1,065 research outputs found
Splash control of drop impacts with geometric targets
Drop impacts on solid and liquid surfaces exhibit complex dynamics due to the
competition of inertial, viscous, and capillary forces. After impact, a liquid
lamella develops and expands radially, and under certain conditions, the outer
rim breaks up into an irregular arrangement of filaments and secondary
droplets. We show experimentally that the lamella expansion and subsequent
break up of the outer rim can be controlled by length scales that are of
comparable dimension to the impacting drop diameter. Under identical impact
parameters, ie. fluid properties and impact velocity, we observe unique
splashing dynamics by varying the target cross-sectional geometry. These
behaviors include: (i) geometrically-shaped lamellae and (ii) a transition in
splashing stability, from regular to irregular splashing. We propose that
regular splashes are controlled by the azimuthal perturbations imposed by the
target cross-sectional geometry and that irregular splashes are governed by the
fastest-growing unstable Plateau-Rayleigh mode
Proteolytic cleavage of pertussis toxin S1 subunit is not essential for its activity in mammalian cells
BACKGROUND: Pertussis toxin (PT) is an exotoxin virulence factor produced by Bordetella pertussis, the causative agent of whooping cough. PT consists of an active subunit (S1) that ADP-ribosylates the alpha subunit of several mammalian G proteins, and a B oligomer (S2–S5) that binds glycoconjugate receptors on cells. PT appears to enter cells by endocytosis, and retrograde transport through the Golgi apparatus may be important for its cytotoxicity. A previous study demonstrated that proteolytic processing of S1 occurs after PT enters mammalian cells. We sought to determine whether this proteolytic processing of S1 is necessary for PT cytotoxicity. RESULTS: Protease inhibitor studies suggested that S1 processing may involve a metalloprotease, and processing does not involve furin, a mammalian cell protease that cleaves several other bacterial toxins. However, inhibitor studies showed a general lack of correlation of S1 processing with PT cellular activity. A combination of replacement, insertion and deletion mutations in the C-terminal region of S1, as well as mass spectrometry data, suggested that the cleavage site is located around residue 203–204, but that cleavage is not strongly sequence-dependent. Processing of S1 was abolished by each of 3 overlapping 8 residue deletions just downstream of the putative cleavage site, but not by smaller deletions in the same region. Processing of the various mutant forms of PT did not correlate with cellular activity of the toxin, nor with the ability of the bacteria producing them to infect the mouse respiratory tract. In addition, S1 processing was not detected in transfected cells expressing S1, even though S1 was fully active in these cells. CONCLUSIONS: S1 processing is not essential for the cellular activity of PT. This distinguishes it from the processing of various other bacterial toxins, which has been shown to be important for their cytotoxicity. S1 processing may be mediated primarily by a metalloprotease, but the cleavage site on S1 is not sequence-dependent and processing appears to depend on the general topology of the protein in that region, indicating that multiple proteases may contribute to this cleavage
Drop Splashing on a Dry Smooth Surface
The corona splash due to the impact of a liquid drop on a smooth dry
substrate is investigated with high speed photography. A striking phenomenon is
observed: splashing can be completely suppressed by decreasing the pressure of
the surrounding gas. The threshold pressure where a splash first occurs is
measured as a function of the impact velocity and found to scale with the
molecular weight of the gas and the viscosity of the liquid. Both experimental
scaling relations support a model in which compressible effects in the gas are
responsible for splashing in liquid solid impacts.Comment: 11 pages, 4 figure
Making a splash with water repellency
A 'splash' is usually heard when a solid body enters water at large velocity.
