Case report: Primary pleural low-grade fibromyxoid sarcoma in a 4-year-old boy with molecular confirmation

Low-grade fibromyxoid sarcoma (LGFMS) is a rare malignant fibroblastic tumor, principally affecting the deep tissues of the proximal trunk and extremities in young adults. However, primary pleural LGFMS is extremely rare, and only three cases have been reported in the previous English literature without genetic confirmation. Furthermore, the historical pleural LGFMS cases were all adults, and the primary pleural LGFMS in children has never been reported to date. Here, we presented a primary pleural LGFMS in a 4-year-old boy with detailed clinical, pathological, and molecular results. Histologically, the current tumor showed typical alternating collagenous and myxoid areas, containing spindled or oval tumor cells arranged in a whorled and short fascicular pattern. In some areas, the tumor cells exhibited moderate atypia, and mitotic figures were identified but without the identification of giant collagen rosettes. Immunohistochemically, all the neoplastic cells showed strong and diffuse positivity for MUC4. Genetically, FUS gene rearrangement was revealed by fluorescence in-situ hybridization (FISH), and subsequently, next-generation sequencing (NGS) and polymerase chain reaction (PCR) further demonstrated the FUS::CREB3L2 fusion transcript. To the best of our knowledge, this is the first case of primary pleural LGFMS with the identification of FUS gene rearrangement and FUS::CREB3L2 fusion in a 4-year-old child. Our study expands the age range of pleural LGFMS and highlights the combination of morphological, immunohistochemical, and molecular analyses in such challenging cases

Predicting the Potential Habitat of Three Endangered Species of Carpinus Genus under Climate Change and Human Activity

The impact of climate change and human activities on endangered plants has been a serious concern in forest ecology. Some Carpinus plants have become extinct. Thus, we need to pay more attention to the Carpinus plants that are not yet extinct but are endangered. Here, we employed the species distribution model (SDM) considering different climate change scenarios and human footprint to test the potential habitat changes of three Carpinus species (C. oblongifolia, C. tientaiensis, and C. purpurinervis) in the future. Our results showed that the mean diurnal range of temperature (MDRT), isothermality, mean temperature of wettest quarter, and human footprint were the most influential factors determining the distribution of C. oblongifolia. Precipitation seasonality (coefficient of variation), MDRT, and precipitation of driest quarter were the most important climatic factors affecting C. tientaiensis. The minimum temperature of the coldest month was the most important factor in the distribution of C. purpurinervis. Our results also showed that the three species had different adaptability and habitat change trends under the future climate change scenarios, although they belong to the same genus. The potential habitats of C. oblongifolia would expand in the future, while the potential habitats of C. tientaiensis and C. purpurinervis would decrease for the same period. The predicted changes of these three endangered species on temporal and spatial patterns could provide a theoretical basis for their conservation strategies.Forestry, Faculty ofNon UBCForest and Conservation Sciences, Department ofReviewedFacult