Exploring transcriptional signalling mediated by OsWRKY13, a potential regulator of multiple physiological processes in rice

BACKGROUND Rice transcription regulator OsWRKY13 influences the functioning of more than 500 genes in multiple signalling pathways, with roles in disease resistance, redox homeostasis, abiotic stress responses, and development. RESULTS To determine the putative transcriptional regulation mechanism of OsWRKY13, the putative cis-acting elements of OsWRKY13-influenced genes were analyzed using the whole genome expression profiling of OsWRKY13-activated plants generated with the Affymetrix GeneChip Rice Genome Array. At least 39 transcription factor genes were influenced by OsWRKY13, and 30 of them were downregulated. The promoters of OsWRKY13-upregulated genes were overrepresented with W-boxes for WRKY protein binding, whereas the promoters of OsWRKY13-downregulated genes were enriched with cis-elements putatively for binding of MYB and AP2/EREBP types of transcription factors. Consistent with the distinctive distribution of these cis-elements in up- and downregulated genes, nine WRKY genes were influenced by OsWRKY13 and the promoters of five of them were bound by OsWRKY13 in vitro; all seven differentially expressed AP2/EREBP genes and six of the seven differentially expressed MYB genes were suppressed by in OsWRKY13-activated plants. A subset of OsWRKY13-influenced WRKY genes were involved in host-pathogen interactions. CONCLUSION These results suggest that OsWRKY13-mediated signalling pathways are partitioned by different transcription factors. WRKY proteins may play important roles in the monitoring of OsWRKY13-upregulated genes and genes involved in pathogen-induced defence responses, whereas MYB and AP2/EREBP proteins may contribute most to the control of OsWRKY13-downregulated genes.This work was supported by grants from the National Program of High Technology Development of China, the National Program on the Development of Basic Research in China, and the National Natural Science Foundation of China

Exploring transcriptional signalling mediated by OsWRKY13, a potential regulator of multiple physiological processes in rice

<p>Abstract</p> <p>Background</p> <p>Rice transcription regulator OsWRKY13 influences the functioning of more than 500 genes in multiple signalling pathways, with roles in disease resistance, redox homeostasis, abiotic stress responses, and development.</p> <p>Results</p> <p>To determine the putative transcriptional regulation mechanism of OsWRKY13, the putative <it>cis</it>-acting elements of OsWRKY13-influenced genes were analyzed using the whole genome expression profiling of <it>OsWRKY13</it>-activated plants generated with the Affymetrix GeneChip Rice Genome Array. At least 39 transcription factor genes were influenced by OsWRKY13, and 30 of them were downregulated. The promoters of OsWRKY13-upregulated genes were overrepresented with W-boxes for WRKY protein binding, whereas the promoters of OsWRKY13-downregulated genes were enriched with <it>cis</it>-elements putatively for binding of MYB and AP2/EREBP types of transcription factors. Consistent with the distinctive distribution of these <it>cis</it>-elements in up- and downregulated genes, nine WRKY genes were influenced by OsWRKY13 and the promoters of five of them were bound by OsWRKY13 <it>in vitro</it>; all seven differentially expressed AP2/EREBP genes and six of the seven differentially expressed MYB genes were suppressed by in <it>OsWRKY13</it>-activated plants. A subset of OsWRKY13-influenced WRKY genes were involved in host-pathogen interactions.</p> <p>Conclusion</p> <p>These results suggest that OsWRKY13-mediated signalling pathways are partitioned by different transcription factors. WRKY proteins may play important roles in the monitoring of OsWRKY13-upregulated genes and genes involved in pathogen-induced defence responses, whereas MYB and AP2/EREBP proteins may contribute most to the control of OsWRKY13-downregulated genes.</p

Wind Power, Hydropower and Thermal Power Combined Low-Carbon Maintenance Optimization Based on Continuous Hidden Markov Model

[Introduction] In the context of the new power system, low-carbon maintenance of wind turbines and coordinated maintenance with conventional wind turbine generator systems (WTGS) need to be solved urgently. In this paper, taking into account the impact of multi-attribute meteorological factors and low carbon and economic needs, an optimization model for wind power, hydropower and thermal power combined low-carbon maintenance based on continuous hidden Markov model is established. [Method] Firstly, dynamic tracking of wind farm maintenance capacity was realized by taking rainfall, wind speed and lightning hazard degree as the observation sequence, taking maintenance capacity as hidden state sequence, and using continuous hidden Markov model (CHMM) process. Then, an optimization model for wind power, hydropower and thermal power combined low-carbon maintenance was constructed by taking the optimal maintenance capacity as the decision-making basis, taking the minimum total cost as the optimization objective, and taking the maintenance constraints and system control constraints into consideration. Finally, took the IEEE30-node system as an example. [Result] The results show that the proposed model has more significant economic benefits and low carbon characteristics. [Conclusion] The research in this paper has high theoretical value for the operation and maintenance of WTGS, and has strong engineering applicability

Predictors of permanent pacemaker implantation in aortic valve diseases after TAVI with vitaFlow liberty system

