Intraspecific variation in shoot flammability in Dracophyllum rosmarinifolium is not predicted by habitat environmental conditions

Background: Flammability is a compound plant trait that can vary significantly across natural populations within species. Investigating intraspecific variation in flammability provides insights into the evolution of plant flammability and inform understanding of wildfire risk and behaviour in different habitats. Methods: We measured four flammability variables, representing ignitibility (time to ignition), sustainability (total burning time), combustibility (maximum temperature during burning) and consumability (percentage of biomass consumed by fire) to assess the shoot-level flammability of Dracophyllum rosmarinifolium (G. Forst.) (Ericaceae), a polymorphic endemic species distributed throughout New Zealand. We examined the relationship between flammability components and a suite of climatic and geographic variables (elevation, latitude, mean annual temperature (MAT), mean annual rainfall (MAR) of the sample locations, etc.). Results: We measured shoot-level flammability components of 62 individuals across eight populations. Burning time, maximum temperature and burnt biomass were positively correlated with each other, while ignition score was independent of other flammability components. All flammability components varied significantly across the eight populations. The habitat conditions we considered were not related to any of the shoot-level flammability components of D. rosmarinifolium. Conclusions: Intraspecific variation in flammability in D. rosmarinifolium may be a byproduct of selection on other functional traits, such as leaf size, shoot lipid content, indicating that plant flammability is an incidental result, rather than selected for, at least in ecosystems without fire as a selective force

miR-183/96/182 Cluster Regulates the Development of Bovine Myoblasts through Targeting <i>FoxO1</i>

Muscle development is an important factor affecting meat yield and quality and is coordinated by a variety of the myogenic genes and signaling pathways. Recent studies reported that miRNA, a class of highly conserved small noncoding RNA, is actively involved in regulating muscle development, but many miRNAs still need to be further explored. Here, we identified that the miR-183/96/182 cluster exhibited higher expression in bovine embryonic muscle; meanwhile, it widely existed in other organizations. Functionally, the results of the RT-qPCR, EdU, CCK8 and immunofluorescence assays demonstrated that the miR-183/96/182 cluster promoted proliferation and differentiation of bovine myoblast. Next, we found that the miR-183/96/182 cluster targeted FoxO1 and restrained its expression. Meanwhile, the expression of FoxO1 had a negative correlation with the expression of the miR-183/96/182 cluster during myoblast differentiation. In a word, our findings indicated that the miR-183/96/182 cluster serves as a positive regulator in the proliferation and differentiation of bovine myoblasts through suppressing the expression of FoxO1

The Response of Mesofauna to Nitrogen Deposition and Reduced Precipitation during Litter Decomposition

Soil mesofauna plays an important role in decomposing organic matter, recycling nutrients, and increasing nutrient availability. The effects of nitrogen (N) deposition and reduced precipitation on the litter-dwelling mesofaunal community and how this process affects litter decomposition remain poorly understood. Herein, a two-year simulated N deposition and throughfall reduction experiment was carried out in a natural evergreen broad-leaved subtropical forest to examine the effects of N deposition and reduced precipitation on soil mesofauna during litter decomposition. Four treatments were established: control (CK), N deposition (N), reduced precipitation (RP), and combined N deposition and reduced precipitation (N + RP). We collected and identified 19,782 individuals of mesofauna in litterbags during the whole experiment. Mites (Prostigmata, Mesostigmata, and Oribatida) and Collembola comprised almost 90% of the total number of individuals collected and dominated the soil mesofauna in our study. Our results revealed the negative effects of N deposition on the density of Oribatida mites and Collembola and the total density of soil mesofauna. Reduced precipitation significantly increased the density of Collembola and Oribatida mites and the total density of mesofauna and marginally significantly increased the density of Mesostigmata mites but decreased the diversity of mesofauna. The interaction effects of N deposition and reduced precipitation significantly affected the density of Prostigmata mites, Oribatida mites, Collembola, and the diversity of mesofauna. N deposition combined with reduced precipitation significantly inhibited litter decomposition, whereas no significant interaction effects were observed. Furthermore, correlation analysis indicated that litter mass loss was significantly positively correlated with the density of Prostigmata, Mesostigmata, and Oribatida, as well as the diversity of mesofauna. Overall, during the two-year decomposition process, our results suggest that N deposition and reduced precipitation interactively affected mesofaunal diversity and that N deposition adversely affected the mesofaunal community, while reduced precipitation increased the density of some groups but decreased mesofaunal diversity, consequently cascading on the decomposition of leaf litter

Downregulation of LncRNA GCLC-1 Promotes Microcystin-LR-Induced Malignant Transformation of Human Liver Cells by Regulating GCLC Expression

Microcystin-LR (MCLR) is an aquatic toxin, which could lead to the development of hepatocellular carcinoma (HCC). Long non-coding RNAs (lncRNAs) are considered important regulatory elements in the occurrence and development of cancer. However, the roles and mechanisms of lncRNAs during the process of HCC, induced by MCLR, remain elusive. Here, we identified a novel lncRNA, namely lnc-GCLC-1 (lncGCLC), which is in close proximity to the chromosome location of glutamate&ndash;cysteine ligase catalytic subunit (GCLC). We then investigated the role of lncGCLC in MCLR-induced malignant transformation of WRL68, a human hepatic cell line. During MCLR-induced cell transformation, the expression of lncGCLC and GCLC decreased continuously, accompanied with a consistently high expression of miR-122-5p. Knockdown of lncGCLC promoted cell proliferation, migration and invasion, but reduced cell apoptosis. A xenograft nude mouse model demonstrated that knockdown of lncGCLC promoted tumor growth. Furthermore, knockdown of lncGCLC significantly upregulated miR-122-5p expression, suppressed GCLC expression and GSH levels, and enhanced oxidative DNA damages. More importantly, the expression of lncGCLC in human HCC tissues was significantly downregulated in the high-microcystin exposure group, and positively associated with GCLC level in HCC tissues. Together, these findings suggest that lncGCLC plays an anti-oncogenic role in MCLR-induced malignant transformation, by regulating GCLC expression

