Presentation_3_Detection of African swine fever virus antibodies in serum using a pB602L protein-based indirect ELISA.pptx

African Swine Fever (ASF) is an acute, highly contagious and deadly infectious disease that has a huge impact on the swine industry. It is caused by the African swine fever virus (ASFV). The most acute forms of ASF in domestic pigs have mortality rates of up to 100%. The lack of a commercial vaccine and effective therapeutic drugs has brought great challenges to the prevention and control of ASF. Current, the African swine fever virus requires a huge amount of detection, so there is a need for more sensitive and accurate detection technology. The protein pB602L, as a late non-structural protein, has a high corresponding antibody titer and strong antigenicity in infected swine. In this research, the B602L gene was constructed into the pColdI prokaryotic expression vector, and prokaryotic expression of the soluble pB602L protein was induced by IPTG. Western blot analysis demonstrated that the protein had strong immunogenicity. We established an indirect ELISA method for the detection of anti-ASFV using purified recombinant pB602L protein as antigen. The detection method showed excellent specificity without cross-reactions with antibodies against PRRSV, CSFV, JEV, and GETV. The method could detect anti-ASFV in serum samples that were diluted up to 6,400 times, showing high sensitivity. The coefficients of variation of the intra-assay and inter-assay were both <10%. The assays had excellent specificity, sensitivity, and repeatability. In summary, we developed an accurate, rapid, and economical method for the detection of anti-ASFV in pig serum samples with great potential for ASF monitoring and epidemic control.</p

Presentation_2_Detection of African swine fever virus antibodies in serum using a pB602L protein-based indirect ELISA.pptx

African Swine Fever (ASF) is an acute, highly contagious and deadly infectious disease that has a huge impact on the swine industry. It is caused by the African swine fever virus (ASFV). The most acute forms of ASF in domestic pigs have mortality rates of up to 100%. The lack of a commercial vaccine and effective therapeutic drugs has brought great challenges to the prevention and control of ASF. Current, the African swine fever virus requires a huge amount of detection, so there is a need for more sensitive and accurate detection technology. The protein pB602L, as a late non-structural protein, has a high corresponding antibody titer and strong antigenicity in infected swine. In this research, the B602L gene was constructed into the pColdI prokaryotic expression vector, and prokaryotic expression of the soluble pB602L protein was induced by IPTG. Western blot analysis demonstrated that the protein had strong immunogenicity. We established an indirect ELISA method for the detection of anti-ASFV using purified recombinant pB602L protein as antigen. The detection method showed excellent specificity without cross-reactions with antibodies against PRRSV, CSFV, JEV, and GETV. The method could detect anti-ASFV in serum samples that were diluted up to 6,400 times, showing high sensitivity. The coefficients of variation of the intra-assay and inter-assay were both <10%. The assays had excellent specificity, sensitivity, and repeatability. In summary, we developed an accurate, rapid, and economical method for the detection of anti-ASFV in pig serum samples with great potential for ASF monitoring and epidemic control.</p

Presentation_1_Detection of African swine fever virus antibodies in serum using a pB602L protein-based indirect ELISA.pptx

African Swine Fever (ASF) is an acute, highly contagious and deadly infectious disease that has a huge impact on the swine industry. It is caused by the African swine fever virus (ASFV). The most acute forms of ASF in domestic pigs have mortality rates of up to 100%. The lack of a commercial vaccine and effective therapeutic drugs has brought great challenges to the prevention and control of ASF. Current, the African swine fever virus requires a huge amount of detection, so there is a need for more sensitive and accurate detection technology. The protein pB602L, as a late non-structural protein, has a high corresponding antibody titer and strong antigenicity in infected swine. In this research, the B602L gene was constructed into the pColdI prokaryotic expression vector, and prokaryotic expression of the soluble pB602L protein was induced by IPTG. Western blot analysis demonstrated that the protein had strong immunogenicity. We established an indirect ELISA method for the detection of anti-ASFV using purified recombinant pB602L protein as antigen. The detection method showed excellent specificity without cross-reactions with antibodies against PRRSV, CSFV, JEV, and GETV. The method could detect anti-ASFV in serum samples that were diluted up to 6,400 times, showing high sensitivity. The coefficients of variation of the intra-assay and inter-assay were both <10%. The assays had excellent specificity, sensitivity, and repeatability. In summary, we developed an accurate, rapid, and economical method for the detection of anti-ASFV in pig serum samples with great potential for ASF monitoring and epidemic control.</p

Additional file 1: Table S1. of Characteristics and risk factors for 28-day mortality of hospital acquired fungemias in ICUs: data from the EUROBACT study

Baseline characteristics of patients with fungemia. Table S2 Characteristics of fungemia episodes at diagnosis. (DOCX 19 kb

Epidemiology and Outcome of Severe Sepsis and Septic Shock in Intensive Care Units in Mainland China

<div><p>Introduction</p><p>Information about sepsis in mainland China remains scarce and incomplete. The purpose of this study was to describe the epidemiology and outcome of severe sepsis and septic shock in mixed ICU in mainland China, as well as the independent predictors of mortality.</p><p>Methods</p><p>We performed a 2-month prospective, observational cohort study in 22 closed multi-disciplinary intensive care units (ICUs). All admissions into those ICUs during the study period were screened and patients with severe sepsis or septic shock were included.</p><p>Results</p><p>A total of 484 patients, 37.3 per 100 ICU admissions were diagnosed with severe sepsis (n = 365) or septic shock (n = 119) according to clinical criteria and included into this study. The most frequent sites of infection were the lung and abdomen. The overall ICU and hospital mortality rates were 28.7% (n = 139) and 33.5% (n = 162), respectively. In multivariate analyses, APACHE II score (odds ratio[OR], 1.068; 95% confidential interval[CI], 1.027–1.109), presence of ARDS (OR, 2.676; 95%CI, 1.691–4.235), bloodstream infection (OR, 2.520; 95%CI, 1.142–5.564) and comorbidity of cancer (OR, 2.246; 95%CI, 1.141–4.420) were significantly associated with mortality.</p><p>Conclusions</p><p>Our results indicated that severe sepsis and septic shock were common complications in ICU patients and with high mortality in China, and can be of help to know more about severe sepsis and septic shock in China and to improve characterization and risk stratification in these patients.</p></div

Flow diagram of enrolled patients and their outcome.

<p>ICU, intensive care unit; LOS, length of stay.</p

Distribution of microorganisms isolated from 148 patients.

<p>*including Burkholderia cepacia, Chryseobacter iumindologenes, Enterobacter cloacae, Enterobacteraerogenes, and Serratialiquefaciens.</p><p>**fungal infection here refers to the invasive fungal infection and fungemia.</p><p>Distribution of microorganisms isolated from 148 patients.</p

Characteristics and outcome of patients with severe sepsis.

<p>APACHE II, Acute Physiology and Chronic Health Evaluation II; ARDS, acute respiratory distress syndrome; COPD, Chronic obstructive pulmonary disease; ICU, Intensive care unit; IQR, interquartile range; SOFA, Sequential Organ Failure Assessment.</p><p>Characteristics and outcome of patients with severe sepsis.</p

Characteristics of participating ICUs.

<p>ICU, intensive care unit; IQR, interquartile range.</p><p>Characteristics of participating ICUs.</p

The source of infection of patients with severe sepsis (total >100% because 167 patients had more than one infection sites).

<p>The source of infection of patients with severe sepsis (total >100% because 167 patients had more than one infection sites).</p