150 research outputs found
(E)-N′-[4-(DimethylÂamino)ÂbenzylÂidene]-4-methylÂbenzohydrazide methanol monosolvate
In the title compound, C17H19N3O·CH3OH, the hydrazone molÂecule exists in a trans geometry with respect to the methylÂidene unit and the dihedral angle between the two substituted benzene rings is 42.6 (2)°. In the crystal, the components are linked through N—H⋯O and O—H⋯O hydrogen bonds, forming [100] chains of alternating hydrazone and methanol molÂecules
When Distributed Consensus Meets Wireless Connected Autonomous Systems: A Review and A DAG-based Approach
The connected and autonomous systems (CAS) and auto-driving era is coming
into our life. To support CAS applications such as AI-driven decision-making
and blockchain-based smart data management platform, data and message
exchange/dissemination is a fundamental element. The distributed message
broadcast and forward protocols in CAS, such as vehicular ad hoc networks
(VANET), can suffer from significant message loss and uncertain transmission
delay, and faulty nodes might disseminate fake messages to confuse the network.
Therefore, the consensus mechanism is essential in CAS with distributed
structure to guaranteed correct nodes agree on the same parameter and reach
consistency. However, due to the wireless nature of CAS, traditional consensus
cannot be directly deployed. This article reviews several existing consensus
mechanisms, including average/maximum/minimum estimation consensus mechanisms
that apply on quantity, Byzantine fault tolerance consensus for request, state
machine replication (SMR) and blockchain, as well as their implementations in
CAS. To deploy wireless-adapted consensus, we propose a Directed Acyclic Graph
(DAG)-based message structure to build a non-equivocation data dissemination
protocol for CAS, which has resilience against message loss and unpredictable
forwarding latency. Finally, we enhance this protocol by developing a
two-dimension DAG-based strategy to achieve partial order for blockchain and
total order for the distributed service model SMR
Study on a High-Accuracy Real-Time Algorithm to Estimate SOC of Multiple Battery Cells Simultaneously
In traditional battery equalization strategy, open-circuit voltage (OCV) of battery cells was used to judge the difference of SOC between them. However, OCV is not only determined by SOC but also influenced by internal resistance, polarization voltage, capacity, and other nonlinear factors. As a result, OCV is not an ideal indicator of SOC differences, especially in transient conditions. In order to control battery consistency accurately, it is best to use SOC directly as standard for battery consistency judgment and control. To achieve this, an algorithm that can estimate SOC of multiple battery cells simultaneously with low computational complexity and high accuracy is needed. Limited by computing speed of Battery Control Unit (BCU), existing SOC estimation method is hard to estimate SOC of each battery cell simultaneously with high accuracy. In this research, a new SOC estimation strategy was proposed to estimate SOC of multiple battery cells simultaneously for battery equalization control. Battery model is established based on experimental data, and a processor-in-the-loop test system was established to verify the actual performance of the proposed algorithm. Results of simulation and test indicate that the proposed algorithm can estimate SOC of multiple battery cells simultaneously and achieved good real-time performance and high accuracy
Stability and genetic insights of the co-existence of blaCTX-M-65, blaOXA-1, and mcr-1.1 harboring conjugative IncI2 plasmid isolated from a clinical extensively-drug resistant Escherichia coli ST744 in Shanghai
BackgroundCo-existence of colistin, β-lactam and carbapenem in multidrug-resistant Enterobacteriaceae isolates poses a serious threat to public health. In this study, we investigated and characterized the co-occurrence of blaCTX-M-65, blaOXA-1, and mcr-1.1 strain isolated from a clinical extensively-drug-resistant Escherichia coli ST744 in Shanghai.MethodsAntimicrobial susceptibility test was carried out by agar dilution methods. Whole genome sequencing was conducted, and resistance genes, and sequence types of colistin in E. coli isolates were analyzed. Plasmid stability and amino acid mutations were assessed in E. coli isolates.ResultsA colistin resistant E. coli ST744, named ECPX221, was identified out of 145 fecal samples collected. The strain carries a 60,168 IncI2 plasmid with the mcr-1.1 gene. The strain also has blaCTX-M-65, blaOXA-1, dfrA14, qnrS1, cmlA5, arr2, ampC, aph(4)-Ia, sul1, and aadA5 resistance genes. The plasmid pECPX221 was capable of conjugation with an efficiency of 2.6 × 10−2. Notably, 45% of the transconjugants were determined as mcr-1.1-harboring in the colistin-free environment after 60 generation of passage. No mutations occurred in pmrB, mgrB, and phoPQ gene in the mcr-1.1-harboring transconjugants. Bioinformatic analysis indicated pECPX221 shared highly similar backbone with the previously reported mcr-1.1-harboring pAH62-1, pMFDS1339.1, pSCZE4, and p2018-10-2CC. Furthermore, sequencing and phylogenetic analyses revealed a similarity between other MCR-1-homolog proteins, indicating that ECPX221 was colistin resistant.ConclusionThe stable transferable mcr-1.1-harboring plasmid found in the E. coli ST744 strain indicated the high risk to disseminate the extensively-drug-resistance phenotype among Enterobacteriaceae
- …