Primary adenomyoepithelioma of tonsil

We present a case of adenomyoepithlioma (AME) arising from the tonsil. AME is an uncommon tumor that typically arises in breast, but rarely found in salivary glands, lung, and skin. Its biological features have not been thoroughly characterized. Here we describe a primary AME originating from the tonsil. The pathologic changes were characterized by hypercellularity, the dominance of both epithelial and myoepithelial cells. Malignancy was evidenced by the presence of a high mitotic rate and invasive growth. The epithelial cells express high levels of cytokeratin and epithelial membrane antigen (EMA). The myoepithelial cells show positive staining for calponin, p63, vimentin, and S-100. A thorough review of the literature indicates that this is likely the first reported case of AME from the tonsil. Following descriptions of the diagnosis, treatment, and prognosis of this specific case, pathologic and clinical characteristics of AME from other tissues are also compiled and discussed

The complete chloroplast genome of Tulipa gesneriana (Liliaceae) and its phylogenetic analysis

The complete chloroplast genome sequence of Tulipa gesneriana L. was determined to investigate its phylogenetic position. This plastome is 151,958 base pairs (bp) in length, and comprises two inverted repeat (IRa and IRb) regions of 26,352 bp, a small single-copy region of 17,123 bp and a large single-copy region of 82,131 bp. The GC contents of the cp genome were 36.6%. In total, we annotated 126 genes including 81 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis based on nine chloroplast genomes indicates that T. gesneriana is closely related to T. iliensis and T. thianschanica

Distribution of Carboniferous source rocks and petroleum systems in the Junggar Basin

With pre-Cambrian continental nuclei and Early Paleozoic accretionary folded belts as the collaged basement, the Junggar Basin underwent an evolution of intra-cratonic basin after the transitional period of Carboniferous to Permian. The Carboniferous Period was a critical phase for the cratonization of Junggar Terrane, with two continental passive margins developed on both the southern and northern margins, and with rifts and upswells in the basin interior. The rifts were full-filled with volcanic clastic rocks up to 2 000 to 5 000 m in thickness. Influenced by the marine transgression, the regional extended source-rocks of the Lower Carboniferous Dishuiquan Formation(C1d) and the Upper Carboniferous Batamayneishan Formation(C2b) occurred within or around the basin, with the former one as oil and gas source rocks, while the latter one as effective gas source rocks. Volcanic rocks in the Batamayneishan Formation are the main reservoirs. Two petroleum systems were formed: C1d-C (.) and C2b-C2b (!). The tectonic events in the Late Jurassic and Neogene gave rise to the formation, modification, and finalizing of the Carboniferous prospects. The difference between the evolutionary process of each structural unit resulted in the differentiation of the petroleum systems horizontally. A series of oil and gas fields have been discovered in Carboniferous, such as the Wucaiwan, Shixi, Kelameili, and Chepaizi oil and gas fields as well as the oil-bearing Block-2, 4, and 6 in the Karamay Oilfield. The exploration has proven that the Carboniferous has great potential for oil and gas discovery. 摘 要: 准噶尔盆地是在前寒武纪陆核和早古生代增生褶皱带的拼合基底之上经历石炭纪—二叠纪过渡阶段后形成的克拉通内坳陷盆地，石炭纪是准噶尔及邻区克拉通化过渡发展的关键时期，在准噶尔地块南、北缘发育了被动大陆边缘，地块内部断陷与凸起相间，断陷内沉积了厚度达2 000～5 000 m的火山碎屑岩系。受海侵范围控制，盆地内部及周缘发育区域展布的石炭系下统滴水泉组和上统巴塔玛依内山组两套有效烃源岩，前者可能是油、气烃源岩，后者是有效的气源岩，上石炭统巴塔玛依内山组火山岩是主要的储集体，由此形成了C1d—C(.)和C2b—C2b(!)两个含油气系统。中侏罗世晚期—晚侏罗世和新近纪以来的构造活动导致了石炭系圈闭的形成、改造与最终定型。不同构造单元后期演化历史的差异导致石炭系含油气系统在平面上产生分异。迄今在石炭系已发现了五彩湾、石西、克拉美丽、克拉玛依二/四/六井区、车排子等多个油气田，油气勘探实践表明准噶尔盆地石炭系具有较好的勘探前景。图8表3参41 Key words: Carboniferous, hydrocarbon source rock, volcanic rocks, petroleum system, Junggar Basi

Identification of m5C-related lncRNAs signature to predict prognosis and therapeutic responses in esophageal squamous cell carcinoma patients