IntroductionPermanent pacemaker implantation (PPI) is a known complication in patients with aortic stenosis following transcatheter aortic valve implantation (TAVI). However, there is limited research on TAVI for pure aortic regurgitation (PAR), and more investigation is needed to determine the occurrence of postoperative cardiac conduction block and the need for PPI in this population. Therefore, this retrospective analysis aimed to evaluate the incidence of cardiac conduction block and the necessity of PPI after TAVI in patients with different types of aortic valve disease, including pure aortic stenosis (PAS), aortic stenosis with regurgitation (ASR), and PAR.MethodsClinical data of 100 patients who TAVI were analyzed retrospectively. The incidence of conduction block was assessed, and clinical factors were examined to predict the necessity of PPI.ResultsCardiac conduction block was found to be a common complication following TAVI, particularly in patients with PAR. PAR was identified as an independent risk factor for requiring PPI. Additionally, first-degree atrioventricular block emerged as a sensitive predictor for PPI in patients with PAR.DiscussionThese findings provide valuable insights into the safety and effectiveness of TAVI, which can help enhance patient management and reduce complications

Communication security of autonomous ground vehicles based on networked control systems: The optimized LMI approach

The paper presents a study of networked control systems (NCSs) that are subjected to periodic denial-of-service (DoS) attacks of varying intensity. The use of appropriate Lyapunov–Krasovskii functionals (LKFs) help to reduce the constraints of the basic conditions and lower the conservatism of the criteria. An optimization problem with constraints is formulated to select the trigger threshold, which is solved using the gradient descent algorithm (GDA) to improve resource utilization. An intelligent secure event-triggered controller (ISETC) is designed to ensure the safe operation of the system under DoS attacks. The approach is validated through experiments with an autonomous ground vehicle (AGV) system based on the Simulink platform. The proposed method offers the potential for developing effective defense mechanisms against DoS attacks in NCSs

Double-blind comparison of ziprasidone and risperidone in the treatment of Chinese patients with acute exacerbation of schizophrenia

Background: The aim of the study was to evaluate the efficacy and safety of ziprasidone versus risperidone in Chinese subjects with acute exacerbation of schizophrenia. Methods: In patients meeting the Chinese Classification of Mental Disorders criteria for schizophrenia and with a Positive and Negative Syndrome Scale (PANSS) total score &gt;= 60 were randomly assigned to six weeks of double-blind treatment with ziprasidone 40-80 mg twice daily or risperidone 1-3 mg bid, flexibly dosed. Noninferiority was demonstrated if the upper limit of the two-sided 95% confidence interval (CI) for the difference in PANSS total score improvement from baseline in the evaluable population was smaller than the prespecified noninferiority margin of 10 units. Results: The intent-to-treat population comprised 118 ziprasidone-treated and 121 risperidone-treated subjects. Improvement (reduction) from baseline to week 6 in PANSS total score was (-35.6 [95% CI: -38.6, -32.6]) for ziprasidone and (-37.1 [95% CI: -39.9, -34.4]) for risperidone. Noninferiority was demonstrated in the evaluable population with a difference score of 1.5 [95% CI: -2.5, 5.5]. Mean prolactin levels decreased at week 6 compared with baseline for ziprasidone (-3.5 ng/mL), but significantly increased for risperidone (61.1 ng/mL; P &lt; 0.001). More risperidone-treated subjects (14.9%) than ziprasidone-treated subjects (4.2%) reported weight gain &gt;= 7%. Akathisia and somnolence in the ziprasidone group and akathisia and insomnia in the risperidone group were the most common side effects. Treatment-related/treatment-emergent adverse events were reported by 79.7% and 71.1% of ziprasidone-treated and risperidone-treated subjects, respectively. Conclusion: In Chinese subjects, ziprasidone was as effective as risperidone, with less weight gain and less prolactin elevation.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000294955100009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Clinical NeurologyPsychiatrySCI(E)PubMed3ARTICLE77-85

Global Infectious Diseases in July 2023: Monthly Analysis

Many infectious diseases are ubiquitous and pose persistent adverse effects on public health. Infectious diseases have also been leading causes of high mortality in different periods of history. Real-time monitoring and analysis of global infectious disease transmission can provide a comprehensive understanding of critical information regarding the transmission routes, scope, velocity, and effects of viruses or bacteria. Here, using Shusi Tech’s Global Epidemic Information Monitoring System, we analyzed the prevalence of infectious diseases worldwide. We describe types of infectious diseases with relatively low incidence from 24 June 2023 to 23 July 2023 as comprehensibly as possible

Comparative analysis of sequencing technologies for single-cell transcriptomics.

Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq platform for scRNA-seq. We generate a resource of 468 single cells and 1297 matched single cDNA samples, performing SMARTer and Smart-seq2 protocols on two cell lines with RNA spike-ins. We sequence these libraries on both platforms using single- and paired-end reads. The platforms have comparable sensitivity and accuracy in terms of quantification of gene expression, and low technical variability. Our study provides a standardized scRNA-seq resource to benchmark new scRNA-seq library preparation protocols and sequencing platforms

Jugoslavija u međunarodnoj trgovini ribom, ribljim proizvodima i prerađevinama

Sulfonamides are profoundly important in pharmaceutical design. C–N cross-coupling of sulfonamides is an effective method for fragment coupling and structure–activity relationship (SAR) mining. However, cross-coupling of the important <i>N</i>-arylsulfonamide pharmacophore has been notably unsuccessful. Here, we present a solution to this problem via oxidative Cu-catalysis (Chan–Lam cross-coupling). Mechanistic insight has allowed the discovery and refinement of an effective cationic Cu catalyst to facilitate the practical and scalable Chan–Lam <i>N</i>-arylation of primary and secondary <i>N</i>-arylsulfonamides at room temperature. We also demonstrate utility in the large scale synthesis of a key intermediate to a clinical hepatitis C virus treatment