Elastic-plastic endochronic constitutive model of 0Crl7Ni4Cu4Nb stainless steels

We presented an elastic-plastic endochronic constitutive model of 0Crl7Ni4Cu4Nb stainless steel based on the plastic endochronic theory (which does not need the yield surface) and experimental stress-strain curves. The key feature of the model is that it can precisely describe the relation of stress and strain under various loading histories, including uniaxial tension, cyclic loading-unloading, cyclic asymmetric-stress axial tension and compression, and cyclic asymmetric-stress axial tension and compression. The effects of both mean stress and amplitude of stress on hysteresis loop based on the elastic-plastic endochronic constitutive model were investigated. Compared with the experimental and calculated results, it is demonstrated that there was a good agreement between the model and the experiments. Therefore, the elastic-plastic endochronic constitutive model provides a method for the accurate prediction of mechanical behaviors of 0Crl7Ni4Cu4Nb stainless steel subjected to various loadings

Data-Driven High-Throughput Rational Design of Double-Atom Catalysts for Oxygen Evolution and Reduction

Surging interests exist in double-atom catalysts (DACs), which not only inherit the advantages of single-atom catalysts (SACs) (e.g., ultimate atomic utilization, high activity, and selectivity) but also overcome the drawbacks of SACs (e.g., low loading and isolated active site). The design of DACs, however, remains cost-prohibitive for both experimental and computational studies, due to their huge design space. Herein, by means of density functional theory (DFT) and topological information-based machine-learning (ML) algorithms, we present a data-driven high-throughput design principle to evaluate the stability and activity of 16 767 DACs for oxygen evolution (OER) and oxygen reduction (ORR) reactions. The rational design reveals 511 types of DACs with OER activity superior to IrO2 (110), 855 types of DACs with ORR activity superior to Pt (111), and 248 bifunctional DACs with high catalytic performance for both OER and ORR. An intrinsic descriptor is revealed to correlate the catalytic activity of a DAC with the electronic structures of the DAC and its bonding carbon surface structure. This data-driven high-throughput approach not only yields remarkable prediction precision (>0.926 R-squared) but also enables a notable 144 000-fold reduction of screening time compared with pure DFT calculations, holding promise to drastically accelerate the design of high-performance DACs.https://doi.org/10.1002/adfm.20220343

Rarefaction effects in head-on collision of two identical droplets

The head-on collision of two identical droplets is investigated based on the BGK-Boltzmann equation. Gauss-Hermite quadratures with different degree of precision are used to solve the kinetic equation, so that the continuum (solution truncated at the Navier-Stokes order) and non-continuum (rarefied gas dynamics) solutions can be compared. When the kinetic equation is solved with adequate accuracy, prominent variations of the vertical velocity (the collision is in the horizontal direction), the viscous stress components, and droplet morphology are observed during the formation of liquid bridge, which demonstrates the importance of the rarefaction effects and the failure of the Navier-Stokes equation. The rarefaction effects change the topology of streamlines near the droplet surface, suppress the high-magnitude vorticity concentration inside the interdroplet region, and promote the vorticity diffusion around outer droplet surface. Two physical mechanisms responsible for the local energy conversion between the free and kinetic energies are identified, namely, the total pressure-dilatation coupling effect and the interaction between the density gradient and strain rate tensor. An energy conversion analysis is performed to show that the rarefaction effects can enhance the conversion from free energy to kinetic energy and facilitate the discharge of interdroplet gas film along the vertical direction, thereby boosting droplet coalescence

Planned sheltering as an adaptation strategy to climate change: Lessons learned from the severe flooding in Anhui Province of China in 2016

Background: Evacuation and sheltering is both a disaster response measure and a strategy to adapt to climate change, and consequently address the Sustainable Development Goals. Research has found that displacement does cause negative health impacts to evacuees, but few studies have observed how planned sheltering might reduce adverse health impacts. This article identifies the good practice and lessons learned from China's response to severe flooding in Anhui province in 2016. Methods: First, we identified the key phases for disaster sheltering by analyzing related government reports. We then interviewed 21 relevant professionals in order to identify good practice and lessons learned which could lead to better health outcomes (e.g., reduce fatalities, infectious diseases, and mental health problems). Interviewees were selected through a purposive sampling strategy, which identified emergency management professionals and those who had been assigned evacuation, sheltering, or medical tasks. Finally, thematic analysis and the constant comparative method were used to code, identify, and describe the good practice and challenges during key phases. Results: Good practice included: using early warning systems to advise communities of risks and enforce evacuation in the flood zone; preparing and using schools as shelters with open-ended periods of operation; and, providing stable shelter accommodations which offered medical and public health services, clean drinking water and food, sanitation, and toilet hygiene through multiagency cooperation. Challenges included: providing mental health services, evaluating intervention effectiveness, managing volunteers, monitoring long-term health effects, and providing economic support. Conclusions: The unintended negative effects caused by sheltering during extreme weather can be reduced. This requires close cooperation among government entities to establish planned mass shelters with appropriate levels of personal, environmental and healthcare support and to ensure long-term physical and mental health support. Additionally, if disaster mitigation strategies are integrated with climate adaptation plans, we can design more health-oriented and sustainable cities.</p

Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB/MAPK Signaling Pathway

Andrographolide, the main active component extracted from Andrographis paniculata (Burm.f.) Wall. ex Nees, exerts anti-inflammatory effects; however, the principal molecular mechanisms remain unclear. The objective of this study was to investigate the molecular mechanisms of Andrographolide in modifying lipopolysaccharide- (LPS-) induced signaling pathway in RAW264.7 cells. An in vitro model of inflammation was induced by LPS in mouse RAW264.7 cells in the presence of Andrographolide. The concentration and expression levels of proinflammatory cytokines were determined by an enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. The nuclear level of NF-κB was measured by an electrophoretic mobility shift assay (EMSA). The expression levels of NF-κB, p38, ERK, and JNK were determined by western blot. Andrographolide dose-dependently inhibited the release and mRNA expression of TNF-α, IL-6, and IL-1β in LPS-stimulated RAW264.7 cells. The nuclear level of p65 protein was decreased in Andrographolide treatment group. Western blot analysis showed that Andrographolide suppressed LPS-induced NF-κB activation and the phosphorylation of IkBa, ERK1/2, JNK, and p38. These results suggest that Andrographolide exerts an anti-inflammatory effect by inhibiting the activation of NF-κB/MAPK signaling pathway and the induction of proinflammatory cytokines

The Rice AAA-ATPase OsFIGNL1 Is Essential for Male Meiosis

Meiosis is crucial in reproduction of plants and ensuring genetic diversity. Although several genes involved in homologous recombination and DNA repair have been reported, their functions in rice (Oryza sativa) male meiosis remain poorly understood. Here, we isolated and characterized the rice OsFIGNL1 (OsFidgetin-like 1) gene, encoding a conserved AAA-ATPase, and explored its function and importance in male meiosis and pollen formation. The rice Osfignl1 mutant exhibited normal vegetative growth, but failed to produce seeds and displayed pollen abortion phenotype. Phenotypic comparisons between the wild-type and Osfignl1 mutant demonstrated that OsFIGNL1 is required for anther development, and that the recessive mutation of this gene causes male sterility in rice. Complementation and CRISPR/Cas9 experiments demonstrated that wild-type OsFIGNL1 is responsible for the male sterility phenotype. Subcellular localization showed that OsFIGNL1-green fluorescent protein was exclusively localized in the nucleus of rice protoplasts. Male meiosis in the Osfignl1 mutant exhibited abnormal chromosome behavior, including chromosome bridges and multivalent chromosomes at diakinesis, lagging chromosomes, and chromosome fragments during meiosis. Yeast two-hybrid assays demonstrated OsFIGNL1 could interact with RAD51A1, RAD51A2, DMC1A, DMC1B, and these physical interactions were further confirmed by BiFC assay. Taken together, our results suggest that OsFIGNL1 plays an important role in regulation of male meiosis and anther development

WB1, a Regulator of Endosperm Development in Rice, Is Identified by a Modified MutMap Method

Abnormally developed endosperm strongly affects rice (Oryza sativa) appearance quality and grain weight. Endosperm formation is a complex process, and although many enzymes and related regulators have been identified, many other related factors remain largely unknown. Here, we report the isolation and characterization of a recessive mutation of White Belly 1 (WB1), which regulates rice endosperm development, using a modified MutMap method in the rice mutant wb1. The wb1 mutant develops a white-belly endosperm and abnormal starch granules in the inner portion of white grains. Representative of the white-belly phenotype, grains of wb1 showed a higher grain chalkiness rate and degree and a lower 1000-grain weight (decreased by ~34%), in comparison with that of Wild Type (WT). The contents of amylose and amylopectin in wb1 significantly decreased, and its physical properties were also altered. We adopted the modified MutMap method to identify 2.52 Mb candidate regions with a high specificity, where we detected 275 SNPs in chromosome 4. Finally, we identified 19 SNPs at 12 candidate genes. Transcript levels analysis of all candidate genes showed that WB1 (Os04t0413500), encoding a cell-wall invertase, was the most probable cause of white-belly endosperm phenotype. Switching off WB1 with the CRISPR/cas9 system in Japonica cv. Nipponbare demonstrates that WB1 regulates endosperm development and that different mutations of WB1 disrupt its biological function. All of these results taken together suggest that the wb1 mutant is controlled by the mutation of WB1, and that the modified MutMap method is feasible to identify mutant genes, and could promote genetic improvement in rice