Different populations of RNA polymerase II in living mammalian cells

RNA polymerase II is responsible for transcription of most eukaryotic genes, but, despite exhaustive analysis, little is known about how it transcribes natural templates in vivo. We studied polymerase dynamics in living Chinese hamster ovary cells using an established line that expresses the largest (catalytic) subunit of the polymerase (RPB1) tagged with the green fluorescent protein (GFP). Genetic complementation has shown this tagged polymerase to be fully functional. Fluorescence loss in photobleaching (FLIP) reveals the existence of at least three kinetic populations of tagged polymerase: a large rapidly-exchanging population, a small fraction resistant to 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB) but sensitive to a different inhibitor of transcription (i.e. heat shock), and a third fraction sensitive to both inhibitors. Quantitative immunoblotting shows the largest fraction to be the inactive hypophosphorylated form of the polymerase (i.e. IIA). Results are consistent with the second (DRB-insensitive but heat-shock-sensitive) fraction being bound but not engaged, while the third (sensitive to both DRB and heat shock) is the elongating hyperphosphorylated form (i.e. IIO)

Regular and Black Hole Solutions in the Einstein-Skyrme Theory with Negative Cosmological Constant

We study spherically symmetric regular and black hole solutions in the Einstein-Skyrme theory with a negative cosmological constant. The Skyrme field configuration depends on the value of the cosmological constant in a similar manner to effectively varying the gravitational constant. We find the maximum value of the cosmological constant above which there exists no solution. The properties of the solutions are discussed in comparison with the asymptotically flat solutions. The stability is investigated in detail by solving the linearly perturbed equation numerically. We show that there exists a critical value of the cosmological constant above which the solution in the branch representing unstable configuration in the asymptotically flat spacetime turns to be linearly stable.Comment: 10 pages, 9 figures, comments and one reference added, to appear in Class.Quant.Gra

Abelian Higgs Hair for AdS-Schwarzschild Black Hole

We show that the Abelian Higgs field equations in the background of the four dimensional AdS-Schwarzschild black hole have a vortex line solution. This solution, which has axial symmetry, is a generalization of the AdS spacetime Nielsen-Olesen string. By a numerical study of the field equations, we show that black hole could support the Abelian Higgs field as its Abelian hair. Also, we conside the self gravity of the Abelian Higgs field both in the pure AdS spacetime and AdS-Schwarzschild black hole background and show that the effect of string as a black hole hair is to induce a deficit angle in the AdS-Schwarzschild black hole.Comment: 19 pages, 33 figure

Gravitating sphalerons and sphaleron black holes in asymptotically anti-de Sitter spacetime

Numerical arguments are presented for the existence of spherically symmetric regular and black hole solutions of the EYMH equations with a negative cosmological constant. These solutions approach asymptotically the anti-de Sitter spacetime. The main properties of the solutions and the differences with respect to the asymptotically flat case are discussed. The instability of the gravitating sphaleron solutions is also proven.Comment: 30 pages, LaTeX, 8 Encapsulated PostScript figure

Abelian Higgs Hair for a Static Charged Black String

We study the problem of vortex solutions in the background of an electrically charged black string. We show numerically that the Abelian Higgs field equations in the background of a four-dimensional black string have vortex solutions. These solutions which have axial symmetry, show that the black string can support the Abelian Higgs field as hair. This situation holds also in the case of the extremal black string. We also consider the self-gravity of the Abelian Higgs field and show that the effect of the vortex is to induce a deficit angle in the metric under consideration.Comment: REVTEX4, 12 pages, 6 figures, The version to be appeared in Phys. Rev.

New hairy black hole solutions with a dilaton potential

We consider black hole solutions with a dilaton field possessing a nontrivial potential approaching a constant negative value at infinity. The asymptotic behaviour of the dilaton field is assumed to be slower than that of a localized distribution of matter. A nonabelian SU(2) gauge field is also included in the total action. The mass of the solutions admitting a power series expansion in $1/r$ at infinity and preserving the asymptotic anti-de Sitter geometry is computed by using a counterterm subtraction method. Numerical arguments are presented for the existence of hairy black hole solutions for a dilaton potential of the form $V(\phi)=C_1 \exp(2\alpha_1 \phi)+C_2 \exp(2\alpha_2 \phi)+C_3$, special attention being paid to the case of ${\cal N}=4, D=4$ gauged supergravity model of Gates and Zwiebach.Comment: 12 pages, 4 figures; v2:references added, typos corrected, small changes in Section

Quasi-normal modes of the scalar hairy black hole

We calculate QNMs of the scalar hairy black hole in the AdS background using Horowitz-Hubeny method for the potential that is not known in analytical form. For some black hole parameters we found pure imaginary frequencies. Increasing of the scalar field mass does not cause the imaginary part to vanish, it reaches some minimum and then increases, thus in the case under consideration the infinitely long living modes (quasi-resonances) do not appear.Comment: 17 pages, 17 figures, LaTe

Quasi-normal modes of toroidal, cylindrical and planar black holes in anti-de Sitter spacetimes: scalar, electromagnetic and gravitational perturbations

We study the quasi-normal modes (QNM) of scalar, electromagnetic and gravitational perturbations of black holes in general relativity whose horizons have toroidal, cylindrical or planar topology in an asymptotically anti-de Sitter (AdS) spacetime. The associated quasinormal frequencies describe the decay in time of the corresponding test field in the vicinities of the black hole. In terms of the AdS/CFT conjecture, the inverse of the frequency is a measure of the dynamical timescale of approach to thermal equilibrium of the corresponding conformal field theory.Comment: Latex, 16 pages. Minor change

Global behavior of solutions to the static spherically symmetric EYM equations

The set of all possible spherically symmetric magnetic static Einstein-Yang-Mills field equations for an arbitrary compact semi-simple gauge group $G$ was classified in two previous papers. Local analytic solutions near the center and a black hole horizon as well as those that are analytic and bounded near infinity were shown to exist. Some globally bounded solutions are also known to exist because they can be obtained by embedding solutions for the $G=SU(2)$ case which is well understood. Here we derive some asymptotic properties of an arbitrary global solution, namely one that exists locally near a radial value $r_{0}$, has positive mass $m(r)$ at $r_{0}$ and develops no horizon for all $r>r_{0}$. The set of asymptotic values of the Yang-Mills potential (in a suitable well defined gauge) is shown to be finite in the so-called regular case, but may form a more complicated real variety for models obtained from irregular rotation group actions.Comment: 43 page