A survey of the levels of pesticides in bees, their colonies and forage

Bees provide important and economically valuable pollination services to agriculture and other ecosystems. Recent global honeybee declines have been attributed to pesticides, which can ultimately affect the overall functioning and survival of a colony. Various routes of exposure include contaminated beeswax, pollen and nectar. This thesis presents work which examines the presence, accumulation and levels of pesticides found within a number of honeybee related matrices and bumblebee bodies, with four main aims to this study. Firstly, determine which pesticides are contained within beeswax from around the UK. Secondly, monitor which pesticides accumulate in beeswax over a two year period. Thirdly, measure residual neonicotinoid levels in oilseed rape (OSR) nectar and pollen samples. Finally, quantify the levels of thiamethoxam and metabolite clothianidin in bumblebees, following feeding trial exposure, as part of a collaborative study. Analysis was conducted using gas chromatography–mass spectrometry (GC-MS) and quadrupole time-of-flight liquid chromatography-mass spectrometry (Q-TOF LC/MS), utilising the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) extraction method. Bumblebee samples were extracted using a refined in-house procedure. Results evidenced apiculturally applied chemical treatments to be most likely found in beeswax samples whilst tau-Fluvalinate was most persistent and likely to accumulate in the hive, including foundation wax not exposed to such treatment. Varied levels of neonicotinoids were identified in hand-collected OSR nectar samples; and there require a greater level of analysis, to further understand the implications of these results on UK bees. No quantifiable levels were detected in pollen. Bumblebee analysis determined possible levels of exposure to thiamethoxam during feeding. This thesis provides the first known attempt of identifying pesticide presence and accumulation within UK beeswax, in addition to the levels within UK OSR nectar and pollen. The findings may have wider implications on the beekeeping community. Also presented are various methodologies suitable for future research

Long-term Geophysical Monitoring of Simulated Clandestine Graves using Electrical and Ground Penetrating Radar Methods: 4–6 Years After Burial

This ongoing monitoring study provides forensic search teams with systematic geophysical data over simulated clandestine graves for comparison to active cases. Simulated “wrapped,”“naked,” and “control” burials were created. Multiple geophysical surveys were collected over 6 years, here showing data from 4 to 6 years after burial. Electrical resistivity (twin electrode and ERI), multifrequency GPR, grave and background soil water were collected. Resistivity surveys revealed that the naked burial had low-resistivity anomalies up to year four but then difficult to image, whereas the wrapped burial had consistent large high-resistivity anomalies. GPR 110- to 900-MHz frequency surveys showed that the wrapped burial could be detected throughout, but the naked burial was either not detectable or poorly resolved. 225-MHz frequency GPR data were optimal. Soil water analyses showed decreasing (years 4 to 5) to background (year 6) conductivity values. Results suggest both resistivity and GPR surveying if burial style unknown, with winter to spring surveys optimal and increasingly important as time increases

A multidisciplinary scientific investigation of the 1916 Hawthorn Mine Crater, Beaumont Hamel, Somme, Northern France

Hawthorn Crater is a prominent feature of the former Somme battle-field near Beaumont Hamel, Northern France. It resulted from the detonation of arguably the most famous of nine mines that the British had prepared below German lines on 1 July 1916, as part of the opening day of the Battle of the Somme. However, the crater has not been studied scientifically, as was in private land until recently taken over by the Hawthorn Crater Association. This paper documents three field seasons of multi-disciplinary site investigations. Methods included: remote sensing, drones, ground-based-LiDAR and surface surveys, geophysics and archaeological investigations. Magnetic anomalies were identified as: still-intact German fire pits, barbed wire and equipment, as the crater became the frontline after formation, and Allied shell craters. This study provided a rare opportunity to study a First World War mine crater, and highlighting modern science can assist detection and characterisation of significant archaeological sites