Copy Number Variations and Expression Levels of Guanylate-Binding Protein 6 Gene Associated with Growth Traits of Chinese Cattle

Association studies have indicated profound effects of copy number variations (CNVs) on various phenotypes in different species. In this study, we identified the CNV distributions and expression levels of guanylate-binding protein 6 (GBP6) associated with the growth traits of Chinese cattle. The results showed that the phenotypic values of body size and weight of Xianan (XN) cattle were higher than those of Nanyang (NY) cattle. The medium CNV types were mostly identified in the XN and NY breeds, but their CNV distributions were significantly different (adjusted p &lt; 0.05). The association analysis revealed that the body weight, cannon circumference and chest circumference of XN cattle had significantly different values in different CNV types (p &lt; 0.05), with CNV gain types (Log22&minus;&Delta;&Delta;Ct &gt; 0.5) displaying superior phenotypic values. We also found that transcription levels varied in different tissues (p &lt; 0.001) and the CNV gain types showed the highest relative gene expression levels in the muscle tissue, consistent with the highest phenotypic values of body weight and cannon circumference among the three CNV types. Consequently, our results suggested that CNV gain types of GBP6 could be used as the candidate markers in the cattle-breeding program for growth traits

Ambient catalyst free nitrogen fixation with water dimer radical cation

The growth and sustainable development of humanity is heavily dependent upon the process of fixing nitrogen (N2) to ammonia (NH3). However, the currently adopted methods are associated with severe environmental hazards and tremendous energy costs, which limit their sustainability and profitability. Herein we discovered a catalyst-free disproportionation reaction of N2 by water dimer radical cation, (H2O)2+•, which occurs under mild ambient conditions via distinctive HONH-HNOH+• intermediate to yield economically valuable nitroxyl (HNO) and hydroxylamine (NH2OH) products, in alternative to NH3. Calculations suggest that the reaction is prompted by the coordination of electronically excited N2 with (H2O)2+• in its two-center-three-electron (2c-3e) configuration. Subsequent excited-state double proton transfer leads to one-step water addition to N2. The ambient fixation of N2 into HNO and NH2OH with high selectivity offers great profitability and total avoidance of polluting emissions, such as CO2 or NOy, thus giving an entirely new look and perspectives to the problem of green N2 fixation

Efficient catalyst-free N2 fixation by water radical cations under ambient conditions

Abstract The growth and sustainable development of humanity is heavily dependent upon molecular nitrogen (N2) fixation. Herein we discover ambient catalyst-free disproportionation of N2 by water plasma which occurs via the distinctive HONH-HNOH+• intermediate to yield economically valuable nitroxyl (HNO) and hydroxylamine (NH2OH) products. Calculations suggest that the reaction is prompted by the coordination of electronically excited N2 with water dimer radical cation, (H2O)2 +•, in its two-center-three-electron configuration. The reaction products are collected in a 76-needle array discharge reactor with product yields of 1.14 μg cm–2 h–1 for NH2OH and 0.37 μg cm–2 h–1 for HNO. Potential applications of these compounds are demonstrated to make ammonia (for NH2OH), as well as to chemically react and convert cysteine, and serve as a neuroprotective agent (for HNO). The conversion of N2 into HNO and NH2OH by water plasma could offer great profitability and reduction of polluting emissions, thus giving an entirely look and perspectives to the problem of green N2 fixation

Hsa_circ_0021205 enhances lipolysis via regulating miR-195-5p/HSL axis and drives malignant progression of glioblastoma

Abstract Abnormal lipid metabolism is an essential hallmark of glioblastoma. Hormone sensitive lipase (HSL), an important rate-limiting enzyme contributed to lipolysis, which was involved in aberrant lipolysis of glioblastoma, however, its definite roles and the relevant regulatory pathway have not been fully elucidated. Our investigations disclosed high expression of HSL in glioblastoma. Knock-down of HSL restrained proliferation, migration, and invasion of glioblastoma cells while adding to FAs could significantly rescue the inhibitory effect of si-HSL on tumor cells. Overexpression of HSL further promoted tumor cell proliferation and invasion. Bioinformatics analysis and dual-luciferase reporter assay were performed to predict and verify the regulatory role of ncRNAs on HSL. Mechanistically, hsa_circ_0021205 regulated HSL expression by sponging miR-195-5p, which further promoted lipolysis and drove the malignant progression of glioblastoma. Besides, hsa_circ_0021205/miR-195-5p/HSL axis activated the epithelial-mesenchymal transition (EMT) signaling pathway. These findings suggested that hsa_circ_0021205 promoted tumorigenesis of glioblastoma through regulation of HSL, and targeting hsa_circ_0021205/miR-195-5p/HSL axis can serve as a promising new strategy against glioblastoma