Deep Sequencing Analysis Reveals the Mycoviral Diversity of the Virome of an Avirulent Isolate of Rhizoctonia solani AG-2-2 IV

Bartholomaeus A, Wibberg D, Winkler A, Pühler A, Schlüter A, Varrelmann M. Deep Sequencing Analysis Reveals the Mycoviral Diversity of the Virome of an Avirulent Isolate of Rhizoctonia solani AG-2-2 IV. PLOS ONE. 2016;11(11): e0165965.Rhizoctonia solani represents an important plant pathogenic Basidiomycota species complex and the host of many different mycoviruses, as indicated by frequent detection of dsRNA elements in natural populations of the fungus. To date, eight different mycoviruses have been characterized in Rhizoctonia and some of them have been reported to modulate its virulence. DsRNA extracts of the avirulent R. solani isolate DC17 (AG2-2-IV) displayed a diverse pattern, indicating multiple infections with mycoviruses. Deep sequencing analysis of the dsRNA extract, converted to cDNA, revealed that this isolate harbors at least 17 different mycovirus species. Based on the alignment of the conserved RNA-dependent RNApolymerase (RdRp) domain, this viral community included putative members of the families Narnaviridae, Endornaviridae, Partitiviridae and Megabirnaviridae as well as of the order Tymovirales. Furthermore, viruses, which could not be assigned to any existing family or order, but showed similarities to so far unassigned species like Sclerotinia sclerotiorum RNA virus L, Rhizoctonia solani dsRNA virus 1, Aspergillus foetidus slow virus 2 or Rhizoctonia fumigata virus 1, were identified. This is the first report of a fungal isolate infected by 17 different viral species and a valuable study case to explore the diversity of mycoviruses infecting R. solani

Complete Genome Sequence of the Type Strain Corynebacterium Mustelae DSM 45274, Isolated from Various Tissues of a Male Ferret with Lethal Sepsis

The complete genome of Corynebacterium mustelae DSM 45274 comprises 3,474,226 bp and 3,188 genes. Prominent niche and virulence factors are SpaBCA- and SpaDEF-type pili with similarity to pilus proteins of Corynebacterium resistens and Corynebacterium urealyticum and an immunomodulatory EndoS-like endoglycosidase probably catalyzing the removal of distinct glycans from IgG antibodies

Complete Genome Sequence of the Type Strain Corynebacterium Epidermidicanis DSM 45586, Isolated from the Skin of a Dog Suffering from Pruritus

The complete genome sequence of Corynebacterium epidermidicanis DSM 45586 comprises 2,692,072 bp with 58.06% G+C content. The annotation revealed 2,466 protein-coding regions, including genes for surface-anchored proteins with Cna B-type or bacterial Ig-like domains and for an adhesive SpaABC-type pilus with similarity to fimbrial subunits of Corynebacterium resistens DSM 45100

Complete Genome Sequence of the Type Strain Corynebacterium mustelae DSM 45274, Isolated from Various Tissues of a Male Ferret with Lethal Sepsis

Rückert C, Eimer J, Winkler A, Tauch A. Complete Genome Sequence of the Type Strain Corynebacterium mustelae DSM 45274, Isolated from Various Tissues of a Male Ferret with Lethal Sepsis. Genome announcements. 2015;3(5): e01012-15.The complete genome of Corynebacterium mustelae DSM 45274 comprises 3,474,226 bp and 3,188 genes. Prominent niche and virulence factors are SpaBCA- and SpaDEF-type pili with similarity to pilus proteins of Corynebacterium resistens and Corynebacterium urealyticum and an immunomodulatory EndoS-like endoglycosidase probably catalyzing the removal of distinct glycans from IgG antibodies

Complete Genome Sequence of the Type Strain Corynebacterium epidermidicanis DSM 45586, Isolated from the Skin of a Dog Suffering from Pruritus

Rückert C, Eimer J, Winkler A, Tauch A. Complete Genome Sequence of the Type Strain Corynebacterium epidermidicanis DSM 45586, Isolated from the Skin of a Dog Suffering from Pruritus. Genome announcements. 2015;3(4): e00959-15.The complete genome sequence of Corynebacterium epidermidicanis DSM 45586 comprises 2,692,072 bp with 58.06% G+C content. The annotation revealed 2,466 protein-coding regions, including genes for surface-anchored proteins with Cna B-type or bacterial Ig-like domains and for an adhesive SpaABC-type pilus with similarity to fimbrial subunits of Corynebacterium resistens DSM 45100

Complete Genome Sequence of Corynebacterium camporealensis DSM 44610, Isolated from the Milk of a Manchega Sheep with Subclinical Mastitis

Rückert C, Albersmeier A, Winkler A, Tauch A. Complete Genome Sequence of Corynebacterium camporealensis DSM 44610, Isolated from the Milk of a Manchega Sheep with Subclinical Mastitis. Genome announcements. 2015;3(3): e00572-15.Corynebacterium camporealensis has been isolated in pure culture from milk samples of dairy sheep affected by subclinical mastitis. The complete genome sequence of the type strain DSM 44610, recovered from milk of a Manchega sheep, comprises 2,451,810 bp with a mean G+C content of 59.41% and 2,249 protein-coding genes

Complete Genome Sequence of the Type Strain Corynebacterium testudinoris DSM 44614, Recovered from Necrotic Lesions in the Mouth of a Tortoise

The complete genome sequence of the type strain Corynebacterium testudinoris DSM 44614 from the mouth of a tortoise comprises 2,721,226 bp with a mean G+C content of 63.14%. The automatic annotation of the genome sequence revealed 4 rRNA operons, 51 tRNA genes, 7 other RNA genes, and 2,561 protein-coding regions.Medical Microbiology and Genomics fund (eKVV 200937)Germany. Federal Ministry of Education and Research (German Network for Bioinformatics Intrastructure Initiative FKZ 031A533A

Virulence Factor Genes Detected in the Complete Genome Sequence of Corynebacterium uterequi DSM 45634, Isolated from the Uterus of a Maiden Mare

The complete genome sequence of the type strain Corynebacterium uterequi DSM 45634 from an equine urogenital tract specimen comprises 2,419,437 bp and 2,163 protein-coding genes. Candidate virulence factors are homologs of DIP0733, DIP1281, and DIP1621 from Corynebacterium diphtheriae and of sialidase precursors from Trueperella pyogenes and Chlamydia trachomatis.Medical Microbiology and Genomics fund (eKVV 200937)Germany. Federal Ministry of Education and Research (German Network for Bioinformatics Intrastructure Initiative FKZ 031A533A

Complete Genome Sequence ofAcinetobacter baumanniiCIP 70.10, a Susceptible Reference Strain for Comparative Genome Analyses

Krahn T, Wibberg D, Maus I, et al. Complete Genome Sequence ofAcinetobacter baumanniiCIP 70.10, a Susceptible Reference Strain for Comparative Genome Analyses. Genome Announcements. 2015;3(4):e00850-15