Environmental forcing of the Campeche cold-water coral province, southern Gulf of Mexico

With an extension of >40 km2 the recently discovered Campeche cold-water coral province located at the northeastern rim of the Campeche Bank in the southern Gulf of Mexico belongs to the largest coherent cold-water coral areas discovered so far. The Campeche province consists of numerous 20 to 40 m high coral ridges that are developed in intermediate water depths of 500 to 600 m. The ridges are colonized by a vivid cold-water coral ecosystem that covers the upper flanks and summits. The rich coral community is dominated by the framework-building scleractinia Enallopsammia profunda and Lophelia pertusa while the associated benthic megafauna shows a rather scarce occurrence. The recent environmental setting is characterized by a high surface water production caused by a local upwelling center and a dynamic bottom water regime comprising vigorous bottom currents, internal waves and strong density contrasts, which all together provide optimal conditions for the growth of cold-water corals. The strong hydrodynamics – potentially supported by the diel vertical migration of zooplankton in the Campeche area – drive the delivering of food particles to the corals. The Campeche cold-water coral province is, thus, an excellent example highlighting the importance of the hydrographic setting in securing the food supply for the development of large and vivid cold-water coral ecosystems

Origin and transformation of light hydrocarbons ascending at an active pockmark on Vestnesa Ridge, Arctic Ocean

We report on the geochemistry of hydrocarbons and pore waters down to 62.5 mbsf, collected by drilling with the MARUM‐MeBo70 and by gravity coring at the Lunde pockmark in the Vestnesa Ridge. Our data document the origin and transformations of volatiles feeding gas emissions previously documented in this region. Gas hydrates are present where a fracture network beneath the pockmark focusses migration of thermogenic hydrocarbons characterized by their C1/C2+ and stable isotopic compositions (δ2H‐CH4, δ13C‐CH4). Measured geothermal gradients (~80°C km‐1) and known formation temperatures (>70°C) suggest that those hydrocarbons are formed at depths >800 mbsf. A combined analytical/modeling approach, including concentration and isotopic mass balances, reveals that pockmark sediments experience diffuse migration of thermogenic hydrocarbons. However, at sites without channeled flow this appears to be limited to depths > ~50 mbsf. At all sites we document a contribution of microbial methanogenesis to the overall carbon cycle that includes a component of secondary carbonate reduction (CR) – i.e. reduction of dissolved inorganic carbon (DIC) generated by anaerobic oxidation of methane (AOM) in the uppermost methanogenic zone. AOM and CR rates are spatially variable within the pockmark and are highest at high‐flux sites. These reactions are revealed by δ13C‐DIC depletions at the sulfate‐methane interface at all sites. However, δ13C‐CH4 depletions are only observed at the low methane flux sites because changes in the isotopic composition of the overall methane pool are masked at high‐flux sites. 13C‐depletions of TOC suggest that at seeps sites, methane‐derived carbon is incorporated into de novo synthesized biomass

Seasonal methane accumulation and release from a gas emission site in the central North Sea

We investigated dissolved methane distributions along a 6 km transect crossing active seep sites at 40 m water depth in the central North Sea. These investigations were done under conditions of thermal stratification in summer (July 2013) and homogenous water column in winter (January 2014). Dissolved methane accumulated below the seasonal thermocline in summer with a median concentration of 390 nM, whereas during winter, methane concentrations were typically much lower (median concentration of 22 nM). High-resolution methane analysis using an underwater mass-spectrometer confirmed our summer results and was used to document prevailing stratification over the tidal cycle. We contrast estimates of methane oxidation rates (from 0.1 to 4.0 nM day⁻¹) using the traditional approach scaled to methane concentrations with microbial turnover time values and suggest that the scaling to concentration may obscure the ecosystem microbial activity when comparing systems with different methane concentrations. Our measured and averaged rate constants (k') were on the order of 0.01 day−1, equivalent to a turnover time of 100 days, even when summer stratification led to enhanced methane concentrations in the bottom water. Consistent with these observations, we could not detect known methanotrophs and pmoA genes in water samples collected during both seasons. Estimated methane fluxes indicate that horizontal transport is the dominant process dispersing the methane plume. During periods of high wind speed (winter), more methane is lost to the atmosphere than oxidized in the water. Microbial oxidation seems of minor importance throughout the year.This work is part of the DFG project “Limitations of Marine Methane Oxidation” (MA 3961/2-1). This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by Copernicus Publications on behalf of the European Geosciences Union. The published article can be found at: http://www.biogeosciences.net/ For a previous discussion paper please see: http://hdl.handle.net/1957/5749

