Comparative analysis of phosphoproteomic in the intestine of Sepia lycidas under different salinity environments

Cuttlefish are sensitive to the breeding environment, and the low-salinity environment significantly impacts their growth and immunity. So far, it is difficult to breed this species artificially. This study was conducted in Sepia lycidas. And the aim was to investigate the differences in protein phosphorylation in the intestine of S. lycidas under different salinity conditions. Firstly, 999 phosphoproteins (specific peptide ≥ 1), 1928 phosphopeptides, and 2727 phosphorylation sites were identified. Among them were 284 down-regulated expression phosphorylation sites (corresponding to 115 phosphoproteins) and 674 up-regulated expression phosphorylation sites (corresponding to 408 phosphoproteins) in the intestine under a low salinity environment compared with that under a natural salinity environment. Next, GO analysis found that more phosphoproteins corresponding to differentially expressed phosphorylation sites were related to anatomical structure development, multicellular organism development, regulation of the cellular process, etc. The molecular functions of these proteins mainly contain protein binding, transferase activity, catalytic activity, and heterocyclic compound binding. And they are mainly involved in the cellular components of intracellular anatomical structure, organelle, and cytoplasm. KEGG enrichment analysis of the differential phosphoproteins suggested that many significantly enriched pathways were related to the phosphatidylinositol signaling system, cell junction (adherens junction and tight junction), and inositol phosphate metabolism. Finally, changes in environmental salinity can affect the intestinal structure, metabolism, and immune homeostasis of S. lycidas

LLaSM: Large Language and Speech Model

Multi-modal large language models have garnered significant interest recently. Though, most of the works focus on vision-language multi-modal models providing strong capabilities in following vision-and-language instructions. However, we claim that speech is also an important modality through which humans interact with the world. Hence, it is crucial for a general-purpose assistant to be able to follow multi-modal speech-and-language instructions. In this work, we propose Large Language and Speech Model (LLaSM). LLaSM is an end-to-end trained large multi-modal speech-language model with cross-modal conversational abilities, capable of following speech-and-language instructions. Our early experiments show that LLaSM demonstrates a more convenient and natural way for humans to interact with artificial intelligence. Specifically, we also release a large Speech Instruction Following dataset LLaSM-Audio-Instructions. Code and demo are available at https://github.com/LinkSoul-AI/LLaSM and https://huggingface.co/spaces/LinkSoul/LLaSM. The LLaSM-Audio-Instructions dataset is available at https://huggingface.co/datasets/LinkSoul/LLaSM-Audio-Instructions

Strange Quasar Candidates with Abnormal Astrometric Characteristics from Gaia EDR3 and SDSS (SQUAB-II): Optical Identifications

There are some strange quasars with multiple Gaia detections or observed with abnormal astrometric characteristics, such as with large proper motions or significant astrometric noises. Those strange quasars could be potential candidates of quasar-star pairs, dual quasars (DQs), or lensed quasars (LQs). Searching for both DQs and LQs is of great importance in many fields of astrophysics. Here in this work, we select 143 SDSS spectroscopically confirmed quasars that have multiple Gaia EDR3 detections within 1 arcsec of the SDSS quasar' position. We apply several optical identification methods to classify this sample. We firstly exclude 65 quasar-star pairs via their stellar features including their parallaxes and proper motions, stellar features in the SDSS spectra, or via the colour-colour diagram. Based on the spectral-fitting results, we find 2 DQ candidates, one of which presents a double-peaked [O III] emission line feature and the other shows a broad $H_{\beta}$ velocity offset ($\sim$ 870 $km s^{-1}$) relative to the [O III] $\lambda$5007 line. Via the colour difference method, we further find 56 LQ candidates with similar colours in their multiple images. We also cross-match 143 objects with the HST archive and find 19 targets with archival HST images. Our classification results of those 19 targets are mainly consistent with previous works.Comment: 18 pages, 11figures, accepted for publication in MNRA

