Differentiation plasticity of stromal vascular fraction cells towards adipose tissue and endothelial structures

Recent research findings postulate that adipocytes and endothelial cells (ECs) may share a common progenitor. However, the interlinking pathways between adipose tissue and endo-thelium, and the differentiation potential of cells to convert from one tissue into the other via progenitor cells, have not been elucidated so far and are therefore the focus of this thesis. Stromal vascular fraction (SVF) cells were isolated from liposuction aspirates or excised adi-pose tissue and separated into CD31+ and CD31- populations by magnet-assisted cell sorting. Differentiation to fat tissue was induced in both CD31 fractions. Differentiation was assayed enzymatically and by cell counting. Maturation to endothelium was performed with vascular endothelial growth factor, insulin like growth factor-1 plus 2% FCS and confirmed by flow cytometry and tube formation assays on Matrigel™. The results presented here show that the SVF contains a CD31-, S100+ cell type which can differentiate into adipocytes and EC. The SVF also comprises CD31+ cells that, although they have an endothelial phenotype, can be converted into mature adipocytes. These findings demonstrate the potency of SVF cells to perform both adipogenic and endothelial differentiation. Further, they reveal the plasticity of mature cells of mesenchymal origin to undergo conversion from endothelium to adipose tis-sue and vice versa

der Medizin

plasticity of stromal vascular fraction cells towards adipose tissu