Characterization of horse meat: chemical composition of muscle and fatty acid profile of subcutaneous fat

3 páginas, 2 tablas, 1 figura.--Trabajo presentado a las XVI Jornadas sobre Producción Animal AIDA (Zaragoza, 19 al 20 de mayo, 2015).Al Departamento de Desarrollo Económico y Competitividad del Gobierno Vasco (beca predoctoral de X. Belaunzaran) y al Ministerio de Economía y Competitividad y a la UPV/EHU (contrato Ramón y Cajal RYC-2011 a 08593 de N. Aldai) Este trabajo ha sido financiado por la UPV/EHU (EHUA13/29, ZALDITRANS).Peer reviewe

Feeding affected the neutral and polar lipid composition of muscle in foals

2 páginas, 1 figura.-- Trabajo presentado al 63st International Congress of Meat Science and Technology. Session 5 Adaptation and fetal programming (5.39, p. 128). 13-18 agosto 2017, (Cork, Irlanda).Financial support by UPV/EHU (EHUA 13/29) and the Basque Government (IT766-13)Peer Reviewe

Characterization of retail horse-meat in Northern Spain: Chemical composition and fatty acid profile

Trabajo presentado al: 61th ICoMST. (Clermont Ferrand, France, 23 al 28 August, 2015).Peer Reviewe

Effect of slaughter age and feeding system on the neutral and polar lipid composition of horse meat

9 páginas, 3 tablas, 2 figuras.This study was undertaken to provide a thorough analysis of the neutral lipid (NL) and polar lipid (PL) fractions of horse meat that included the content and distribution of acyl and alkenyl moieties in foals under different rearing conditions. Two groups of crossbred horses were studied; the first group was selected from suckling foals produced under grazing conditions and slaughtered at 4 months of age (n=8), and the second group was selected from concentrate-finished foals and slaughtered at 12 months of age (n=7). There were significant differences related to the age and feeding practices of foals which affected the intramuscular (IM) fat content and the fatty acid (FA) composition of NL and PL fractions. Samples from suckling foals were leaner and provided the highest content of methylation products from the plasmalogenic lipids, and total and n-3 polyunsaturated fatty acid (PUFA). By contrast, the meat from concentrate-finished foals had a higher IM fat level resulting in a greater accumulation of 16:0 and total monounsaturated FAs in the NL fraction, whereas the muscle PL fraction retained a similar FA composition between both groups. Linolenic acid was preferentially deposited in the NL fraction, but linoleic acid and the long-chain n-3 and n-6 PUFAs were incorporated into the PL fraction where they served as cell membrane constituents and in eicosanoid formation.X. B. thanks the Department of Economic Development and Competitiveness of the Basque Government for his doctoral fellowship, and N. A. thanks the Spanish Ministry of Economy and Competitiveness and the University of the Basque Country (UPV/EHU) for her contract through the ‘Ramón y Cajal (RYC-2011-08593)’ program. Financial support was provided by the UPV/EHU (EHUA 13/29; ZALDITRANS Project) and the Basque Government (IT766-13). Collaboration and help provided by horse producers is very much appreciated.Peer Reviewe

Caracterización de la carne de caballo comercializada en el Norte de España: composición química y perfil de ácidos grasos

Trabajo presentado al: XXIV Congreso de la Asociación Latinoamericana de Producción Animal. (Puerto Varas, Chile, 9 al 13 de noviembre, 2015).Peer Reviewe

An assessment of the fatty acid composition of horse-meat available at the retail level in northern Spain

9 páginas, 5 tablas, 4 figuras.The objective of the present study was to assess the fatty acid composition of horse-meat available at the retail market in northern Spain. Horse steaks (Longissimus thoracis et lumborum muscle; n = 82) were purchased from butcher-shops and large grocery stores throughout six northern regions of Spain in two different seasons. Fat content differed significantly among regions (1.12 to 2.77%). Samples with higher intramuscular fat content presented the highest percentages of total monounsaturated fatty acids and the lowest contents of dimethylacetal and polyunsaturated fatty acids (PUFA), while the opposite was found in the leanest samples. A high variability was observed in the muscle and subcutaneous n-3 PUFA content. Overall, total n-3 PUFA content ranged between 1.17% and 18.9% in muscle fat and between 1.52% and 27.9% in backfat. Interestingly, almost 5% of surveyed loins from horse carcasses (4 out of 82) contained over 300 mg of linolenic acid per 100 g of meat which could have been marketed as a “source” of n-3 FAs according to Commission Regulation (EU) No 116/2010. (C) 2016 Elsevier Ltd. All rights reserved.X. Belaunzaran thanks the Department of Economic Development & Competitiveness of the Basque Government for his doctoral fellowship, and N. Aldai thanks the Spanish Ministry of Economy & Competitiveness and the University of the Basque Country (UPV/EHU) for her contract through the 'Ramon y Cajal (RYC-2011-08593)' program. The study was funded by the UPV/EHU (EHUA 13/29; ZALDITRANS Project) and the Department of Education, Language Policy and Culture of the Basque Government (IT766-13).Peer Reviewe

Caracterización comercial y nutricional de la grasa subcutánea de terneros y vacas de desvieje sacrificados en la Comunidad Autónoma del País Vasco

