User\u27s guide to understanding audits and auditor\u27s reports

https://egrove.olemiss.edu/aicpa_guides/1759/thumbnail.jp

Let\u27s change GAAS!!! ???*&#@

https://egrove.olemiss.edu/dl_proceedings/1151/thumbnail.jp

Practical guidance for implementing SSARS 8 : how to understand and apply the amendments to SSARS 1; Compilation and review alert;Audit risk alerts

https://egrove.olemiss.edu/aicpa_indev/1714/thumbnail.jp

Evolution of Audit reporting

https://egrove.olemiss.edu/dl_proceedings/1132/thumbnail.jp

Internal control: Progress and perils

https://egrove.olemiss.edu/dl_proceedings/1093/thumbnail.jp

Auditing for fraud: Perception vs. reality

https://egrove.olemiss.edu/dl_proceedings/1046/thumbnail.jp

Compilation and Review Manual, Volume 2

https://egrove.olemiss.edu/aicpa_guides/1846/thumbnail.jp

Compilation and Review Manual, Volume 1

https://egrove.olemiss.edu/aicpa_guides/1845/thumbnail.jp

A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery

RNA-based therapies have great potential to treat many undruggable human diseases. However, their efficacy, in particular for mRNA, remains hampered by poor cellular delivery and limited endosomal escape. Development and optimisation of delivery vectors, such as lipid nanoparticles (LNPs), are impeded by limited screening methods to probe the intracellular processing of LNPs in sufficient detail. We have developed a high-throughput imaging-based endosomal escape assay utilising a Galectin-9 reporter and fluorescently labelled mRNA to probe correlations between nanoparticle-mediated uptake, endosomal escape frequency, and mRNA translation. Furthermore, this assay has been integrated within a screening platform for optimisation of lipid nanoparticle formulations. We show that Galectin-9 recruitment is a robust, quantitative reporter of endosomal escape events induced by different mRNA delivery nanoparticles and small molecules. We identify nanoparticles with superior escape properties and demonstrate cell line variances in endosomal escape response, highlighting the need for fine-tuning of delivery formulations for specific applications