Polymerase chain reaction and DNA sequence of rainbow trout tumour suppressor gene

A protocol for the extraction of genomic DNA from teleost fish was developed and produced high molecular weight DNA from English sole, white sucker and rainbow trout. A protocol for polymerase chain reaction (PCR) of tumour suppressor gene p53 from rainbow trout genomic DNA was developed. Several primers were chosen inside exon 5 and 9 and exon 6 and 7. A successful PCR method yielded the conserved exon 5 to 9 region of genomic rainbow trout p53 in two fragments: fragment 1 comprising exons 5 and 6 and fragment 2 spanning the region of exons 7 to 9. The apparent molecular weight of fragments 1 and 2 were 510 and 760 base pairs respectively. The sequences of both fragments including the introns were determined and gave 515 and 793 bp for the two PCR fragments. These sequences will facilitate examination of tumour suppressor gene p53 conserved exons from genomic DNA e.g. for study of p53 mutations in fish

Web-cam as an Easy-to-Use Way for Distant Collaborative Activities

Web-cam is a decade-old technology, but it has received major impetus from recent advancements in both software and hardware. Here we report our experiences from using off-the-shelf web-cam tools for various configurations in collaborative ventures. They span from Macintosh to PC in hardware and IchatAV and SquidCam in software. We work from various locations in the U.S. One prominent impediment is the presence of firewalls in networks. We summarize the results from the various combinations of parties involved by a matrix. Web-cams can be utilized today as an economical and viable means of point-to-point communication for the public. Greater bandwidth is sorely needed for multi-party conferencing on the present Internet network. We then propose Web-cam can be utilized as a web service for facilitating collaborative research, using the newly developed middleware coined Narada-Brokering. We expe ct that web-cam will play an important role in geoinformatics. 1 I