Metabolizable energy requirement for maintenance estimated by regression analysis of body weight gain or metabolizable energy intake in growing pigs

Objective: Feed energy required for pigs is first prioritized to meet maintenance costs. Additional energy intake in excess of the energy requirement for maintenance is retained as protein and fat in the body, leading to weight gain. The objective of this study was to estimate the metabolizable energy requirements for maintenance (MEm) by regressing body weight (BW) gain against metabolizable energy intake (MEI) in growing pigs.Methods: Thirty-six growing pigs (26.3 +/- 1.7 kg) were allotted to 1 of 6 treatments with 6 replicates per treatment in a randomized complete block design. Treatments were 6 feeding levels which were calculated as 50%, 60%, 70%, 80%, 90%, or 100% of the estimated ad libitum MEI (2,400 kJ/kg BW0.60 d). All pigs were individually housed in metabolism crates for 30 d and weighed every 5 d. Moreover, each pig from each treatment was placed in the open-circuit respiration chambers to measure heat production (HP) and energy retained as protein (REp) and fat (REf) every 5 d. Serum biochemical parameters of pigs were analyzed at the end of the experiment.Results: The average daily gain (ADG) and HP as well as the REp and REf linearly increased with increasing feed intake (p< 0.010). beta-hydroxybutyrate concentration of serum tended to increase with increasing feed intake (p = 0.080). The regression equations of MEI on ADG were MEI, kJ/kg BW0.60 d = 1.88xADG, g/d+782 (R-2 = 0.86) and MEm was estimated at 782 kJ/kg BW0.60 d. Protein retention of growing pigs would be positive while REf would be negative at this feeding level via regression equations of REp and REf on MEI.Conclusion: The MEm was estimated at 782 kJ/kg BW0.60 d in current experiment. Furthermore, growing pigs will deposit protein and oxidize fat if provided feed at the estimated maintenance level

Mixed organic acids improve nutrients digestibility, volatile fatty acids composition and intestinal microbiota in growing-finishing pigs fed high-fiber diet

Objective The objective of this study was to investigate effects of mixed organic acids (MOA) on nutrient digestibility, volatile fatty acids composition and intestinal microbiota in growing-finishing pigs fed high wheat bran diet. Methods Six crossbred barrows (Duroc×Landrace×Yorkshire), with an average body weight 78.8±4.21 kg, fitted with T-cannulas at the distal ileum, were allotted to a double 3×3 Latin square design with 3 periods and 3 diets. Each period consisted of a 5-d adjustment period followed by a 2-d total collection of feces and then a 2-d collection of ileal digesta. The dietary treatments included a corn-soybean-wheat bran basal diet (CTR), mixed organic acid 1 diet (MOA1; CTR+3,000 mg/kg OA1), mixed organic acid 2 diet (MOA2; CTR+2,000 mg/kg OA2). Results Pigs fed MOA (MOA1 or MOA2) showed improved (p<0.05) apparent total tract digestibility (ATTD) of gross energy, dry matter and organic matter, and pigs fed MOA2 had increased (p<0.05) ATTD of neutral detergent fiber compared to CTR. Dietary MOA supplementation decreased (p<0.05) pH value, and improved (p<0.01) concentrations of lactic acid and total volatile fatty acids (TVFA) in ileum compared to CTR. Pigs fed MOA showed higher (p<0.05) concentration of acetic acid, and lower (p<0.05) content of formic acid in feces compared to CTR. Pigs fed MOA1 had increased (p<0.05) concentration of TVFA and butyric acid in feces. Pigs fed MOA1 showed higher concentration of Lactobacillus and lower concentration of Escherichia in feces compared to CTR. Conclusion Dietary supplementation of MOA 1 or 2 could improve nutrients digestibility, TVFA concentration and intestinal flora in growing-finishing pigs fed high fiber diet

The Effect of Inclusion Level of Soybean Oil and Palm Oil on Their Digestible and Metabolizable Energy Content Determined with the Difference and Regression Method When Fed to Growing Pigs

