Extension of summer (June–August) temperature records for northern Inner Mongolia (1715–2008), China using tree rings

This paper presents a spatially and temporally improved reconstruction of mean summer (June–August) temperature derived from tree-ring width data of Dahurian larch (Larix gmelinii Rupr.) from the northern Great Xing'an Mountains, Northeast China. Three new chronologies were added to the original 2011 reconstruction, and the reconstruction extended back to AD 1715. The reconstruction was generated using a simple linear regression method, verified by independent meteorological data, and accounts for 47.0% of the actual temperature variance during the common period (1957–2008). The reconstruction captures decadal and century-scale regional temperature variability, such as cold decades (1940s, 1930s, 1790s, 1950s and 1850s), warm decades (2000s, 1870s, 1750s, 1980s and 1840s), a cold half-century (ca. 1750–1799), and a warm half-century (ca. 1900–1949). It also reveals slightly higher frequency of cold years (20.4%) than warm years (18.0%), and a recent warming trend. Compared to the original 2011 reconstruction, this reconstruction has lower inter-annual temperature variability, high explained variance and high representativeness of regional climate. The reconstruction also correlates with the East Asian Monsoon and the Pacific Ocean signals, and indicates the feasibility of using tree rings from high latitude Northeast China to reconstruct summer temperature in permafrost forest environments

Effect of Nitrogen Addition on Selection of Germination Trait in an Alpine Meadow on the Tibet Plateau

Seed germination requirements may determine the kinds of habitat in which plants can survive. We tested the hypothesis that nitrogen (N) addition can change seed germination trait-environmental filter interactions and ultimately redistribute seed germination traits in alpine meadows. We determined the role of N addition on germination trait selection in an alpine meadow after N addition by combining a 3-year N addition experiment in an alpine meadow and laboratory germination experiments. At the species level, germination percentage, germination rate (speed) and breadth of temperature niche for germination (BTN) were positively related to survival of a species in the fertilized community. In addition, community-weighted means of germination percentage, germination rate, germination response to alternating temperature and BTN increased. However, germination response to wet-cold storage (cold stratification) and functional richness of germination traits was lower in alpine meadows with high-nitrogen addition than in those with no, low and medium N addition. Thus, N addition had a significant influence on environmental filter-germination trait interactions and generated a different set of germination traits in the alpine meadow. Further, the effect of N addition on germination trait selection by environmental filters was amount-dependent. Low and medium levels of N addition had less effect on redistribution of germination traits than the high level

Study on the evaluation of the clinical effects of traditional chinese medicine in heart failure by complex intervention: protocol of SECETCM-HF

<p>Abstract</p> <p>Background</p> <p>Experts in Traditional Chinese Medicine (TCM) have studied the TCM subject of the pathogenesis of heart failure (HF) for several decades. As a result, the general idea is <it>ben </it>deficiency and <it>biao </it>excess. However, the clinical evaluation system which combined the TCM and western medicine in HF has not been developed yet. The objective is to establish the evaluation index system for the integration of TCM and western medicine. The evaluation indexes which include TCM items will specify the research design and methods.</p> <p>Methods</p> <p>Nine medical centers in different cities in China will participate in the trial. A population of 340 patients with HF will be enrolled through a central randomized system for different test groups. Group A will be treated with only western medicine, while group B with western and Chinese medicine together. The study will last for 12 months from the date of enrollment. The cardiovascular death will be the primary outcome.</p> <p>Discussion</p> <p>By putting the protocol into practice, the clinical effects of TCM for HF will be identified scientifically, objectively as well as rationally. The proper index system which built in the study will be helpful for the clinical effect expression of HF by integrated medicine in future.</p> <p>Trial Registration</p> <p>ChiCTR-TRC-00000059</p

Probing behaviors and their plasticity for the aphid Sitobion avenae on three alternative host plants.

