2,037 research outputs found
Preparative Synthesis of dTDP-L-Rhamnose Through Combined Enzymatic Pathways
dTDP-L-rhamnose, an important precursor of O-antigen, was prepared on a large scale from dTMP by executing an one-pot reaction in which six enzymes are involved. Two enzymes, dTDP-4-keto-6-deoxy-D-glucose 3,5-epimerase and dTDP-4-keto-rhamnose reductase, responsible for the conversion of dTDP-4-keto-6-deoxy- D-glucose to dTDP-L-rhamnose, were isolated from their putative sequences in the genome of Mesorhizobium loti, functionally expressed in Escherichia coli, and their enzymatic activities were identified. The two enzymes were combined with an enzymatic process for dTDP-4- keto-6-deoxy-D-glucose involving TMP kinase, acetate kinase, dTDP-glucose synthase, and dTDP-glucose 4,6- dehydratase, which allowed us to achieve a preparative scale synthesis of dTDP-L-rhamnose using dTMP and glucose-1-phosphate as starting materials. About 82% yield of dTDP-L-rhamnose was obtained based on initial dTMP concentration at 20 mM dTMP, 1 mM ATP, 10 mM NADH, 60 mM acetyl phosphate, and 80 mM glucose-1- phosphate. From the reaction with 20 ml volume, approximately 180 mg of dTDP-L-rhamnose was obtained in an overall yield of 60% after two-step purification, that is, anion exchange chromatography and gel filtration for desalting. The purified product was identifiedbyHPLC, ESI-MS,andNMR,showingabout95%purity
Selective inhibition of liver X receptor α-mediated lipogenesis in primary hepatocytes by licochalcone A
Impacts of whey protein on starch digestion in rumen and small intestine of steers
Four Korean native steers (511 ± 17.2 kg; 2 × 2 replicated
crossover design) fitted with duodenal cannulas were used to investigate the
influence of oral administration of soluble whey protein (WP; 82.29%
crude protein) on ruminal fermentation, gastrointestinal (GI) hormone secretion
in the blood, pancreatic α-amylase activity in the duodenum, and
disappearance rate in each segment of the GI tract. Steers were orally fed the
basal diet (control; TMR [total mixed ration] 9 kg/d) or the basal diet with
enriched WP (400 g/d) for 14 days. The apparent crude protein disappearance rate
in the rumen of the WP was higher than in control (p <
0.05). However, no difference between groups was observed in the apparent crude
protein disappearance rate in the intestine and the apparent starch
disappearance rates in the rumen, GI tract. The level of cholecystokinin,
secretin, and ghrelin in serum and pancreatic α-amylase activity in the
duodenum of the WP also did not change. The changes in the level of blood urea
nitrogen related to protein metabolism were higher in the WP than in the control
(p < 0.05). However, the levels of total protein,
lipid, carbohydrate and mineral metabolites did not change. Consequently, we
suggest that the oral administration of WP in steers assisted in ruminal
fermentation due to the population increase of microbes in the rumen but did not
improve the starch digestion rate in the small intestine because GI hormone
secretion in the blood and pancreatic α-amylase activity did not
change
Echovirus 30 Induced Neuronal Cell Death through TRIO-RhoA Signaling Activation
BACKGROUND: Echovirus 30 (Echo30) is one of the most frequently identified human enteroviruses (EVs) causing aseptic meningitis and encephalitis. However the mechanism underlying the pathogenesis of Echo30 infection with significant clinical outcomes is not completely understood. The aim of this investigation is to illustrate molecular pathologic alteration in neuronal cells induced by Echo30 infection using clinical isolate from young patient with neurologic involvement. METHODOLOGY/PRINCIPAL FINDINGS: To characterize the neuronal cellular response to Echo30 infection, we performed a proteomic analysis based on two-dimensional gel electrophoresis (2-DE) and MALDI-TOF/TOF Mass Spectrophotometric (MS) analysis. We identified significant alteration of several protein expression levels in Echo30-infected SK-N-SH cells. Among these proteins, we focused on an outstanding up-regulation of Triple functional domain (TRIO) in Echo30-infected SK-N-SH cells. Generally, TRIO acts as a key component in the regulation of axon guidance and cell migration. In this study, we determined that TRIO plays a role in the novel pathways in Echo30 induced neuronal cell death. CONCLUSIONS/SIGNIFICANCE: Our finding shows that TRIO plays a critical role in neuronal cell death by Echo30 infection. Echo30 infection activates TRIO-guanine nucleotide exchange factor (GEF) domains (GEFD2) and RhoA signaling in turn. These results suggest that Echo30 infection induced neuronal cell death by activation of the TRIO-RhoA signaling. We expect the regulation of TRIO-RhoA signaling may represent a new therapeutic approach in treating aseptic meningitis and encephalitis induced by Echo30