The present work was undertaken to compare the commercial carcass quality and the fatty acid composition of subcutaneous fat from Friesian culled cows of over 30 months of age, and calves of 12 months of age from Pirenaica and Salers breeds slaughtered in a commercial abattoir in the Basque Country. Friesian culled cows provided lighter carcasses (p<0.05), although similar to calves from Pirenaica breed, with lower conformation (p<0.001) and less fat cover (p<0.01) compared to other groups of calves. The subcutaneous fat of culled cows provided higher content of saturated (p<0.001) and branched-chain fatty acids (p<0,05), and lower content of monounsa-turated (p<0.01) and polyunsaturated fatty acids (p<0,001) compared to calves from Pirenaica and Salers breeds. The percentage of trans-monounsaturated fatty acids was significantly higher in the subcutaneous fat of calves from Pirenaica breed (p<0.001), while the 10 t-18:1 content was significantly higher in the fat of calves from Pirenaica breed, intermediate in the fat of calves from Salers breed, and lower in the fat of culled cows (p<0.001). These provided a 10t/11t ratio <1 and a healthier relative trans-18:1 and conjugated linoleic acid isomeric profile in the fat of Friesian culled cows compared to other groups of calves, which may provide culled cow carcasses with a higher added value from the commercialization point of view and of the profitability of cattle farms in the Basque Country.Este trabajo se ha llevado a cabo con el objetivo de comparar la calidad comercial de la canal y la composición de ácidos grasos de la grasa subcutánea de vacas de desvieje de raza Frisona de más de 30 meses de edad, y de terneros de 12 meses de edad de las razas Pirenaica y Salers sacrificados en un matadero comercial de la Comunidad Autónoma del País Vasco. Las vacas de raza Frisona presentaron canales de menor peso (p<0,05), aunque similar al grupo de los terneros de raza Pirenaica y menor conformación (p<0,001) y estado de engrasamiento (p<0,01) en comparación con los grupos de terneros. La grasa subcutánea de las vacas de desvieje presentó un mayor contenido de ácidos grasos saturados (p<0,001) y ramificados (p<0,05), y un menor contenido de ácidos grasos monoinsaturados (p<0,01) y poliinsaturados (p<0,001) en comparación con la de los terneros de las razas Pirenaica y Salers. El porcentaje de ácidos grasos monoinsaturados de tipo trans fue significativamente superior en la grasa subcutánea de los terneros de raza Pirenaica (p<0,001), mientras que el contenido de 10t-18:1 fue significativamente mayor en la grasa de terneros de raza Pirenaica, intermedio en la grasa de terneros de raza Salers, y menor en la grasa de las vacas de desvieje (p<0,001). Esto dio lugar a una relación 10t/11t <1 y un perfil isomérico relativo de trans-18:1 y ácidos linoleico conjugados más saludable en la grasa de las vacas de desvieje de raza Frisona en comparación con los grupos de terneros. Esta idea podría contribuir a la valorización de las canales procedentes de vacas de desvieje desde el punto de vista de su comercialización y de la rentabilidad de las explotaciones ganaderas de vacuno en la Comunidad Autónoma del País Vasco

Horse-meat for human consumption - Current research and future opportunities

8 páginas, 2 tablas, 3 figuras.© 2015 Elsevier Ltd. The consumption of horse-meat is currently not popular in most countries, but because of its availability and recognized nutritional value consumption is slowly increasing in several western European countries based on claims that it could be an alternative red meat. In this review, horse-meat production, trade and supply values have been summarized. In addition, the advantage of horse production is noted because of its lower methane emissions and increased uptake, particularly of n-3 polyunsaturated fatty acids (PUFAs), which is based on its digestive physiology. Of particular interest in this review is the unique fatty acid composition of horse-meat with its high level of the nutritionally desirable PUFAs in both the adipose and muscle fat. Because of its large frame size and digestive physiology, the horse can be considered an alternative to bovine meat, with large advantages regarding the maintenance of less favored mountain grazing areas and its facility to transfer PUFA from feed to meat.X. Belaunzaran thanks the Department of Economic Development & Competitiveness of the Basque Government for his predoctoral fellowship, and N. Aldai thanks the Spanish Ministry of Economy & Competitiveness and the University of the Basque Country (UPV/EHU) for her contract through the ‘Ramón y Cajal (RYC-2011-08593)’ program. The study was funded by the UPV/EHU (EHUA 13/29; ZALDITRANS Project)Peer Reviewe

HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats

OBJECTIVES HLA-B27 is a common genetic risk factor for the development of Spondyloarthritides (SpA). HLA-B27 can misfold to form cell-surface heavy chain homodimers (B272) and induce pro-inflammatory responses that may lead to SpA pathogenesis. The presence of B272 can be detected on leukocytes of HLA-B27+ Ankylosing spondylitis (AS) patients and HLA-B27 transgenic rats. We characterized a novel B272-specific monoclonal antibody to study its therapeutic use in HLA-B27 associated disorders. METHODS The monoclonal HD5 antibody was selected from a phage library to target cell-surface B272 homodimers and characterized for affinity, specificity and ligand binding. The immune modulating effect of HD5 was tested in HLA-B27 transgenic rats. Onset and progression of disease profiles were monitored during therapy. Cell-surface B272 and expansion of pro-inflammatory cells from blood, spleen and draining lymph nodes were assessed by flow cytometry. RESULTS HD5 bound B272 with high specificity and affinity (Kd = 0.32 nM). HD5 blocked cell-surface interaction of B272 with immune regulatory receptors KIR3DL2, LILRB2 and Pirb. In addition, HD5 modulated the production of TNF from CD4+ T-cells by limiting B272 interactions in vitro. In an HLA-B27 transgenic rat model repetitive dosing of HD5 reduced the expansion of pro-inflammatory CD4+ T-cells, and decreased the levels of soluble TNF and number of cell-surface B272 molecules. CONCLUSION HD5 predominantly inhibits early TNF production and expansion of pro-inflammatory CD4+ T-cells in HLA-B27 transgenic rats. Monoclonal antibodies targeting cell-surface B272 propose a new concept for the modulation of inflammatory responses in HLA-B27 related disorders