Advances in the Application of Metabolomics in Cereal Foods

Cereal foods are the primary source of daily energy, protein, and dietary fiber intake for humans. With the continuous improvement of people's living standards, cereal foods' nutritional value, safety, and food quality have attracted much attention. Cereal metabonomics, as a new and rapidly developing branch of "metabonomics", has made rapid progress in quantifying and analyzing various metabolites. This article reviews the analysis process of metabolomics technology, and its application in the detection of component identification, the identification of flavor substances, the traceability of origin, and the product of new developments in cereal foods, and the development trend of metabonomics in cereal food field has prospected, to provide a reference for the in-depth development and application of cereal foods

NAT10 Maintains OGA mRNA Stability Through ac4C Modification in Regulating Oocyte Maturation

In vitro maturation (IVM) refers to the process of developing immature oocytes into the mature in vitro under the microenvironment analogous to follicle fluid. It is an important technique for patients with polycystic ovary syndrome and, especially, those young patients with the need of fertility preservation. However, as the mechanisms of oocyte maturation have not been fully understood yet, the cultivation efficiency of IVM is not satisfactory. It was confirmed in our previous study that oocyte maturation was impaired after N-acetyltransferase 10 (NAT10) knockdown (KD). In the present study, we further explored the transcriptome alteration of NAT10-depleted oocytes and found that O-GlcNAcase(OGA) was an important target gene for NAT10-mediated ac4C modification in oocyte maturation. NAT10 might regulate OGA stability and expression by suppressing its degradation. To find out whether the influence of NAT10-mediated ac4C on oocyte maturation was mediated by OGA, we further explored the role of OGA in IVM. After knocking down OGA of oocytes, oocyte maturation was inhibited. In addition, as oocytes matured, OGA expression increased and, conversely, O-linked N-acetylglucosamine (O-GlcNAc) level decreased. On the basis of NAT10 KD transcriptome and OGA KD transcriptome data, NAT10-mediated ac4C modification of OGA might play a role through G protein–coupled receptors, molecular transduction, nucleosome DNA binding, and other mechanisms in oocyte maturation. Rsph6a, Gm7788, Gm41780, Trpc7, Gm29036, and Gm47144 were potential downstream genes. In conclusion, NAT10 maintained the stability of OGA transcript by ac4C modification on it, thus positively regulating IVM. Moreover, our study revealed the regulation mechanisms of oocytes maturation and provided reference for improving IVM outcomes. At the same time, the interaction between mRNA ac4C modification and protein O-GlcNAc modification was found for the first time, which enriched the regulation network of oocyte maturation

Minute-Cadence Observations of the LAMOST Fields with the TMTS V. Machine Learning Classification of TMTS Catalogues of Periodic Variable Stars

Periodic variables are always of great scientific interest in astrophysics. Thanks to the rapid advancement of modern large-scale time-domain surveys, the number of reported variable stars has experienced substantial growth for several decades, which significantly deepened our comprehension of stellar structure and binary evolution. The Tsinghua University-Ma Huateng Telescopes for Survey (TMTS) has started to monitor the LAMOST sky areas since 2020, with a cadence of 1 minute. During the period from 2020 to 2022, this survey has resulted in densely sampled light curves for ~ 30,000 variables of the maximum powers in the Lomb-Scargle periodogram above the 5sigma threshold. In this paper, we classified 11,638 variable stars into 6 main types using XGBoost and Random Forest classifiers with accuracies of 98.83% and 98.73%, respectively. Among them, 5301 (45.55%) variables are newly discovered, primarily consisting of Delta Scuti stars, demonstrating the capability of TMTS in searching for short-period variables. We cross-matched the catalogue with Gaia's second Data Release (DR2) and LAMOST's seventh Data Release (DR7) to obtain important physical parameters of the variables. We identified 5504 Delta Scuti stars (including 4876 typical Delta Scuti stars and 628 high-amplitude Delta Scuti stars), 5899 eclipsing binaries (including EA-, EB- and EW-type) and 226 candidates of RS Canum Venaticorum. Leveraging the metal abundance data provided by LAMOST and the Galactic latitude, we discovered 8 candidates of SX Phe stars within the class of "Delta Scuti stars". Moreover, with the help of Gaia color-magnitude diagram, we identified 9 ZZ ceti stars.Comment: 20 pages, 14 figures, accepted to MNRA