Tau positron emission tomography in tauopathies: A narrative review
Aggregation of misfolded tau in the brain is a major pathological feature common in various neurodegenerative disorders known as tauopathies, including Alzheimer’s disease, progressive supranuclear palsy, corticobasal syndrome, and dementia with Lewy bodies. Tauopathies are collection of diseases with varied overlapping symptoms and complicated manifestations. Consequently, it is crucial to be able to assess tau deposits in vivo. Over the past decade, tau-specific radioligands for positron emission tomography (PET) have been developed and tested, including first-generation compounds (e.g., 18F-THK5317, 18F-THK5351, 18F-AV1451, and 11C-PBB3) and second-generation compounds (18F-MK-6240, 18F-RO-948, and 18F-PI-2620). With the recent advances of tau PET tracers, assessing the pattern of tau deposition in vivo is possible. These methods will allow accurate diagnosis of tauopathies and monitoring of disease progression. In this mini review, we summarize current findings from studies using tau PET tracers regarding neuropathological characteristics, clinical implications, and potential applications of tau PET. We also discuss methodological considerations for appropriate use of these technologies and discuss what has been learned from these findings
De novo formation of basal bodies in Naegleria gruberi: regulation by phosphorylation
The de novo formation of basal bodies in Naegleria gruberi was preceded by the transient formation of a microtubule (MT)-nucleating complex containing γ-tubulin, pericentrin, and myosin II complex (GPM complex). The MT-nucleating activity of GPM complexes was maximal just before the formation of visible basal bodies and then rapidly decreased. The regulation of MT-nucleating activity of GPM complexes was accomplished by a transient phosphorylation of the complex. Inhibition of dephosphorylation after the formation of basal bodies resulted in the formation of multiple flagella. 2D-gel electrophoresis and Western blotting showed a parallel relationship between the MT-nucleating activity of GPM complexes and the presence of hyperphosphorylated γ-tubulin in the complexes. These data suggest that the nucleation of MTs by GPM complexes precedes the de novo formation of basal bodies and that the regulation of MT-nucleating activity of GPM complexes is essential to the regulation of basal body number
TCT-664 Seven-year Clinical Outcomes of Sirolimus-Eluting Stent Versus Bare-Metal Stent; A Matched Analysis From A Real World, Single Center Registry
Toward the Evidence of the Accretion Disk Emission in the Symbiotic Star RR Tel
In this paper, we argue that in the symbiotic star RR Tel the existence of an
accretion disk around the hot companion is strongly implied by the
characteristic features exhibited by the Raman-scattered O VI lines around 6830
\AA and 7088 \AA. High degrees of polarization and double-peaked profiles in
the Raman-scattered lines and single-peak profiles for other emission lines are
interpreted as line-of-sight effects, where the H I scatterers near the giant
see an incident double-peaked profile and an observer with a low inclination
sees single-peak profiles. It is predicted that different mass concentrations
around the accretion disk formed by a dusty wind may lead to the disparate
ratios of the blue peak strength to the red counterpart observed in the 6830
and 7088 features. We discuss the evolutionary links between symbiotic stars
and bipolar protoplanetary nebulae and conclude that the Raman scattering
processes may play an important role in investigation of the physical
properties of these objects.Comment: 11 pages, 3 figures, accepted for publication in the ApJ Letter
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