Investigation of Detonative Combustion Characteristics

AbstractThe pressure and deflagration-to-detonation transition (DDT) characteristics of acetylene and oxygen flame were studied in a detonation tube. The pressure history and the flame velocity along the tube were measured with high frequency pressure transducers and ion probes. By analyzing the data recorded in the experiment, the detonation wave pressure, post-wave pressure and DDT distance were obtained, together with the effects of the initial pressure varying from 2 104 Pa to 105 Pa, equivalence ration from 0.3 to 1.0, and mixture concentration from 60% to 100%. It was found that the detonation pressure was decreased respectively with the decrease of initial pressure, equivalence ratio and mixture concentration, but the DDT distance was enlarged. The DDT distance was found particularly sensitive to mixture concentration

Pulsars in FIRST Observations

We identified 16 pulsars from the Faint Images of the Radio Sky at Twenty-cm (FIRST) at 1.4 GHz. Their positions and total flux densities are extracted from the FIRST catalog. Comparing the source positions with those in the PSRcatalog, we obtained better determined positions of PSRs J1022+1001, J1518+4904, J1652+2651, and proper motion upper limits of another three pulsars PSRs J0751+1807, J1012+5307, J1640+2224. Proper motions of the other 10 pulsars are consistent with the values in the catalog.Comment: 6 pages, 2 figures, 3 tables, submited to CJA

A Unified Framework for Mutual Improvement of SLAM and Semantic Segmentation

This paper presents a novel framework for simultaneously implementing localization and segmentation, which are two of the most important vision-based tasks for robotics. While the goals and techniques used for them were considered to be different previously, we show that by making use of the intermediate results of the two modules, their performance can be enhanced at the same time. Our framework is able to handle both the instantaneous motion and long-term changes of instances in localization with the help of the segmentation result, which also benefits from the refined 3D pose information. We conduct experiments on various datasets, and prove that our framework works effectively on improving the precision and robustness of the two tasks and outperforms existing localization and segmentation algorithms.Comment: 7 pages, 5 figures.This work has been accepted by ICRA 2019. The demo video can be found at https://youtu.be/Bkt53dAehj

Efficient inhibition of hepatitis B virus replication by hepatitis delta virus ribozymes delivered by targeting retrovirus

<p>Abstract</p> <p>Background</p> <p>Hepatitis delta virus (HDV) ribozyme is an attractive molecular tool that can specifically recognize and catalyze the self-cleavage of the viral RNA phosphodiester backbone. However, a major obstacle in the medical application of the HDV ribozyme is the lack of specificity in the delivery of the ribozyme to defined target cells.</p> <p>Results</p> <p>The objective of this study was to determine whether retroviral vectors can deliver the HDV ribozyme into the target cells and to elucidate whether HDV ribozyme plays a role in hepatitis B virus (HBV) replication. In our study, the transduction of helper-free pseudotyped retrovirus, which showed a broad host range, in human hepatoma cells was performed under 2 conditions, that is, in the presence of polymerized human serum albumin (pHSA) and in the absence of pHSA. The transduction ability in the presence of pHSA was higher than in the absence of pHSA. Moreover, HBsAg and HBeAg levels after transductions with pHSA were significantly lower than those in the absence of pHSA, thus indicating that the recombinant retrovirus had HBV-specific cleavage activity and targeted HepG2215 cells.</p> <p>Conclusions</p> <p>These data suggest that this system provides a new approach for targeting hepatocytes and has a great potential in gene therapy for HBV infection.</p

Engineering Haloferax mediterranei as an Efficient Platform for High Level Production of Lycopene

Lycopene attracts increasing interests in the pharmaceutical, food, and cosmetic industries due to its anti-oxidative and anti-cancer properties. Compared with other lycopene production methods, such as chemical synthesis or direct extraction from plants, the biosynthesis approach using microbes is more economical and sustainable. In this work, we engineered Haloferax mediterranei, a halophilic archaeon, as a new lycopene producer. H. mediterranei has the de novo synthetic pathway for lycopene but cannot accumulate this compound. To address this issue, we reinforced the lycopene synthesis pathway, blocked its flux to other carotenoids and disrupted its competitive pathways. The reaction from geranylgeranyl-PP to phytoene catalyzed by phytoene synthase (CrtB) was identified as the rate-limiting step in H. mediterranei. Insertion of a strong promoter PphaR immediately upstream of the crtB gene, or overexpression of the heterologous CrtB and phytoene desaturase (CrtI) led to a higher yield of lycopene. In addition, blocking bacterioruberin biosynthesis increased the purity and yield of lycopene. Knock-out of the key genes, responsible for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) biosynthesis, diverted more carbon flux into lycopene synthesis, and thus further enhanced lycopene production. The metabolic engineered H. mediterranei strain produced lycopene at 119.25 ± 0.55 mg per gram of dry cell weight in shake flask fermentation. The obtained yield was superior compared to the lycopene production observed in most of the engineered Escherichia coli or yeast even when they were cultivated in pilot scale bioreactors. Collectively, this work offers insights into the mechanism involved in carotenoid biosynthesis in haloarchaea and demonstrates the potential of using haloarchaea for the production of lycopene or other carotenoids