This experiment was conducted to determine the effects of inclusion level of soybean oil (SO) and palm oil (PO) on their digestible and metabolism energy (DE and ME) contents when fed to growing pigs by difference and regression method. Sixty-six crossbred growing barrows (Duroc×Landrace×Yorkshire and weighing 38.1±2.4 kg) were randomly allotted to a 2×5 factorial arrangement involving 2 lipid sources (SO and PO), and 5 levels of lipid (2%, 4%, 6%, 8%, and 10%) as well as a basal diet composed of corn and soybean meal. The barrows were housed in individual metabolism crates to facilitate separate collection of feces and urine, and were fed the assigned test diets at 4% of initial body weight per day. A 5-d total collection of feces and urine followed a 7-d diet adaptation period. The results showed that the DE and ME contents of SO and PO determined by the difference method were not affected by inclusion level. The DE and ME determined by the regression method for SO were greater compared with the corresponding respective values for PO (DE: 37.07, ME: 36.79 MJ/kg for SO; DE: 34.11, ME: 33.84 MJ/kg for PO, respectively). These values were close to the DE and ME values determined by the difference method at the 10% inclusion level (DE: 37.31, ME: 36.83 MJ/kg for SO; DE: 34.62, ME: 33.47 MJ/kg for PO, respectively). A similar response for the apparent total tract digestibility of acid-hydrolyzed ether extract (AEE) in lipids was observed. The true total tract digestibility of AEE in SO was significantly (p<0.05) greater than that for PO (97.5% and 91.1%, respectively). In conclusion, the DE and ME contents of lipid was not affected by its inclusion level. The difference method can substitute the regression method to determine the DE and ME contents in lipids when the inclusion level is 10%

Essential Oil Blend Could Decrease Diarrhea Prevalence by Improving Antioxidative Capability for Weaned Pigs

Finding an alternative to in-feed antibiotics is important because of increasing contemporary concern regarding drug residues and the development of drug-resistant bacteria. The purpose of this study was to test the hypothesis that essential oils added to the feed would decrease diarrhea prevalence in post-weaned pigs. Ninety weaned piglets (initial body weight (BW): 8.1 &plusmn; 1.4 kg) were randomly assigned to one of three dietary diets: (1) a control diet (CON, the basal diet without antibiotics), (2) an antibiotic diet (AB, CON supplemented with colistin sulfate, 20 mg/kg and bacitracin zinc, 40 mg/kg), or (3) an essential oil diet (EO, CON supplemented with an essential oil blend 100 mg/kg) in a completely randomized block design for a 28-day period. The results revealed that AB and EO improved the average daily gain of the piglets from day (d) 15 to 28 (p &lt; 0.05). The diarrhea prevalence in piglets fed AB and EO was lower than that of piglets fed CON (p &lt; 0.05). There was no significant difference in the growth performance or diarrhea prevalence between the AB and EO treatments. Nutrient digestibility was measured at d 28. Compared with CON, EO increased the apparent total tract digestibility of gross energy and crude protein (p &lt; 0.05). Villus height in the duodenum and the ratio of villus height to crypt depth in the jejunum for piglets fed AB and EO was greater than those for piglets fed CON (p &lt; 0.05). The essential oil blend improved the superoxide dismutase (SOD) and catalase (CAT) activities and total antioxidant capacity (T-AOC), but decreased the 8-hydroxy deoxyguanosine content in serum on d 14 (p &lt; 0.05). Decreased malondialdehyde (MDA) and protein carbonyl content were observed on d 28 in comparison with CON (p &lt; 0.05). The mucosa in the jejunum of pigs fed EO had greater T-AOC, SOD levels, and glutathione peroxidase (GSH-Px) activities than that of pigs fed CON (p &lt; 0.05). Pigs fed EO and AB had greater GSH-Px activity in the liver tissue than pigs fed CON (p &lt; 0.05). Not only did jejunal and ileal mucosa have EO upregulated SOD1 mRNA expression (p &lt; 0.05), this was also the case in liver tissue. GPx1 expression in the ileal mucosa and GPx4 expression in the liver tissue were higher for pigs fed EO when compared to those fed CON (p &lt; 0.05). Collectively, a dietary essential oil blend supplementation, which has natural antimicrobial properties, could enhance growth performance and decrease diarrhea prevalence in weaned pigs through increases in antioxidative capacity