Insects may develop different behavioral phenotypes in response to heterogeneous environments (e.g., host plants), but the plasticity of their feeding behaviors has been rarely explored. In order to address the issue, clones of the English grain aphid, Sitobion avenae (Fabricius), were collected from wheat, and their probing behaviors were recorded on three plants. Our results demonstrated that S. avenae individuals on the alternative plants (i.e., barley and oat) tended to have higher frequency of non-probing (Np), increased duration of the pathway phase, increased phloem salivation, and decreased phloem ingestion (E2), compared to those on the source plant (i.e., wheat), showing the resistance of barley and oat to this aphid's feeding. This aphid showed apparently high extents of plasticity for all test probing behaviors on barley or oat. Positive selection for higher extents of plasticity in E2 duration was identified on barley and oat. The factor 'clone' alone explained 30.6% to 70.1% of the total variance for each behavioral plasticity, suggesting that the divergence of probing behavior plasticity in S. avenae had a genetic basis. This aphid's fitness correlated positively with the plasticity of Np frequency and E2 frequency. Some behaviors and their corresponding plasticities (e.g., the frequency of xylem ingestion and its plasticity) were found to be correlated characters, probably reflecting the limits for the evolution of higher extents of behavioral plasticity in this aphid. The differential probing behaviors and their plasticity in S. avenae can have significant implications for the adaptation and management of aphids on different plants

Adsorption of Heavy Metals on Soil Collected from Lixisol of Typical Karst Areas in the Presence of CaCO3 and Soil Clay and Their Competition Behavior

The content of heavy metals in the soil in Guizhou Province, which is a high-risk area for heavy metal exposure, is significantly higher than that in other areas in China. Therefore, the objective of this study was to evaluate the ability of CaCO3 and clay to accumulate heavy metals in topsoil sample collected from Lixisol using the method of indoor simulation. The results showed that the contents of Cu, Zn, Cd, Cr, Pb, Hg and As in the soil sample were 10.8 mg/kg, 125 mg/kg, 0.489 mg/kg, 23.5 mg/kg, 22.7 mg/kg, 58.3 mg/kg and 45.4 mg/kg, respectively. The soil pH values increased with the CaCO3 concentration in the soil, and the fluctuation of the soil pH values was weak after the CaCO3 concentrations reached 100 g/kg. The adsorption capacity of lime soil increased by approximately 10 mg/kg on average, and the desorption capacity decreased by approximately 300 mg/kg on average. The desorption of all heavy metals in this study did not change with increasing clay content. Pseudo-second-order kinetics were more suitable for describing the adsorption kinetics of heavy metals on the soil material, as evidenced by the higher R2 value. The Freundlich model can better describe the adsorption process of As on lime soil. The process of As, Cr, Cd and Hg adsorption on the soil sample was spontaneous and entropy-driven. Additionally, the process of Cu and Pb adsorption on the soil materials was spontaneous and enthalpy-driven. Generally, the adsorption and desorption of heavy metals in polluted soil increased and decreased, respectively, with increasing CaCO3 content. The effect of calcium carbonate on the accumulation of heavy metals in soil was greater than that of clay. In summary, CaCO3 and pH values in soil can be appropriately added in several areas polluted by heavy metals to enhance the crop yield and reduce the adsorption of heavy metals in soils

Synergistic Effect of Quercetin on Antibacterial Activity of Florfenicol Against Aeromonas hydrophila In Vitro and In Vivo

