In vitro gene delivery with non-cytotoxic cationic oligopeptide amphiphiles

AIChE Annual Meeting, Conference Proceedings

Co-delivery of p53 and paclitaxel with cationic oligopeptide amphiphilic for cancer therapy

AIChE Annual Meeting, Conference Proceedings

Amphiphilic cationic polypeptide self-assemblies for co-delivery of anticancer drug and gene

8th World Biomaterials Congress 20082792

Self-assembled oligopeptide nanostructures for co-delivery of drug and gene with synergistic therapeutic effect

10.1016/j.biomaterials.2009.03.006Biomaterials30173100-3109BIMA

Bio-functional micelles self-assembled from a folate-conjugated block copolymer for targeted intracellular delivery of anticancer drugs

10.1016/j.biomaterials.2006.11.013Biomaterials2871423-1433BIMA

Branched disulfide-based polyamidoamines capable of mediating high gene transfection

10.2174/138161210791920504Current Pharmaceutical Design16212341-2349CPDE

Diaminododecane-based cationic bolaamphiphile as a non-viral gene delivery carrier

The advancement in gene therapy relies upon the discovery of safe and efficient delivery agents and methods In this study, we report the design and synthesis of a cationic bolaamphiphile as a non-viral gene delivery agent The bolaamphiphile is composed of 1,12-diaminododecane as the central hydrophobic unit linked to the hydrophilic pentaethylenehexamine via thioether-based glycidyl units This bolaamphiphile condensed DNA efficiently into nanoparticles of sizes around 150-200 nm with positive zeta potential of 30-35 mV In vitro luciferase expression levels and percentage of GFP expressing cells induced by the bolaamphiphile/DNA complexes were higher than those mediated by the often used " golden" standard of non-viral systems, polyethyleneimine (PEI, branched, 25 kDa) at its optimal N/P ratio in HEK293, HepG2, NIH3T3, HeLa and 4T1 cells In vitro cytotoxicity testing revealed that the DNA complexes fabricated from this cationic bolaamphiphile displayed marginal toxicity towards all the cell lines tested In addition, in vivo transfection studies carried out in a 4T1 mouse breast cancer model showed that the cationic bolaamphiphile delivered DNA more efficiently than PEI This cationic bolaamphiphile may make a promising gene delivery vector for future gene therapy. © 2012 Elsevier Ltd

Broad-spectrum antimicrobial supramolecular assemblies with distinctive size and shape

With the increased prevalence of antibiotic-resistant infections, there Is an urgent need for innovative antimicrobial treatments. One such area being actively explored is the use of self-assembling cationic polymers. This relatively new class of materials was inspired by biologically pervasive cationic host defense peptides. The antimicrobial action of both the synthetic polymers and naturally occurring peptides is believed to be complemented by their three-dimensional structure. In an effort to evaluate shape effects on antimicrobial materials, triblock polymers were polymerized from an assembly directing terephthalamide-bisurea core. Simple changes to this core, such as the addition of a methylene spacer, served to direct self-assembly into distinct morphologies-spheres and rods. Computational modeling also demonstrated how subtle core change! could directly alter urea stacking motifs manifesting in unique multidirectional hydrogen-bond networks despite the vast majority of material consisting of poly(lactide) (interior block) and cationic polycarbonates (exterior block). Upon testing the spherical and rod-like morphologies for antimicrobial properties, it was found that both possessed broad-spectrum activity (Gram-negative and Gram-positive bacteria as well as fungi) with minimal hemolysis, although only the rod-like assemblies were effective against Candida albicans