Asset management for sustainable road funding

This paper lays out a framework for managing complex asset systems, such as road networks, with a view to optimising life-cycle value of the asset base. It is based on discussions at the International transport Forum Roundtable on Sustainable Road Funding held in Paris 25-26 October 20121 and includes inputs from the ITF working Group on Infrastructure Adaptation to Extreme Weather and Climate Change

Into the unknown: expression profiling without genome sequence information in CHO by next generation sequencing

The arrival of next-generation sequencing (NGS) technologies has led to novel opportunities for expression profiling and genome analysis by utilizing vast amounts of short read sequence data. Here, we demonstrate that expression profiling in organisms lacking any genome or transcriptome sequence information is feasible by combining Illumina’s mRNA-seq technology with a novel bioinformatics pipeline that integrates assembled and annotated Chinese hamster ovary (CHO) sequences with information derived from related organisms. We applied this pipeline to the analysis of CHO cells which were chosen as a model system owing to its relevance in the production of therapeutic proteins. Specifically, we analysed CHO cells undergoing butyrate treatment which is known to affect cell cycle regulation and to increase the specific productivity of recombinant proteins. By this means, we identified sequences for >13 000 CHO genes which added sequence information of ∼5000 novel genes to the CHO model. More than 6000 transcript sequences are predicted to be complete, as they covered >95% of the corresponding mouse orthologs. Detailed analysis of selected biological functions such as DNA replication and cell cycle control, demonstrated the potential of NGS expression profiling in organisms without extended genome sequence to improve both data quantity and quality

Metabolic Phenotyping of CHO Cells Varying in Cellular Biomass Accumulation and Maintenance during Fed-Batch Culture

CHO cell lines capable of high-level recombinant protein product biosynthesis during fed-batch culture are still generally obtained by intensive empirical screening of transfected cells rather than knowledge-guided cellular engineering. In this study, we investigate how CHO cell lines create and maintain cellular biosynthetic capacity during fed-batch culture to achieve the optimal combination of rapid exponential proliferation and extended maintenance of high cell biomass concentration. We perform a comparative meta-analysis of mitochondrial and glycolytic functions of 22 discrete parental CHO cell lineages varying in fed-batch culture performance to test the hypotheses that (i) "biomass-intensive" CHO cells exhibit conserved differences in metabolic programming and (ii) it is possible to isolate parental CHO cell lines with a biomass-intensive phenotype to support fed-batch bioproduction processes. We show that for most parental CHO cell lines, rapid proliferation and high late-stage culture performance are mutually exclusive objectives. However, quantitative dissection of mitochondrial and glycolytic functions revealed that a small proportion of clones utilize a conserved metabolic program that significantly enhances cellular glycolytic and mitochondrial oxidative capacity at the onset of late-stage culture. We reveal the central importance of dynamic metabolic re-programming to activate oxidative mitochondrial function as a necessary mechanism to support CHO cell biosynthetic performance during culture

PSY1330-01.General Psychology.Sp15.Wlaschin,Jhon

Goals: To introduce the content and methods of the major areas of the science of psychology. To provide a foundation for the further study of psychology. Content: Physiological processes, perception, learning and memory, cognition, emotion, development and personality, social processes, psychopathology and psychotherapy. Taught: Every semester Credits: 4 credit

PSY1330-01.General Psychology.Sp16.Wlaschin,Jhon

Goals: To introduce the content and methods of the science of psychology. To provide a foundation for the further study of psychology. Content: Physiological processes, perception, learning and memory, cognition, emotion, development and personality, social processes, psychopathology and psychotherapy. Taught: Every semester Credits:

PSY3980-02.Tpcs: Relationships and Health.Su14.Wlaschin,Jhon

This course explores how and why relationships contribute to mental and physical health. Combining psychological and public health perspectives, we will review and discuss current research, journalistic coverage, and other forms of media to examine topics such as the role of stress in health and relationships, the impact of social support on our health and well-being, and recent findings about the contagion of health factors within social networks. Prerequisite: General Psychology or permission of instructor

PSY1330-01.General Psychology.F13.Wlaschin,Jhon

Goals: To introduce the content and methods of the major areas of the science of psychology. To provide a foundation for the further study of psychology. Content: Physiological processes, perception, learning and memory, cognition, emotion, development and personality, social processes, psychopathology and psychotherapy. Taught: Every semeste