Geophysical monitoring of simulated homicide burials for forensic investigations

Finding hidden bodies, believed to have been murdered and buried, is problematic, expensive in terms of human resource and currently has low success rates for law enforcement agencies. Here we present, for the first time, ten years of multidisciplinary geophysical monitoring of simulated clandestine graves using animal analogues. Results will provide forensic search teams with crucial information on optimal detection techniques, equipment configuration and datasets for comparison to active and unsolved cold case searches. Electrical Resistivity (ER) surveys showed a naked burial produced large, low-resistivity anomalies for up to four years, but then the body became difficult to image. A wrapped burial had consistent small, high-resistivity anomalies for four years, then large high-resistivity anomalies until the survey period end. Ground Penetrating Radar (GPR) 110-900 MHz surveys showed the wrapped burial could be detected throughout. 225 MHz GPR data was optimal, but the naked burial was poorly imaged after six years. Results suggested conducting both ER and GPR surveys if the burial style was unknown when searching for interred remains. Surveys in winter and spring produced the best datasets, and, as post-burial time increases, surveying in these seasons became increasingly important. This multidisciplinary study provides critical new insights for law enforcement and families of the disappeared worldwide

Monitoring of simulated clandestine graves of dismembered victims using UAVs, electrical tomography, and GPR over one year to aid investigations of human rights violations in Colombia, South America

In most Latin American countries there are significant numbers of missing people and forced disappearances, over 90,000 in Colombia alone. Successful detection of shallow buried human remains by forensic search teams is difficult in varying terrain and climates. Previous research has created controlled simulated clandestine graves of murder victims to optimize search techniques and methodologies. This paper reports on a study on controlled test site results over four simulated dismembered victims' clandestine graves as this is sadly a common scenario encountered in Latin America. Multispectral images were collected once post-burial, electrical resistivity surveys were collected 4 times and ground penetrating radar (GPR) surveys collected three times up to the end of the 371 day survey monitoring period. After data processing, results showed that the multispectral dataset could detect the simulated clandestine and control graves, with electrical resistivity imaging relative high resistances over some of the simulated graves but not over the empty control graves. GPR results showed good imaging on the Day 8 surveys, medium imaging on the Day 294 surveys and medium to good imaging on the Day 371 surveys. Study implications suggest that, whilst clandestine graves of dismembered homicide victims would likely result in smaller-sized graves when compared to graves containing intact bodies, these graves can still potentially be detected using remote sensing and geophysical methods

Wildlife crime: The application of forensic geoscience to assist with criminal investigations.

Wildlife crime is a growing problem in many rural areas. However, it can often be difficult to determine exactly what had happened and provide evidential Court material, especially where evidence is ephemeral. This paper presents a case study where a badger sett had been illegally filled and evidence was rapidly required to support a prosecution before it was either destroyed by the suspect/further badger activities or eroded by weather/time. A topographic surface survey was undertaken, quantifying the number and spatial position of sett entrances, as well as which had been filled by a slurry material. A ground penetrating radar survey was also undertaken to quantify how much tunnels were filled. Study results evidenced five sett tunnels were filled out of twelve observed. The slurry fill material was not being observed elsewhere on the surface. GPR survey data evidenced ∼1m-5m of slurry fill in tunnels. A subsequent report was forwarded to the CPS as evidential material. Study implications suggest the importance of rapid geoscience surveys to assist Police Forces to both gain scientific evidence for prosecutions and to deter future wildlife crime. [Abstract copyright: Copyright © 2018 Elsevier B.V. All rights reserved.

Detection and characterisation of Black Death burials by multi-proxy geophysical methods

The construction of the new Crossrail railway discovered 25 well preserved skeletons shallowly buried in Central London in 2013. Subsequent carbon dating and aDNA analysis confirmed the archaeological age and presence of the Yersinia pestis “Black Death” plague epidemic strain. Here we present the non-invasive multi-proxy geophysical survey of the adjacent Charterhouse Square, rapidly undertaken to detect any further burials and characterise the site. Historical records suggested the area was a burial ground for Black Death plague victims, before subsequent cemetery and urban land use. Following initial trial surveys, surveys imaged ∼200 isolated and similar-sized burials in the south-west of the site. There were also two contrasting burial orientations present at various depths which suggested a series of controlled phased burials. A well-defined eastern burial boundary, taking the form of a ditch and bank, was also discovered. Geophysical surveys also identified a subsequent complex site history with multiple-aged features. This study revises knowledge of Black Death aged-burials and provides important implications for successful geophysical burial detection with significant time- and space-limited site constraints