This phenomena originates from the formation of an air cavity resulting from
the complex transient dynamics of the free interface during the impact. The
classical picture of impacts on free surfaces relies solely on fluid inertia,
arguing that surface properties and viscous effects are negligible at
sufficiently large velocities. In strong contrast to this large-scale
hydrodynamic viewpoint, we demonstrate in this study that the wettability of
the impacting body is a key factor in determining the degree of splashing. This
unexpected result is illustrated in Fig.1: a large cavity is evident for an
impacting hydrophobic sphere (1.b), contrasting with the hydrophilic sphere's
impact under the very same conditions (1.a). This unforeseen fact is
furthermore embodied in the dependence of the threshold velocity for air
entrainment on the contact angle of the impacting body, as well as on the ratio
between the surface tension and fluid viscosity, thereby defining a critical
capillary velocity. As a paradigm, we show that superhydrophobic impacters make
a big 'splash' for any impact velocity. This novel understanding provides a new
perspective for impacts on free surfaces, and reveals that modifications of the
detailed nature of the surface -- involving physico-chemical aspects at the
nanometric scales -- provide an efficient and versatile strategy for
controlling the water entry of solid bodies at high velocity.Comment: accepted for publication in Nature Physic
Phase Transitions in a Model Anisotropic High Tc Superconductor
We carry out simulations of the anisotropic uniformly frustrated 3D XY model,
as a model for vortex line fluctuations in high Tc superconductors. We compute
the phase diagram as a function of temperature and anisotropy, for a fixed
applied magnetic field. We find that superconducting coherence parallel to the
field persists into the vortex line liquid state, and that this transition lies
well below the "mean-field" cross-over from the vortex line liquid to the
normal state.Comment: 23 pages + 19 ps figure
Kinetic Theory of Flux Line Hydrodynamics:LIQUID Phase with Disorder
We study the Langevin dynamics of flux lines of high--T superconductors
in the presence of random quenched pinning. The hydrodynamic theory for the
densities is derived by starting with the microscopic model for the flux-line
liquid. The dynamic functional is expressed as an expansion in the dynamic
order parameter and the corresponding response field. We treat the model within
the Gaussian approximation and calculate the dynamic structure function in the
presence of pinning disorder. The disorder leads to an additive static peak
proportional to the disorder strength. On length scales larger than the line
static transverse wandering length and at long times, we recover the
hydrodynamic results of simple frictional diffusion, with interactions
additively renormalizing the relaxational rate. On shorter length and time
scales line internal degrees of freedom significantly modify the dynamics by
generating wavevector-dependent corrections to the density relaxation rate.Comment: 61 pages and 6 figures available upon request, plain TEX using
Harvard macro
Vortex Line Fluctuations in Model High Temperature Superconductors
We carry out Monte Carlo simulations of the uniformly frustrated 3d XY model
as a model for vortex line fluctuations in a high Tc superconductor. A density
of vortex lines of f=1/25 is considered. We find two sharp phase transitions.
The low T phase is an ordered vortex line lattice. The high T normal phase is a
vortex line liquid with much entangling, cutting, and loop excitations. An
intermediate phase is found which is characterized as a vortex line liquid of
disentangled lines. In this phase, the system displays superconducting
properties in the direction parallel to the magnetic field, but normal behavior
in planes perpendicular to the magnetic field.Comment: 38 pages, LaTeX 15 figures (upon request to
[email protected]
Vortex Matter Transition in BiSrCaCuO under Tilted Fields
Vortex phase diagram under tilted fields from the axis in
BiSrCaCuO is studied by local magnetization
hysteresis measurements using Hall probes. When the field is applied at large
angles from the axis, an anomaly () other than the well-known
peak effect () are found at fields below . The angular dependence of
the field is nonmonotonic and clearly different from that of
and depends on the oxygen content of the crystal. The results suggest existence
of a vortex matter transition under tilted fields. Possible mechanisms of the
transition are discussed.Comment: Revtex, 4 pages, some corrections are adde
Can agricultural cultivation methods influence the healthfulness of crops for foods
The aim of the current study was to investigate if there are any health effects of long-term consumption of organically grown crops using a rat model. Crops were retrieved over two years from along-term field trial at three different locations in Denmark, using three different cultivation systems(OA, organic based on livestock manure; OB, organic based on green manure; and C, conventional with mineral fertilizers and pesticides)with two field replicates. The cultivation system had an impact on the nutritional quality, affecting γ-tocopherol, some amino acids, and fatty acid composition. Additionally, the nutritional quality was affected by harvest year and location. However, harvest year and location rather than cultivation system affected the measured health biomarkers. In conclusion, the differences in dietary treatments composed of ingredients from different cultivation systems did not lead to significant differences in the measured health biomarkers, except for a significant difference in plasma IgGl evels
Paleophysical Oceanography with an Emphasis on Transport Rates
Paleophysical oceanography is the study of the behavior of the fluid ocean of the past, with a specific emphasis on its climate implications, leading to a focus on the general circulation. Even if the circulation is not of primary concern, heavy reliance on deep-sea cores for past climate information means that knowledge of the oceanic state when the sediments were laid down is a necessity. Like the modern problem, paleoceanography depends heavily on observations, and central difficulties lie with the very limited data types and coverage that are, and perhaps ever will be, available. An approximate separation can be made into static descriptors of the circulation (e.g., its water-mass properties and volumes) and the more difficult problem of determining transport rates of mass and other properties. Determination of the circulation of the Last Glacial Maximum is used to outline some of the main challenges to progress. Apart from sampling issues, major difficulties lie with physical interpretation of the proxies, transferring core depths to an accurate timescale (the “age-model problem”), and understanding the accuracy of time-stepping oceanic or coupled-climate models when run unconstrained by observations. Despite the existence of many plausible explanatory scenarios, few features of the paleocirculation in any period are yet known with certainty.National Science Foundation (U.S.) (grant OCE-0645936
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