Validation of metabolisable energy prediction equation for de-oiled corn distillers dried grains with solubles fed to finishing pigs

This experiment was conducted to determine the accuracy of a prediction equation for metabolisable energy (ME) content generated from the chemical composition of de-oiled corn distillers dried grains with solubles (DDGS) in finishing pigs. A total of 72 pigs (average initial body weight 85.7 kg), with four pigs per pen and six pens per treatment, were fed one of the three experimental diets. The experimental diets were a corn-soybean meal basal diet or two diets containing 30% de-oiled DDGS diets. The experimental diets were formulated using ME values obtained from a prediction equation or NRC. ME prediction equation for the de-oiled DDGS was ME = 4066 – (46.3×%neutral detergent fibre (NDF)) + (45.8×%CP) – (106.2×%ash). Overall, the pigs fed the diet calculated with an ME from NRC had significantly lower ADG, feed efficiency, hot carcass weight, Longissimus muscle area and leptin concentration in serum compared with pigs fed the diet with an ME calculated by the prediction equation (p < 0.05). Glucose and low-density lipoprotein concentrations in pigs fed the diet calculated by NRC and were significantly reduced compared with pigs fed the control diet (p < 0.05). Lipase concentration in pigs fed the diet calculated by prediction equation was higher compared with pigs fed the diet calculated by NRC (p < 0.05). In conclusion, the prediction equation of ME content in de-oiled DDGS was more accurate than the value and its use resulted in improved pig performance

Effects of lysozyme on the growth performance, nutrient digestibility, intestinal barrier and microbiota of weaned pigs fed diets containing spray-dried whole egg or albumen powder

Two experiments were conducted to evaluate energy value of spray-dried whole egg (SWE), albumen powder (AP) and effects of lysozyme in diets containing these two protein sources. Exp. 1: 18 barrows were allotted to 1 of 3 diets (Basal, SWE and AP). Both digestible (DE) and metabolizable energy (ME) were lower (PThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Effects of proteinate complex zinc on growth performance, hepatic and splenic trace elements concentrations, antioxidative function and immune functions in weaned piglets

Objective To assess the effects of proteinate complex zinc (PC-Zn) on growth performance, antioxidative function, trace element concentrations and immune function in weaned piglets. Methods Three hundred newly weaned barrows (Duroc×Landrace×Yorkshire), 28 days of age, were randomly allotted to 3 dietary groups of 5 replicate pens per group for 4 weeks of feeding. Experimental diets were: i) zinc deficient diet (ZnD, 24 mg/kg Zn supplementation from ZnSO4), ii) inorganic Zn diet supplemented with 120 mg/kg of Zn from Zn sulfate (ZnSO4), and iii) organic Zn diet supplemented with 120 mg/kg of Zn from PC-Zn. The body weight of pigs were recorded at the beginning, at the middle and at the end of the experiment, and the amount of feed supplied each day was recorded. Five barrows from each dietary treatment group were selected to be anesthetized and euthanized at the end of the trial to determine the Zn, Cu, Fe, and Mn concentrations, the hepatic metallothionein content, the levels of methane dicarboxylic aldehyde (MDA), Mn, and Cu/Zn superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in the spleen, the levels of interleukin (IL)-2, IL-4, IL-10, interferon (IFN)-γ, CD3+, CD4+, and CD8+ T lymphocyte. Results The accumulation of Zn in the spleen, levels of SOD, GSH-Px, IL-4, IL-10, the proportions of CD3+ and CD4+ T lymphocyte, and the ratio of CD4+/CD8+ T lymphocyte were increased by organic Zn supplementation compared to ZnD, while the levels of MDA, IFN-γ, and proportion of CD8+ T lymphocyte were lowered. Conclusion These findings indicate that Zn can improve the antioxidant potential and immune functions of weaned piglets

The Impact of Wheat Bran on the Morphology and Physiology of the Gastrointestinal Tract in Broiler Chickens