Seasonal methane accumulation and release from a gas emission site in the central North Sea

Hydroacoustic data document the occurrence of 5 flare clusters and several single flares from which bubbles rise through the entire water column from an active seep site at 40 m water depth in the central North Sea. We investigated the difference in dissolved methane distributions along a 6 km transect crossing this seep site during a period of seasonal summer stratification (July 2013) and a period of well mixed winter water column (January 2014). Dissolved methane accumulated below the seasonal thermocline in summer with a median concentration of 390 nM, whereas during winter, methane concentrations were much lower (median concentration of 22 nM) and punctually elevated due to bubble transport. High resolution methane analysis by an underwater mass-spectrometer confirmed our summer results and were used to document prevailing stratification over the tidal cycle. Although sufficient methane was available, microbial methane oxidation was limited during both seasons. Measured and averaged rate constants (k') using Michaelis Menten kinetics were on the order of 0.01 days⁻¹, equivalent to a turnover time of 100 days. Time series measurements indicated an uptake of only 5–6% of the gas after 4 days, and no known methanotrophs and pmoA-genes were detected. Estimated methane fluxes indicate that horizontal eddy transport rapidly disperses dissolved methane, vertical transport becomes dominant during phases of high wind speeds, and relative to these processes, microbial methane oxidation appears to be comparably low. To bridge the discrete field data we developed a 1-D seasonal model using available year-long records of wind speed, surface temperature and thermocline depth. The model simulations show a peak release of methane at the beginning of fall when the water column becomes mixed. Consistent with our field data, inclusion of microbial methane oxidation does not change the model results significantly, thus microbial oxidation appears to be not sufficient to notably reduce methane during summer stratification before the peak release in fall.This work is part of the DFG project “Limitations of Marine Methane Oxidation” (MA 3961/2-1). This discussion paper has been under review for the journal Biogeosciences (BG). Please refer to the corresponding final paper in BG. The published article is copyrighted by the author(s) and published by Copernicus Publications on behalf of the European Geosciences Union. The published article can be found at: http://www.biogeosciences.net/ The final revised paper is available at: http://hdl.handle.net/1957/5749

A liver immune rheostat regulates CD8 T cell immunity in chronic HBV infection

Chronic hepatitis B virus (HBV) infection affects 300 million patients worldwide1,2, in whom virus-specific CD8 T cells by still ill-defined mechanisms lose their function and cannot eliminate HBV-infected hepatocytes3–7. Here we demonstrate that a liver immune rheostat renders virus-specific CD8 T cells refractory to activation and leads to their loss of effector functions. In preclinical models of persistent infection with hepatotropic viruses such as HBV, dysfunctional virus-specific CXCR6+ CD8 T cells accumulated in the liver and, as a characteristic hallmark, showed enhanced transcriptional activity of cAMP-responsive element modulator (CREM) distinct from T cell exhaustion. In patients with chronic hepatitis B, circulating and intrahepatic HBV-specific CXCR6+ CD8 T cells with enhanced CREM expression and transcriptional activity were detected at a frequency of 12–22% of HBV-specific CD8 T cells. Knocking out the inhibitory CREM/ICER isoform in T cells, however, failed to rescue T cell immunity. This indicates that CREM activity was a consequence, rather than the cause, of loss in T cell function, further supported by the observation of enhanced phosphorylation of protein kinase A (PKA) which is upstream of CREM. Indeed, we found that enhanced cAMP–PKA-signalling from increased T cell adenylyl cyclase activity augmented CREM activity and curbed T cell activation and effector function in persistent hepatic infection. Mechanistically, CD8 T cells recognizing their antigen on hepatocytes established close and extensive contact with liver sinusoidal endothelial cells, thereby enhancing adenylyl cyclase–cAMP–PKA signalling in T cells. In these hepatic CD8 T cells, which recognize their antigen on hepatocytes, phosphorylation of key signalling kinases of the T cell receptor signalling pathway was impaired, which rendered them refractory to activation. Thus, close contact with liver sinusoidal endothelial cells curbs the activation and effector function of HBV-specific CD8 T cells that target hepatocytes expressing viral antigens by means of the adenylyl cyclase–cAMP–PKA axis in an immune rheostat-like fashion.</p

