Predictive genetic plan for a captive population of the Chinese goral (Naemorhedus griseus) and prescriptive action for ex situ and in situ conservation management in Thailand

Captive breeding programs for endangered species can increase population numbers for eventual reintroduction to the wild. Captive populations are typically small and isolated, which results in inbreeding and reduction of genetic variability, and may lead to an increased risk of extinction. The Omkoi Wildlife Breeding Center maintains the only Thai captive Chinese goral (Naemorhedus griseus) population, and has plans to reintroduce individuals into natural isolated populations. Genetic variability was assessed within the captive population using microsatellite data. Although no bottleneck was observed, genetic variability was low (allelic richness = 7.091 ± 0.756, He = 0.455 ± 0.219; He < Ho) and 11 microsatellite loci were informative that likely reflect inbreeding. Estimates of small effective population size and limited numbers of founders, combined with wild-born individuals within subpopulations, tend to cause reduction of genetic variability over time in captive programs. This leads to low reproductive fitness and limited ability to adapt to environmental change, thereby increasing the risk of extinction. Management of captive populations as evolutionarily significant units with diverse genetic backgrounds offers an effective strategy for population recovery. Relocation of individuals among subpopulations, or introduction of newly captured wild individuals into the captive program will help to ensure the future security of Chinese goral. Implications for future conservation actions for the species are discussed herein.Funding provided by: National Research Council of ThailandCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100004704Award Number: 404639Funding provided by: Undergraduate Research Matching FundCrossref Funder Registry ID: Award Number: 6501.0901/0775Funding provided by: Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart UniversityCrossref Funder Registry ID: Award Number: CASTNAR, NRU-KU, ThailandFunding provided by: Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Office of Higher Education Commission, Ministry of EducationCrossref Funder Registry ID: Award Number: AG-BIO/PERDO-CH

Introduction of wild Chinese gorals into a captive population requires careful genetic breeding plan monitoring for successful long-term conservation

The Chinese goral (Naemorhedus griseus) is a small goat-like animal, which is considered “vulnerable” due to its rapid decline in population in the wild. Captive breeding programs are necessary to prevent the extinction of Chinese gorals; however, reproduction in captivity reduces genetic diversity due to inbreeding. In 2020, a total of six wild Chinese gorals were introduced into a captive population of 73 individuals to improve the allelic gene pool. An assessment of captive gorals was conducted to trace and understand genetic diversity in the new captive state. Microsatellite genotyping and mitochondrial D-loop sequence analyses were performed to examine the genetic diversity and population structure. The results showed very low haplotype diversity, with a significant difference between He (0.477 ± 0.065) and Ho (0.196 ± 0.056), suggesting a high degree of inbreeding. This resulted in a limited ability to adapt to environmental change and low natural reproductive fitness, thus increasing the risk of population decline and eventual extinction. Management of captive breeding plans based on different subpopulations and haplotypes has been proposed to maximize genetic variability and enhance the success of future conservation plans