Multiple Results to Some Biharmonic Problems

We study a nonlinear elliptic problem defined in a bounded domain involving biharmonic operator together with an asymptotically linear term. We establish at least three nontrivial solutions using the topological degree theory and the critical groups

PO-257 Application of biochemical index monitoring in Chinese hearing-impaired table tennis team

  Objective Using the theory of sports biochemistry, we can better complete the formulation of the training plan, the evaluations of training effect and the athlete's fatigue status, the real-time monitoring of training intensity and training amount as well as the function level of athletes and the comprehensive evaluation of nutritional status. To provide some theoretical guidance for the daily training of Chinese hearing-impaired table tennis team and to gradually establish the biochemical monitoring model for the Chinese Deaf Table tennis team, this subject has applied biochemical index monitoring to this team. Methods 2.1 Research Object 8 Athletes (male 4 female 4) of the Chinese hearing-impaired table tennis team were studied. The average height, weight, and age were 174.3±5.1cm, 65.4±11.6kg, 23.5±4.9 years respectively.2.2 Research Methods 2.2.1 Literature To find out the energy-supplying and metabolic characteristics of table tennis as well as the modes to accelerate the recovery period of body fatigue, we have searched a variety of literature on this purpose. The investigation of athletes' nutrition regulation, physical training and rehabilitation and other related research results lays a good foundation for the implementation of the project. 2.2.2 Expert Interview This paper discusses the energy metabolism characteristics of the hearing-impaired table tennis players by discussing with the coaches, athletes and experts and scholars of the national Hearing Impairment project group. The evaluation index and standard of athlete's body function, the method of training load monitoring, the way of nutrition regulation, the means of fatigue recovery and the process of physical training were discussed. 3.2.3 Field Investigation From April, 20th, 2017 to July, 10th, 2017, two researchers have long been in the center of national table tennis training located at Zheng Ding, Hebei province, to complete training monitoring and research services. Results 3 Research Results Phlebotomizing 7 of the Chinese hearing-impaired table tennis players in June,2nd,2017 and June 7th, 2017 respectively, The blood samples were analyzed by the team of experts and researchers in the Sports Physiology and Biochemistry Laboratory in Beijing Sport University. Part of the results was shown below in table 1, table 2. (1) Training intensity monitoring Huang Mengping had a slightly higher creatine kinase, a slight decrease in testosterone, a slight rise in cortisol, a lower testosterone/cortisol value, as well as an insignificant reduction in body function than the previous time. She should focus on the recovery of her body condition after training. In general, the testosterone, cortisol and testosterone/cortisol level in this team is slightly higher than the last time, blood urea lower than the previous. Strengthening the monitoring of exercise and nutrition and continuing to maintain a high body synthesis ability are favorable to intense competition. The overall creatinine level decreased, whereas 4 members of the team had insignificantly high creatinine, especially for Huang Mengping who first had appeared high level of it. Although this condition was not too severe to focus on, we should strengthen the regulation of athlete muscle injury and protect their kidney functions. (2) Monitoring of endocrine system indicators Both males and females had higher testosterone synthesis capabilities. They should continue to take the nutritional supplements. The T/C ratio of Tian Jiping has escalated sharply, but it was lower than that of other players. We should pay attention to his training load and promote the recovery training. (3) Monitoring of Iron Metabolic Index Huang Mengping's hemoglobin value and RBC value have been slightly elevated this time. It is suggested that she should keep on to take nutritional supplements and strengthen aerobic endurance training. Hemoglobin for Wang Zhe and Lin Huan (slightly decreased this time) was higher than their counterparts. The ability to transport oxygen was excellent, and it is agreeable to keep on. They need to continue to maintain and promote the body's oxygen reserve capacity. At the same time, they can also upgrade their overall red blood cell levels. Men have seen a high level of hemoglobin and the number of red blood cells as well as robust capabilities of oxymoron reservation and transportation. It is recommended to enhance aerobic endurance training, mainly improving the aerobic capacity. The serum iron of Tian Jiping was promoted quickly. He needs to build up the amount of iron nutrient intake and support the body synthesis. The full iron metabolic indicators were normal, but Zhang Chaoyue and Lin Lin Huan have been seen the lack of the abilities to synthesis iron. So we need to pay attention to iron metabolism-related nutrition intake. In general, iron protein level and the capability to reserve iron have declined. It is recommended to take more iron nutrients, Especially for Shi Ce. (4) Food surveillance After the diet regulation, the indicators for the team's blood fat were within the normal range. Zhang Chaoyue and Wang Zhe should control the high-fat food intake, thus reducing the amount of low-density lipoprotein. (5) Surveillance of immune Indicators The white cell, blood cell classification and immunoglobulin (IgG, IgM, IgA) of the team are all normal, Wang Zhe’s immunoglobulin A and Xu You’s immunoglobulin M are slightly low, we should pay attention to protection. Conclusions  Through the overall monitoring and analysis of the biochemical indexes of the leading athletes of Chinese hearing-impaired table tennis team, The characteristics of biochemical indexes of the high-level hearing-impaired table tennis players are mastered. On the basis of the overall characteristics of the individual, we carried out personalized monitoring and evaluation to give coaches and athletes advice on training, rehabilitation and other aspects related to practice. Biochemical tracking in China's hearing-impaired table tennis team has achieved an excellent application results that have been unanimously recognized by the coaches and athletes. &nbsp

