783 research outputs found
Determination of Staphylococcal Phenol-Soluble Modulins (PSMs) by a High-Resolution HPLC-QTOF System
Phenolâsoluble modulins (PSMs) are multifunctional, amphipathic, αâhelical peptides produced by virtually all staphylococcal strains. They have recently drawn much attention owing to the key contribution of some PSM peptides to staphylococcal virulence, in particular in highly virulent Staphylococcus aureus. High concentration of PSMs may cause cytolysis, damaging neutrophils, immune cells, red blood cells and white blood cells. Furthermore, all PSMs contribute to biofilm structuring and the dissemination of biofilmâassociated infection. Here we describe a method for PSM analysis in S. aureus by highâperformance liquid chromatographyâquadrupole timeâofâflight mass spectrometry system (HPLCâQTOF)
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