Calculation of Carbon Emissions and Study of the Emission Reduction Path of Conventional Public Transportation in Harbin City

As the northernmost megacity in China, the long winters, large population size, and unsustainable transport structure in Harbin determine that the city will produce relatively large carbon emissions. The transportation industry is one of the three greenhouse gas emission sources; therefore, the development of low-carbon transportation is imperative. This work compares commonly used carbon emission measurement methods and chooses a mileage method to classify the carbon emissions of conventional buses of different energy types used in Harbin in 2020. A multi-factor grey prediction model was constructed to predict the population size of Harbin and the number of conventional buses. After that, a scenario analysis method was used to analyze the fuel structure of buses in Harbin from three perspectives: a pessimistic scenario, a baseline scenario, and an optimistic scenario. The carbon emissions of conventional buses were calculated for Harbin from 2023 to 2030. Finally, by combining the prediction results and factors influencing carbon emission, a regular bus path to minimize carbon emissions is proposed. The outcome of this study shows that the carbon emission environment in Harbin will be improved by reducing vehicle energy consumption, optimizing energy structure, standardizing driving behavior, building intelligent transportation, giving priority to public transportation, and improving the road network structure

Genomic and transcriptomic analysis of the endophytic fungus Pestalotiopsis fici reveals its lifestyle and high potential for synthesis of natural products.

BackgroundIn recent years, the genus Pestalotiopsis is receiving increasing attention, not only because of its economic impact as a plant pathogen but also as a commonly isolated endophyte which is an important source of bioactive natural products. Pestalotiopsis fici Steyaert W106-1/CGMCC3.15140 as an endophyte of tea produces numerous novel secondary metabolites, including chloropupukeananin, a derivative of chlorinated pupukeanane that is first discovered in fungi. Some of them might be important as the drug leads for future pharmaceutics.ResultsHere, we report the genome sequence of the endophytic fungus of tea Pestalotiopsis fici W106-1/CGMCC3.15140. The abundant carbohydrate-active enzymes especially significantly expanding pectinases allow the fungus to utilize the limited intercellular nutrients within the host plants, suggesting adaptation of the fungus to endophytic lifestyle. The P. fici genome encodes a rich set of secondary metabolite synthesis genes, including 27 polyketide synthases (PKSs), 12 non-ribosomal peptide synthases (NRPSs), five dimethylallyl tryptophan synthases, four putative PKS-like enzymes, 15 putative NRPS-like enzymes, 15 terpenoid synthases, seven terpenoid cyclases, seven fatty-acid synthases, and five hybrids of PKS-NRPS. The majority of these core enzymes distributed into 74 secondary metabolite clusters. The putative Diels-Alderase genes have undergone expansion.ConclusionThe significant expansion of pectinase encoding genes provides essential insights in the life strategy of endophytes, and richness of gene clusters for secondary metabolites reveals high potential of natural products of endophytic fungi

Genetic architecture of the inflammatory bowel diseases across East Asian and European ancestries

Inflammatory bowel diseases (IBDs) are chronic disorders of the gastrointestinal tract with the following two subtypes: Crohn’s disease (CD) and ulcerative colitis (UC). To date, most IBD genetic associations were derived from individuals of European (EUR) ancestries. Here we report the largest IBD study of individuals of East Asian (EAS) ancestries, including 14,393 cases and 15,456 controls. We found 80 IBD loci in EAS alone and 320 when meta-analyzed with ~370,000 EUR individuals (~30,000 cases), among which 81 are new. EAS-enriched coding variants implicate many new IBD genes, including ADAP1 and GIT2. Although IBD genetic effects are generally consistent across ancestries, genetics underlying CD appears more ancestry dependent than UC, driven by allele frequency (NOD2) and effect (TNFSF15). We extended the IBD polygenic risk score (PRS) by incorporating both ancestries, greatly improving its accuracy and highlighting the importance of diversity for the equitable deployment of PRS.</p