The overuse or abuse of antimicrobial drugs in aquaculture, aggravates the generation of drug-resistant bacteria, which has caused potential risks to human health and the aquaculture industry. Flavonoid&ndash;antibiotic combinations have been shown to suppress the emergence of resistance in bacteria, and sometimes even reverse it. Here, the antibacterial activity of florfenicol in combination with quercetin, a potential drug to reverse multidrug resistance, was tested against Aeromonas hydrophila (A. hydrophila). Of eleven selected antimicrobial agents, quercetin and florfenicol showed the strongest bactericidal effect, and fractional inhibitory concentration (FIC) indices were 0.28, showing a highly synergistic effect. Then, the antibacterial activities of quercetin and florfenicol against A. hydrophila were further tested in vitro and in vivo. Bacterial viability of A. hydrophila decreased in a florfenicol dose-dependent manner, about 16.3&ndash;191.4-fold lower in the presence of 15 &mu;g/mL quercetin and 0.156 to 1.25 &mu;g/mL florfenicol than in the absence of quercetin, respectively. The cell killing was maximum at 45 &mu;g/mL quercetin in the dose range tested plus 0.156 &mu;g/mL florfenicol. The viability decreased over time during the combined treatment with quercetin and florfenicol by 60.5- and 115-fold in 0.156 &mu;g/mL florfenicol and 0.625 &mu;g/mL florfenicol, respectively. Additionally, the synergistic effect was confirmed by the bacterial growth curve. Furthermore, quercetin and florfenicol had an obvious synergistic activity in vivo, reducing the bacterial load in the liver, spleen and kidney tissues of Cyprinus carpio up to 610.6-fold compared with the florfenicol group, and improving the survival rate of infected fish from 10% in the control group to 90% in drug combinations group. These findings indicated that quercetin could potentiate the antibacterial activity of florfenicol against A. hydrophila infection and may reduce the use of antimicrobial drugs and improve the prevention and control capability of bacterial resistance

Extension of summer (June–August) temperature records for northern Inner Mongolia (1715–2008), China using tree rings: Supplementary data

Supplementary data for a paper that presents a spatially and temporally improved reconstruction of mean summer (June–August) temperature derived from tree-ring width data of Dahurian larch (Larix gmelinii Rupr.) from the northern Great Xing'an Mountains, Northeast China. Three new chronologies were added to the original 2011 reconstruction, and the reconstruction extended back to AD 1715. The reconstruction was generated using a simple linear regression method, verified by independent meteorological data, and accounts for 47.0% of the actual temperature variance during the common period (1957–2008). The reconstruction captures decadal and century-scale regional temperature variability, such as cold decades (1940s, 1930s, 1790s, 1950s and 1850s), warm decades (2000s, 1870s, 1750s, 1980s and 1840s), a cold half-century (ca. 1750–1799), and a warm half-century (ca. 1900–1949). It also reveals slightly higher frequency of cold years (20.4%) than warm years (18.0%), and a recent warming trend. Compared to the original 2011 reconstruction, this reconstruction has lower inter-annual temperature variability, high explained variance and high representativeness of regional climate. The reconstruction also correlates with the East Asian Monsoon and the Pacific Ocean signals, and indicates the feasibility of using tree rings from high latitude Northeast China to reconstruct summer temperature in permafrost forest environments

A New Deep Learning Algorithm for SAR Scene Classification Based on Spatial Statistical Modeling and Features Re-Calibration

Synthetic Aperture Radar (SAR) scene classification is challenging but widely applied, in which deep learning can play a pivotal role because of its hierarchical feature learning ability. In the paper, we propose a new scene classification framework, named Feature Recalibration Network with Multi-scale Spatial Features (FRN-MSF), to achieve high accuracy in SAR-based scene classification. First, a Multi-Scale Omnidirectional Gaussian Derivative Filter (MSOGDF) is constructed. Then, Multi-scale Spatial Features (MSF) of SAR scenes are generated by weighting MSOGDF, a Gray Level Gradient Co-occurrence Matrix (GLGCM) and Gabor transformation. These features were processed by the Feature Recalibration Network (FRN) to learn high-level features. In the network, the Depthwise Separable Convolution (DSC), Squeeze-and-Excitation (SE) Block and Convolution Neural Network (CNN) are integrated. Finally, these learned features will be classified by the Softmax function. Eleven types of SAR scenes obtained from four systems combining different bands and resolutions were trained and tested, and a mean accuracy of 98.18% was obtained. To validate the generality of FRN-MSF, five types of SAR scenes sampled from two additional large-scale Gaofen-3 and TerraSAR-X images were evaluated for classification. The mean accuracy of the five types reached 94.56%; while the mean accuracy for the same five types of the former tested 11 types of scene was 96%. The high accuracy indicates that the FRN-MSF is promising for SAR scene classification without losing generality