Porous Collagen Scaffold Reinforced with Surfaced Activated PLLA Nanoparticles

Porous collagen scaffold is integrated with surface activated PLLA nanoparticles fabricated by lyophilizing and crosslinking via EDC treatment. In order to prepare surface-modified PLLA nanoparticles, PLLA was firstly grafted with poly (acrylic acid) (PAA) through surface-initiated polymerization of acrylic acid. Nanoparticles of average diameter 316 nm and zeta potential −39.88 mV were obtained from the such-treated PLLA by dialysis method. Porous collagen scaffold were fabricated by mixing PLLA nanoparticles with collagen solution, freeze drying, and crosslinking with EDC. SEM observation revealed that nanoparticles were homogeneously dispersed in collagen matrix, forming interconnected porous structure with pore size ranging from 150 to 200 μm, irrespective of the amount of nanoparticles. The porosity of the scaffolds kept almost unchanged with the increment of the nanoparticles, whereas the mechanical property was obviously improved, and the degradation was effectively retarded. In vitro L929 mouse fibroblast cells seeding and culture studies revealed that cells infiltrated into the scaffolds and were distributed homogeneously. Compared with the pure collagen sponge, the number of cells in hybrid scaffolds greatly increased with the increment of incorporated nanoparticles. These results manifested that the surface-activated PLLA nanoparticles effectively reinforced the porous collagen scaffold and promoted the cells penetrating into the scaffold, and proliferation

Synergistic and complementary safety supervision mode in coal mines: a case of coal mining companies in China