There is increasing evidence showing that moderate amounts of insoluble dietary fiber can improve nutrient utilization by positively influencing the physiology of the gastrointestinal tract. The present study was conducted to investigate the effects of wheat bran as a source of insoluble fiber on nutrient digestibility, serum antioxidant status, gastrointestinal development, digestive enzyme activities and intestinal morphology in broiler chickens. A total of 96 one-day-old male Arbor Acre broiler chickens were assigned to two treatments with six replicate cages per treatment and eight birds per replicate for 42 d. Dietary treatments consisted of the control group (CON, control diet) and wheat bran group (WB, 30 g/kg wheat bran). Inclusion of WB increased (p &lt; 0.05) the digestibility of dry matter, organic matter, gross energy and crude protein on Day 42. Birds fed WB had lower (p &lt; 0.05) serum total cholesterol concentration on Day 21, and lower (p &lt; 0.05) serum concentrations of low-density lipoprotein, total cholesterol and total triglyceride on Day 42. Inclusion of WB increased (p &lt; 0.05) serum glutathione peroxidase activity on Day 21 and superoxide dismutase activity on Day 42, but tended (p = 0.07) to decrease serum malondialdehyde concentration on Day 21, and significantly decreased (p &lt; 0.05) serum malondialdehyde concentration on Day 42. Birds fed WB had a greater (p &lt; 0.05) relative weight of gizzard on both Day 21 and 42. Inclusion of WB increased (p &lt; 0.05) activities of amylase and trypsin in pancreas and jejunal mucosa on Day 21, and increased (p &lt; 0.05) amylase activity in pancreas and jejunal mucosa. Birds fed WB had greater (p &lt; 0.05) villus height and villus height to crypt depth ratio in jejunum and ileum on Day 42. In conclusion, supplementation of 30 g/kg WB enhanced nutrient digestibility by improving antioxidant status, gizzard development, intestinal digestive enzyme activities and morphology of broilers

Effects of Forsythia Suspense Extract as an Antibiotics Substitute on Growth Performance, Nutrient Digestibility, Serum Antioxidant Capacity, Fecal Escherichia coli Concentration and Intestinal Morphology of Weaned Piglets

The aim of this study is to determine the efficiency of Forsythia suspense extract (FSE) as an antibiotics substitute on performance, nutrient digestibility, serum antioxidant capacity, fecal Escherichia coli concentration and intestinal morphology of weaned piglets. A total of 108 Duroc &times; (Landrace &times; Yorkshire) weaned piglets (28 days (d) weaned, average body weight of 8.68 &plusmn; 1.36 kg) were randomly assigned into three dietary treatments, six pens per treatment, three barrows and three gilts per pen. The treatments contained a corn-soybean meal basal diet (CTR), an antibiotic diet (basal diet + 75 mg/kg chlortetracycline; CTC), and an FSE diet (basal diet + 200 mg/kg FSE; FSE). The experiment included phase 1 (d 1 to 14), phase 2 (d 15 to 28) and phase 3 (d 29 to 35). Compared with CTR, piglets fed FSE show improved (p &lt; 0.05) average daily gain (ADG) and average daily feed intake in phase 2, as well as enhanced (p &lt; 0.05) ADG from day 15 to 35 and day 1 to 28. Piglets supplemented with CTC and FSE showed a reduced (p &lt; 0.05) diarrhea rate in phase 1, while piglets fed FSE showed enhanced (p &lt; 0.05) apparent total tract digestibility (ATTD) of dry matter, organic matter, crude protein and gross energy, as well as lower (p &lt; 0.05) nitrogen output in phase 2 compared with CTR and CTC. The content in the form of Colony-Forming Units (CFUs) of fecal E. coli on day 14 and 28 was lower (p &lt; 0.05) in piglets fed FSE in comparison with CTR. The contents of total antioxidant capacity, superoxide dismutase and catalase in serum are enhanced (p &lt; 0.05) compared with CTR and CTC, whereas the concentration of malondialdehyde in serum was decreased (p &lt; 0.05) for piglets fed FSE on day 28 compared with CTC. The villus height to crypt depth ratio in ileum was numerically higher (p &lt; 0.05) in piglets fed FSE in comparison with CTR. In conclusion, dietary FSE supplementation could substitute CTC in improving antioxidant capacity, nutrients digestibility and reducing fecal E. coli content, so as to reduce nitrogen output and diarrhea rate, and eventually improve performance in weaned piglets