New-Onset and Relapsed Membranous Nephropathy post SARS-CoV-2 and COVID-19 Vaccination

Since the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak and COVID-19 vaccination, new-onset and relapsed clinical cases of membranous nephropathy (MN) have been reported. However, their clinical characteristics and pathogenesis remained unclear. In this article, we collected five cases of MN associated with SARS-CoV-2 infection and 37 related to COVID-19 vaccination. Of these five cases, four (4/5, 80%) had acute kidney injury (AKI) at disease onset. Phospholipase A2 receptor (PLA2R) in kidney tissue was negative in three (3/5, 60%) patients, and no deposition of virus particles was measured among all patients. Conventional immunosuppressive drugs could induce disease remission. The underlying pathogenesis included the subepithelial deposition of viral antigens and aberrant immune response. New-onset and relapsed MN after COVID-19 vaccination generally occurred within two weeks after the second dose of vaccine. Almost 27% of patients (10/37) suffered from AKI. In total, 11 of 14 cases showed positive for PLA2R, and 20 of 26 (76.9%) presented with an elevated serum phospholipase A2 receptor antibody (PLA2R-Ab), in which 8 cases exceeded 50 RU/mL. Conventional immunosuppressive medications combined with rituximab were found more beneficial to disease remission for relapsed patients. In contrast, new-onset patients responded to conservative treatment. Overall, most patients (24/37, 64.9%) had a favorable prognosis. Cross immunity and enhanced immune response might contribute to explaining the mechanisms of MN post COVID-19 vaccination

Experimental Investigation of Local Scour Protection for Cylindrical Bridge Piers Using Anti-Scour Collars

Local scour of bridge piers is one of the main threats responsible for bridge damage. Adopting scour countermeasures to protect bridge foundations from scour has become an important issue for the design and maintenance of bridges located in erodible sediment beds. This paper focuses on the protective effect of one active countermeasure named an “anti-scour collar” on local scour around the commonly used cylindrical bridge pier. A cylindrical pier model was set up in a current flume. River sand with a median particle size of 0.324 mm was selected and used as the sediment in the basin. A live-bed scour experimental program was carried out to study the protective effect of an anti-scour collar by comparing the local scour at a cylindrical bridge pier model with and without collar. The effects of three design parameters including collar installation height, collar external diameter and collar protection range, on the scour depth and scour development were investigated parametrically. According to the experimental results, it can be concluded that: the application of an anti-scour collar alleviates the local scour at the pier effectively; and the protection effect decreases with an increase in the collar installation height, but increases with an increase in the collar external diameter and the protection range. Design suggestions for improving the scour protective effect of the anti-scour collar are summarized and of great practical guiding significance to the development of anti-scour collars for bridge piers

Epstein - Barr virus-associated lymphoepithelioma-like gastric carcinoma located on gastric high body: Two case reports

Lymphoepithelioma-like gastric carcinoma (LELGC) is a rare neoplasm of the stomach with an incidence of 1-4% of all gastric cancers. It is characterized by the presence of a lymphoid stroma with cells arranged primarily in micro alveolar, thin trabecular, and primitive tubular patterns or isolated cells. It is one of the histological patterns observed in patients with Epstein - Barr virus (EBV)-associated gastric carcinoma (EBVaGC). In situ hybridization was usually used to confirm the presence of EBV. There are two types of EBVaGC, LELGC, and ordinary type. Approximately, 15-25% of EBVaGC exhibit the LELGC pattern. Here, we described two cases of LELGC and the related literatures were reviewed as well. The two cases were submucosal mass from a 59- or 63-year-old man. We found LELGC has special clinicopathologic features and protein expression profile. This should promote us to make a true diagnosis