Gridded bathymetry from EM120 multibeam echosounder of RV SONNE cruise SO222 (offshore Japan)

Bathymetry data based on multibeam echosounder (MBES) KONGSBERG EM120 was conducted during R/V SONNE cruise SO222 between 09.06.2012 and 30.06.2012 (Leg A) and 04.07.2012 and 18.07.2012 (Leg B) in the Nankai Trough off Japan. The main objective of the cruise was MeBo drilling and long-term monitoring of active mud volcanoes in the northern Kumano Basin, which carry gas hydrates and are overlying an area of high strain. Hydroacoustic work on board aimed at a high spatial coverage of the Kumano Basin and its mud volcanoes. Furthermore, bathymetric mapping revealed additional features in the study area, of which five previously unknown mud volcanoes were identified. Additionally, coring with gravity corers and the MARUM-MeBo70 drill rig as well as the deployment of CORK observation instruments and the remotely operated vehicle (ROV) MARUM-QUEST helped to study the activity and products of mud volcanoes in the Kumano Basin. CI Citation: Paul Wintersteller ([email protected]) as responsible party for bathymetry raw data ingest and approval. Description of the data source: During the SO222 cruise, the hull-mounted KONGSBERG EM120 multibeam ecosounder (MBES) was utilized for bathymetric mapping as it is able to perform in water depths up to 11,000 m. Two transducer arrays transmit acoustic signals of 11.25 to 12.6 kHz in successive emission-reception cycles. 191 overlapping beams in a 2° (TX) by 2° (RX) footprint form each acoustic ping, resulting in a beam width of maximum 150° across track and 2° along track. For further information on the system, consult https://www.km.kongsberg.com/. The depth of the water column is estimated through the two-way-travel time, beam angle and ray bending due to refraction in the water column by sound speed variations. Combining lateral and center beams secures measurement accuracy practically independent of the beam-pointing angle. During the cruise SO222, the MBES and PARASOUND sub-bottom profiler were continuously acquiring data of about 1500 km track lengths. Two sound velocity profiles (SVP) were calculated based on SEABIRD CTD measurements and applied to the MBES, whereas no SVP was available for the southern Kumano Basin due to strong currents. Responsible person during this cruise / PI: Paul Wintersteller ([email protected]) Description of data processing: Postprocessing and products were conducted by the Seafloor-Imaging & Mapping group of MARUM/FB5, responsible person: Paul Wintersteller ([email protected]). The open source software MB-System suite (Caress, D.W., and D.N. Chayes, MB-System Version 5.5, open source software distributed from the MBARI and L-DEO web sites, 2000-2012.) was utilized for this purpose. CTD measurements were carried out to conduct sound velocity profiles (SVP), which were applied during the acquisition of the hydroacoustic data. A roll correction was applied to the SO222data. Tide, pitch and heave corrections were not applied. Using Mbeditviz, artefacts were cleaned manually. NetCDF (GMT) grids of the edited data as well as statistics were created with mbgrid. The published bathymetric grids of the cruise SO222 have a resolution of 30 m. No total propagated uncertainty (TPU) has been calculated to gather vertical or horizontal accuracy. A higher resolution is, at least partly, achievable. The grid extended with _num represents a raster dataset with the statistical number of beams/depths taken into account to create the depth of the cell. The extended _sd -grid contains the standard deviation for each cell. All grids produced are retrievable through the PANGAEA database (www.pangaea.de). Projection: Geographic Coordinate System / WGS84 Chief Scientist: Achim Kopf ([email protected]) CR: http://elib.suub.uni-bremen.de/edocs/00103543-1.pdf CSR: https://www2.bsh.de/aktdat/dod/fahrtergebnis/2012/20120280.htm Raw data: https://doi.pangaea.de/10.1594/PANGAEA.90124