Decreased Filamin b expression regulates trophoblastic cells invasion through ERK/MMP-9 pathway in pre-eclampsia

Objectives: The purpose of this study was to investigate the expression of Filamin b in the placental placenta of patients with early or late onset pre-eclampsia (PE) and its potential effects on the pathophysiology of the disease. Methods and methods: Immunohistochemistry staining, western blot assays and real time PCR were used to detect the expression level of FLN-b. The expression levels of MMP-2, MMP-9 and ERK1/2 proteins from control and FLN-b-silenced JEG-3 cells were also detected by western blot and JEG-3 cell invasion. Results: Compared with normal term pregnancies placentas, the FLN-b expression was significantly lower than that of women with PE, its level in late-onset PE is lower than in early-onset PE. In FLN-b-silenced JEG-3 cells, the protein levels of MMP-2, MMP-9 and phosphorylated ERK1/2 decreased markedly and the number of cells penetrating through the transwell chamber membrane is also greatly reduced. Conclusions: Down-regulation of FLN-b inhibits the ERK/MMP-2 and MMP-9 pathways, leading to trophoblastic invasion disorders in the PE placenta.

Hepatocyte-Specific Deletion of SIRT1 Alters Fatty Acid Metabolism and Results in Hepatic Steatosis and Inflammation

SummaryHepatic metabolic derangements are key components in the development of fatty liver, insulin resistance, and atherosclerosis. SIRT1, a NAD+-dependent protein deacetylase, is an important regulator of energy homeostasis in response to nutrient availability. Here we demonstrate that hepatic SIRT1 regulates lipid homeostasis by positively regulating peroxisome proliferators-activated receptor α (PPARα), a nuclear receptor that mediates the adaptive response to fasting and starvation. Hepatocyte-specific deletion of SIRT1 impairs PPARα signaling and decreases fatty acid β-oxidation, whereas overexpression of SIRT1 induces the expression of PPARα targets. SIRT1 interacts with PPARα and is required to activate PPARα coactivator PGC-1α. When challenged with a high-fat diet, liver-specific SIRT1 knockout mice develop hepatic steatosis, hepatic inflammation, and endoplasmic reticulum stress. Taken together, our data indicate that SIRT1 plays a vital role in the regulation of hepatic lipid homeostasis and that pharmacological activation of SIRT1 may be important for the prevention of obesity-associated metabolic diseases

Effects of OMMT on the aging behaviors of halogen-antimony flame-retarded LGFPA6 composites: Flammability and thermal degradation kinetics

After a long-term thermo-oxidative exposure, compared to the long-glass-fiber reinforced polyamide 6 composite containing halogen-antimony flame retardants (FR/LGFPA6), substitution of a certain content of flame retardants with organo-modified montmorillonite (OMMT) was found to positively affect the FR/LGFPA6 composite in some cases (i.e. flammability and thermal degradation kinetics). The aged OMMT/FR/LGFPA6 composite not only was less flammable but also behaved better in the formation of char residues. All the calculated apparent activation energy obtained by the methods of Friedman, Flynn-Wall-Ozawa and Coats- Redfern in a dynamic measurement showed an obvious increase for the OMMT/FR/LGFPA6 composite after aging, a theoretical evidence for its improved flame retardancy

The jet formation mechanism of Gamma-ray Narrow-line Seyfert 1 Galaxies

Under the coronal magnetic field, we estimate the maximal jet power of the Blandford-\Znajek (BZ) mechanism, Blandford-\Payne (BP) mechanism, and hybrid model. The jet power of the BZ and Hybrid model mechanisms depends on the spin of a black hole, while the jet power of the BP mechanism does not depend on the spin of a black hole. At high black hole spin, the jet power of the hybrid model is greater than that of the BZ and BP mechanisms. We find that the jet power of almost all gamma-\ray narrow line Seyfert 1 galaxies (gamma-\NLS1s) can be explained by the hybrid model. However, one source with jet power 0.1~\1 Eddington luminosity can not be explained by the hybrid model. We suggest that the magnetic field dragged inward by the accretion disk with magnetization-\driven outflows may accelerate the jets in this gamma-\NLS1.Comment: 9 pages,6 figures,accepted for publication in MNRA

General Physical Properties of Gamma-Ray-emitting Radio Galaxies

We study the radio galaxies with known redshift detected by the Fermi satellite after 10 years of data (4FGL-DR2). We use a one-zone leptonic model to fit the quasi-simultaneous multiwavelength data of these radio galaxies and study the distributions of the derived physical parameter as a function of black hole mass and accretion disk luminosity. The main results are as follows. (1) We find that the jet kinetic power of most radio galaxies can be explained by the hybrid jet model based on ADAFs surrounding Kerr black holes. (2) After excluding the redshift, there is a significant correlation between the radiation jet power and the accretion disk luminosity, while the jet kinetic power is weakly correlated with the accretion disk luminosity. (3) We also find a significant correlation between inverse Compton luminosity and synchrotron luminosity. The slope of the correlation for radio galaxies is consistent with the synchrotron self-Compton (SSC) process. The result may suggest that the high-energy component of radio galaxies is dominated by the SSC process.Comment: 9 pages,7 figures, accept for publication in ApJ