20 research outputs found
Epilithic community development on artificial reefs deployed along a cross-shelf environmental gradient off Paraná state, southern Brazil
First record of the fireworm Hermodice carunculata (Annelida, Polychaeta) preying on colonies of the fire coral Millepora alcicornis (Cnidaria, Hydrozoa)
Co-occurring morphologically distinct algae support a diverse associated fauna in the intertidal zone of Araçá Bay, Brazil
Crustáceos decápodos associados às cordas de cultivo do mexilhão Perna perna (Linnaeus, 1758) (Mollusca, Bivalvia, Mytilidae) na Enseada da Armação do Itapocoroy, Penha - SC
Documenting hurricane impacts on coral reefs using two-dimensional video-mosaic technology
The Outer Dynein Arm-Docking Complex: Composition and Characterization of a Subunit (Oda1) Necessary for Outer Arm Assembly
To learn more about how dyneins are targeted to specific sites in the flagellum, we have investigated a factor necessary for binding of outer arm dynein to the axonemal microtubules of Chlamydomonas. This factor, termed the outer dynein arm-docking complex (ODA-DC), previously was shown to be missing from axonemes of the outer dynein armless mutants oda1 and oda3. We have now partially purified the ODA-DC, determined that it contains equimolar amounts of M(r) ∼105,000 and ∼70,000 proteins plus a third protein of M(r) ∼25,000, and found that it is associated with the isolated outer arm in a 1:1 molar ratio. We have cloned a full-length cDNA encoding the M(r) ∼70,000 protein; the sequence predicts a 62.5-kDa protein with potential homologs in higher ciliated organisms, including humans. Sequencing of corresponding cDNA from strain oda1 revealed it has a mutation resulting in a stop codon just downstream of the initiator ATG; thus, it is unable to make the full-length M(r) ∼70,000 protein. These results demonstrate that the ODA1 gene encodes the M(r) ∼70,000 protein, and that the protein is essential for assembly of the ODA-DC and the outer dynein arm onto the doublet microtubule