Učinkovit sigurnosni nadzor jamči sigurnu proizvodnju rudnika ugljena. Međutim, tradicionalni način nadzora u kineskim rudnicima ugljena ne može u potpunosti ostvariti sinergijske i dopunske učinke elemenata (tj. sigurnosne inspektore) sustava sigurnosnog nadzora, što uzrokuje informaciju, primjenu i resurs kao izolirani otočić. Kako bi se prevladali ovi nedostaci i poboljšala učinkovitost sigurnosnog nadzora, u ovom je istraživanju istražena sinergijska i dodatna metoda sigurnosnog nadzora (SCSSM - Synergistic and Complementary Safety Supervision Mode). Nakon analize primjenjivosti sinergijske i dopunske ideje upravljanja, ova studija kreativno je predstavila predloženu ideju sigurnosnog nadzora u rudniku ugljena. Na osnovu nedostataka postojećeg načina sigurnosnog nadzora i prednosti sinergijskog i dopunskog upravljanja, uspostavljen je SCSSM i osigurana njegova nedvojbena metoda rada. Konačno, novi način sigurnosnog nadzora primijenjen je u Xinwen Mining Groupu Xin Julong Co., Ltd. s određenom metodom primjene i usporednom analizom. Rezultati pokazuju da novi način sigurnosnog nadzora koristi manje resursa sigurnosnog nadzora nego tradicionalni. U međuvremenu, poboljšani su učinkovitost provjere i ispravljanja skrivenih opasnosti te ograničeni kapacitet projekta. Štoviše, broj nesreća je smanjen, što ima značajnu sigurnosnu, ekonomsku i društvenu korist. Ova studija može ostvariti sinergijske i dopunske učinke elemenata sigurnosnog nadzora radi poboljšanja razine nadzora nad sigurnošću rudnika ugljena te ima veliku vrijednost popularizacije i primjene.Effective safety supervision guarantees coal mine safety production. However, the traditional supervision mode in Chinese coal mines cannot fully realize the synergistic and complementary effects of elements (i.e., safety inspectors) of safety supervision system, which causes information-isolated island, application-isolated island, and resource-isolated island. To overcome these deficiencies and improve the efficiency of safety supervision, a synergistic and complementary safety supervision mode (SCSSM) was explored in this study. After the applicability analysis of the synergistic and complementary management idea, this study creatively introduced the proposed idea to the safety supervision in the coal mine. On the basis of the deficiencies of the existing safety supervision mode and the advantages of synergistic and complementary management, the SCSSM was then established and its definite operation method was also provided. Finally, the new safety supervision mode was applied in Xinwen Mining Group Xin Julong Co., Ltd. with definite implementation method and comparative analysis. Results show that the new safety supervision mode uses lesser safety supervision resources than the traditional one. Meanwhile, the checking and rectification efficiency of hidden dangers and the project qualified rate are improved. Moreover, the number of accidents is reduced, which has significant safety, economic, and social benefits. This study can realize the synergistic and complementary effects of elements of safety supervision to improve the level of coal mine safety supervision and has high value of popularization and application

Metallic nickel nano- and fine particles induce JB6 cell apoptosis through a caspase-8/AIF mediated cytochrome c-independent pathway

<p>Abstract</p> <p>Background</p> <p>Carcinogenicity of nickel compounds has been well documented. However, the carcinogenic effect of metallic nickel is still unclear. The present study investigates metallic nickel nano- and fine particle-induced apoptosis and the signal pathways involved in this process in JB6 cells. The data obtained from this study will be of benefit for elucidating the pathological and carcinogenic potential of metallic nickel particles.</p> <p>Results</p> <p>Using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, we found that metallic nickel nanoparticles exhibited higher cytotoxicity than fine particles. Both metallic nickel nano- and fine particles induced JB6 cell apoptosis. Metallic nickel nanoparticles produced higher apoptotic induction than fine particles. Western-blot analysis showed an activation of proapoptotic factors including Fas (CD95), Fas-associated protein with death domain (FADD), caspase-8, death receptor 3 (DR3) and BID in apoptotic cells induced by metallic nickel particles. Immunoprecipitation (IP) western blot analysis demonstrated the formation of the Fas-related death-inducing signaling complex (DISC) in the apoptotic process. Furthermore, lamin A and beta-actin were cleaved. Moreover, we found that apoptosis-inducing factor (AIF) was up-regulated and released from mitochondria to cytoplasm. Interestingly, although an up-regulation of cytochrome <it>c </it>was detected in the mitochondria of metallic nickel particle-treated cells, no cytochrome <it>c </it>release from mitochondria to cytoplasm was found. In addition, activation of antiapoptotic factors including phospho-Akt (protein kinase B) and Bcl-2 was detected. Further studies demonstrated that metallic nickel particles caused no significant changes in the mitochondrial membrane permeability after 24 h treatment.</p> <p>Conclusion</p> <p>In this study, metallic nickel nanoparticles caused higher cytotoxicity and apoptotic induction than fine particles in JB6 cells. Apoptotic cell death induced by metallic nickel particles in JB6 cells is through a caspase-8/AIF mediated cytochrome <it>c</it>-independent pathway. Lamin A and beta-actin are involved in the process of apoptosis. Activation of Akt and Bcl-2 may play an important role in preventing cytochrome <it>c </it>release from mitochondria to the cytoplasm and may also be important in the carcinogenicity of metallic nickel particles. In addition, the results may be useful as an important reference when comparing the toxicities of different nickel compounds.</p