Quantification of the influence of drugs on zebrafish larvae swimming kinematics and energetics

The use of zebrafish larvae has aroused wide interest in the medical field for its potential role in the development of new therapies. The larvae grow extremely quickly and the embryos are nearly transparent which allows easy examination of its internal structures using fluorescent imaging techniques. Medical treatment of zebrafish larvae can directly influence its swimming behaviours. These behaviour changes are related to functional changes of central nervous system and transformations of the zebrafish body such as muscle mechanical power and force variation, which cannot be measured directly by pure experiment observation. To quantify the influence of drugs on zebrafish larvae swimming behaviours and energetics, we have developed a novel methodology to exploit intravital changes based on observed zebrafish locomotion. Specifically, by using an in-house MATLAB code to process the recorded live zebrafish swimming video, the kinematic locomotion equation of a 3D zebrafish larvae was obtained, and a customised Computational Fluid Dynamics tool was used to solve the fluid flow around the fish model which was geometrically the same as experimentally tested zebrafish. The developed methodology was firstly verified against experiment, and further applied to quantify the fish internal body force, torque and power consumption associated with a group of normal zebrafish larvae vs. those immersed in acetic acid and two neuroactive drugs. As indicated by our results, zebrafish larvae immersed in 0.01% acetic acid display approximately 30% higher hydrodynamic power and 10% higher cost of transport than control group. In addition, 500 μM diphenylhydantoin significantly decreases the locomotion activity for approximately 50% lower hydrodynamic power, whereas 100 mg/L yohimbine has not caused any significant influences on 5 dpf zebrafish larvae locomotion. The approach has potential to evaluate the influence of drugs on the aquatic animal’s behaviour changes and thus support the development of new analgesic and neuroactive drugs

A contrast-sensitive reversible visible image watermarking technique

A reversible (also called lossless, distortion-free, or invertible) visible watermarking scheme is proposed to satisfy the applications, in which the visible watermark is expected to combat copyright piracy but can be removed to losslessly recover the original image. We transparently reveal the watermark image by overlapping it on a user-specified region of the host image through adaptively adjusting the pixel values beneath the watermark, depending on the human visual system-based scaling factors. In order to achieve reversibility, a reconstruction/ recovery packet, which is utilized to restore the watermarked area, is reversibly inserted into non-visibly-watermarked region. The packet is established according to the difference image between the original image and its approximate version instead of its visibly watermarked version so as to alleviate its overhead. For the generation of the approximation, we develop a simple prediction technique that makes use of the unaltered neighboring pixels as auxiliary information. The recovery packet is uniquely encoded before hiding so that the original watermark pattern can be reconstructed based on the encoded packet. In this way, the image recovery process is carried out without needing the availability of the watermark. In addition, our method adopts data compression for further reduction in the recovery packet size and improvement in embedding capacity. The experimental results demonstrate the superiority of the proposed scheme compared to the existing methods