Aedes albopictus salivary proteins adenosine deaminase and 34k2 interact with human mast cell specific proteases tryptase and chymase

When mosquitoes probe to feed blood, they inoculate a mixture of salivary molecules into vertebrate hosts' skin causing acute inflammatory reactions where mast cell-derived mediators are involved. Mosquito saliva contains many proteins with largely unknown biological functions. Here, two Aedes albopictus salivary proteins - adenosine deaminase (alADA) and al34k2 - were investigated for their immunological impact on mast cells and two mast cell-specific proteases, the tryptase and the chymase. Mouse bone marrow-derived mast cells were challenged with increased concentrations of recombinant alADA or al34k2 for 1, 3, and 6 h, and to measure mast cell activation, the activity levels of beta-hexosaminidase and tryptase and secretion of IL-6 were evaluated. In addition, a direct interaction between alADA or al34k2 with tryptase or chymase was investigated. Results show that bone marrow-derived mast cells challenged with 10 mu g/ml of alADA secreted significant levels of beta-hexosaminidase, tryptase, and IL-6. Furthermore, both al34k2 and alADA are cut by human tryptase and chymase. Interestingly, al34k2 dose-dependently enhance enzymatic activity of both tryptase and chymase. In contrast, while alADA enhances the enzymatic activity of tryptase, chymase activity was inhibited. Our finding suggests that alADA and al34k2 via interaction with mast cell-specific proteases tryptase and chymase modulate mast cell-driven immune response in the local skin microenvironment. alADA- and al34k2-mediated modulation of tryptase and chymase may also recruit more inflammatory cells and induce vascular leakage, which may contribute to the inflammatory responses at the mosquito bite site

Caring for orphaned children in China /

Includes bibliographical references (pages 245-258) and index

Understanding China’s social policies and practices

AnnaHigh’sprize-winning1bookonChinesenon-governmentalorphanreliefisa valuablecontributiontounderstandingthepracticalimplementationofsocialpolicy andpracticesinChina,bothfromtheperspectiveofthecomplexitiesofthestate andthoseintendedtobenefitfromstatepolicies.AlthoughOrphanRelieffocusesprimarilyonpolicyimplementationandpracticesincaringfororphanedchildren,it reachesmorewidelytochangingstatepoliciesandlawsonnon-governmentalorganizations(NGOs)andchildwelfare,andhowtheselawsandpolicieshavebeenimplementedinpractice,indifferentregions,andatdifferenttimes

In search of civil society: market reform and social change in contemporary China

Since 1978, China has pursued sweeping economic changes in an officially sponsored transition from a Stalinist centrally planned economy to a socialist market economy. China's reformers have highlighted the need to curb the awesome power of the Leninist state and change the balance of power between state and economy, state and society. In practice, the economic reforms have set in train a process of potentially fundamental social and institutional change in China which is creating new socio-economic forces, shifting power in their direction, and raising the possibility of political transformation. This book explores the extent to which this experience can be described and understood in terms of the idea of `civil society', defined in sociological terms as the emergence of an autonomous sphere of voluntary associations capable of organizing the interests of emergent socio-economic groups and counterbalancing the hitherto unchallenged dominance of the Marxist-Leninist state. the authors lay out a clear operational definition of the concept of civil society to make it useful as a tool for empirical inquiry and avoid the cultural relativism of its origins in Western historical experience. Guided by this theoretical framework, the book brings together a vast amount of empirical data on emergent social organization and institutions in contemporary China, drawing on the authors' extensive fieldwork experience in East Asia. It is based on interviews, survey questionnaires, and copious documentary sources, buttressed by in-depth case studies of specific localities over a two-year period from 1991 to 1993. the research focused on the changes in the socio-economic realities of three major social groups - urban manual workers, women, and managers/entrepreneurs. The primary emphasis is on transformations in urban China, though detailed rural case studies of Xiaoshan and Nanhai are included to provide comparative context. The authors describe the new forms of state-society relations, as reflected in the complex links between the state and new associations. They show how the expansion of these associations is jeopardized by the lack of general democratization of China's political institutions

NGOs and accountability in China: child welfare organisations

This book investigates how NGOs in authoritarian states, such as China, craft accountability and legitimacy to ensure their survival. It explores this through the lens of child welfare organisations from 2007 to 2017. The authors provide a fresh approach to accountability that is more attuned to the particular conditions of authoritarianism. The project explores the effects of power relations in shaping the hierarchies of accountability and participation that emerge and the attention given to different voices such as those of donor, government, and users. Essential reading for researchers and policy makers interested in development, NGO, social policy, political science, and child welfare studies