reverse V-shape tendency. The Nusselt number of the right heat source decreases to a certain value of about 20, while that of the left heat source goes through a fluctuating tendency. The results show that the best arrangement of the heat sources is a vertical altitude difference of 0 m and a horizontal distance of 0.28 m

New Phenolic Dimers from Plant <i>Paeonia suffruticosa</i> and Their Cytotoxicity and NO Production Inhibition

The Paeonia suffruticosa, known as ‘Feng Dan’, has been used for thousands of years in traditional Chinese medicine. In our chemical investigation on the root bark of the plant, five new phenolic dimers, namely, paeobenzofuranones A–E (1–5), were characterized. Their structures were determined using spectroscopic analysis including 1D and 2D NMR, HRESIMS, UV, and IR, as well as ECD calculations. Compounds 2, 4, and 5 showed cytotoxicity against three human cancer cell lines, with IC50 values ranging from 6.7 to 25.1 μM. Compounds 1 and 2 showed certain inhibitory activity on NO production. To the best of our knowledge, the benzofuranone dimers and their cytotoxicity of P. suffruticosa are reported for the first time in this paper

A Highly Accurate Positioning Solution for C-V2X Systems

Cellular vehicle-to-everything (C-V2X) is essential in enabling safe, reliable, and efficient transportation services. It serves as serve as the foundation for vehicles to communicate with each other and everything around them. One fundamental element in C-V2X is positioning, namely extracting the vehicle’s absolute and relative positions concerning other objects such as buildings, pedestrians, traffic signs, and other vehicles. However, its feasibility in enabling vehicular positioning has not been fully explored yet. In this paper, key performance indicators (KPIs) for C-V2X positioning have been described firstly. Then positioning challenges and conventional positioning methods for C-V2X are reviewed. Afterward, two user equipment (UE)-based and UE-assisted C-V2X positioning architectures are proposed, and key technologies are also described. Lastly, testing and typical application cases are provided

A Highly Accurate Positioning Solution for C-V2X Systems

Cellular vehicle-to-everything (C-V2X) is essential in enabling safe, reliable, and efficient transportation services. It serves as serve as the foundation for vehicles to communicate with each other and everything around them. One fundamental element in C-V2X is positioning, namely extracting the vehicle’s absolute and relative positions concerning other objects such as buildings, pedestrians, traffic signs, and other vehicles. However, its feasibility in enabling vehicular positioning has not been fully explored yet. In this paper, key performance indicators (KPIs) for C-V2X positioning have been described firstly. Then positioning challenges and conventional positioning methods for C-V2X are reviewed. Afterward, two user equipment (UE)-based and UE-assisted C-V2X positioning architectures are proposed, and key technologies are also described. Lastly, testing and typical application cases are provided

Anaerobic germination of green coffee beans: A novel strategy to improve the quality of commercial Arabica coffee

This study aimed to improve the brewing quality of commercial Arabica coffee through anaerobic germination. Changes in important compounds and cupping scores of germination roasting coffee with different germination degrees were investigated by 1H NMR, HS-SPME-GC-MS and sensory analysis. Statistical analysis of multivariate analysis results indicated that 6 water-soluble chemical components and 8 volatile chemical components have the potential to be markers of germinated roasting coffee. In addition, germination significantly reduced caffeine content and acrylamide formation in roasted coffee. Sensory analysis according to the Specialty Coffee Association (SCA) cupping protocol demonstrated that anaerobic germination modified flavor attributes, improved the quality, and increased sensory scores. Furthermore, anaerobic sprouting increased fruity descriptors, but over-sprouting did not improve overall attributes while producing both fermentative and vegetable descriptors. Therefore, suitable anaerobic germination of green coffee beans can be used as a new strategy to improve the flavor of commercial Arabica coffee