Abstract Esophageal squamous cell carcinoma (ESCC) has a dismal prognosis because of atypical early symptoms and heterogeneous therapeutic responses. 5-methylcytosine (m5C) modification plays an important role in the onset and development of many tumors and is widespread in long non-coding RNA (lncRNA) transcripts. However, the functions of m5C and lncRNAs in ESCC have not been completely elucidated. Herein, this study aimed to explore the role of m5C-related lncRNAs in ESCC. The RNA-seq transcriptome profiles and clinical information were downloaded from the TCGA-ESCC database. Pearson analysis was used to identify m5C-related lncRNAs. Then we established the m5C-related lncRNAs prognostic signature (m5C-LPS) using univariate Cox and least absolute shrinkage and selection operator (LASSO) regression analysis. Then, the prognostic value of m5C-LPS was evaluated internally and externally using the TCGA-ESCC and GSE53622 databases through multiple methods. We also detected the expression of these lncRNAs in ESCC cell lines and patient tissues. Fluorescence in situ hybridization (FISH) was used to detect the prognostic value of specific lncRNA. In addition, clinical parameters, immune status, genomic variants, oncogenic pathways, enrichment pathways, and therapeutic response features associated with m5C-LPS were explored using bioinformatics methods. We constructed and validated a prognostic signature based on 9 m5C-related lncRNAs (AC002091.2, AC009275.1, CAHM, LINC02057.1, AC0006329.1, AC037459.3, AC064807.1, ATP2B1-AS1, and UBAC2-AS1). The quantitative real-time polymerase chain reaction (qRT-PCR) revealed that most lncRNAs were upregulated in ESCC cell lines and patient tissues. And AC002091.2 was validated to have significant prognostic value in ESCC patients. A composite nomogram was generated to facilitate clinical practice by integrating this signature with the N stage. Besides, patients in the low-risk group were characterized by good clinical outcomes, favorable immune status, and low oncogenic alteration. Function enrichment analysis indicated that the risk score was associated with mRNA splicing, ncRNA processing, and DNA damage repair response. At the same time, we found significant differences in the responses to chemoradiotherapy between the two groups, proving the value of m5C-LPS in treatment decision-making in ESCC. This study established a novel prognostic signature based on 9 m5C-related lncRNAs, which is a promising biomarker for predicting clinical outcomes and therapeutic response in ESCC

Mesh model building and migration and accumulation simulation of 3D hydrocarbon carrier system

Migration and accumulation simulation of oil and gas in carrier systems has always been a difficult subject in the quantitative study of petroleum geology. In view of the fact that the traditional geological modeling technology can not establish the interrelation of carriers in three dimensional space, we have proposed a hybrid-dimensional mesh modeling technology consisting of body (stratum), surfaces (faults and unconformities), lines and points, which provides an important research method for the description of geometry of sand bodies, faults and unconformities, the 3D geological modeling of complex tectonic areas, and the simulation of hydrocarbon migration and accumulation. Furthermore, we have advanced a 3D hydrocarbon migration pathway tracking method based on the hybrid-dimensional mesh of the carrier system. The application of this technology in western Luliang Uplift of Junggar Basin shows that the technology can effectively characterize the transport effect of fault planes, unconformities and sand bodies, indicate the hydrocarbon migration pathways, simulate the process of oil accumulation, reservoir adjustment and secondary reservoir formation, predict the hydrocarbon distribution. It is found through the simulation that the areas around the paleo-oil reservoir and covered by migration pathways are favorable sites for oil and gas distribution. Key words: oil and gas migration and accumulation, carrier system, hybrid-dimensional mesh, migration pathway, geological modeling, mesh generation, Junggar Basin, western Luliang Uplif

A method for selecting reference beam model of VMAT plans with three 6MV beam-matched linear accelerators during radiation oncology

Abstract Our objective was to provide a method for selecting reference beam model and evaluating the dosimetric accuracy of volumetric modulated arc therapy (VMAT) plans delivered on three Elekta beam-matched linacs during radiation oncology. Beam data was measured on three beam-matched linacs including Synergy1, Synergy2 and VersaHD. For eighteen lung and esophagus cases, fifty-four plans were generated using VMAT technique with three linac beam models respectively for point dose measurement and three-dimensional dose measurement. Each VMAT plan was executed sequentially on three linacs respectively. Measurement results were compared with treatment planning system (TPS) calculation results for all VMAT plans. Among three beam-matched linacs, discrepancy in beam output factor, percentage depth dose at 5 cm, 10 cm, 20 cm depth and MLC leaf offset are all within 1% except 20 × 20 cm2 and 30 × 30 cm2 field sizes, and discrepancy in beam profile is all within 2%. With comparison between measurement result and TPS calculation result, the absolute dose deviations are within the range of ± 3%, and the gamma passing rates are all over 95% for all VMAT plans, which are within the tolerance of clinical acceptability. Compared with all plans delivered on Synegy1 and VersaHD, the point dose discrepancy between measured results and TPS calculated results for plans delivered on Synergy2 is smallest, and the gamma passing rate between measured results and TPS calculated results for plans delivered on Synergy2 is highest. The beam-matched linacs demonstrate good agreement between measurement result and TPS calculation result for VMAT plans. The method can be used for selecting reference beam model for VMAT plans