Bovine oviductal and embryonic insulin-like growth factor binding proteins: possible regulators of embryotrophic insulin-like growth factor circuits.

Bovine oviductal monolayer and vesicle primary cultures express insulin-like growth factor (IGF)-I and -II mRNAs and polypeptides. Early bovine embryos also express IGF-I, IGF-II, IGF-I receptor, IGF-II receptor, and insulin receptor mRNAs. This study reports the expression of IGF binding protein (IGFBP) mRNAs and polypeptides in bovine oviduct primary cultures and IGFBP mRNAs in preattachment embryos. Release of immunoreactive IGF-I and IGF-II by oviduct cultures and bovine blastocysts was also determined. IGFBP-2, -3, -4, and -5 transcripts were observed in oviduct primary cultures throughout an 8-day interval. IGFBP-1 and -6 mRNAs were consistently not detected in the oviduct. Messenger RNAs encoding IGFBPs -2, -3, and -4 were detected throughout bovine preattachment development, while transcripts encoding IGFBP-5 were detected only in blastocysts. IGFBP-1 and -6 transcripts were not detected in early embryos. Ligand blot analysis with 125I-labeled IGF-II revealed the presence of four prominent polypeptide bands of approximate molecular masses 24, 31, and 36 kDa, and a broad band extending from 46 to 53 kDa, in conditioned media samples prepared from oviduct primary cultures. Western immunoblot analysis confirmed the identity of the 24-kDa, 31-kDa, and 36-kDa species as IGFBP-4, -5, and -2, respectively. Levels of the release of IGF-II from oviductal vesicle cultures were significantly greater than levels observed for monolayer cultures (p \u3c 0.005). No significant difference in the levels of IGF-I release between monolayer and vesicle cultures was observed. Pools of 10 blastocysts released on average 36.2 +/- 3.9 pg of IGF-II per embryo, while the release of embryonic IGF-I was below the levels of detection for our assay. The results suggest that maternally derived IGF may be regulated by IGFBPs to support bovine preattachment development

Beta decay of 71,73Co; probing single particle states approaching doubly magic 78Ni

Low-energy excited states in 71,73Ni populated via the {\beta} decay of 71,73Co were investigated in an experiment performed at the National Superconducting Cyclotron Laboratory (NSCL) at Michigan State University (MSU). Detailed analysis led to the construction of level schemes of 71,73Ni, which are interpreted using systematics and analyzed using shell model calculations. The 5/2- states attributed to the the f5/2 orbital and positive parity 5/2+ and 7/2+ states from the g9/2 orbital have been identified in both 71,73Ni. In 71Ni the location of a 1/2- {\beta}-decaying isomer is proposed and limits are suggested as to the location of the isomer in 73Ni. The location of positive parity cluster states are also identified in 71,73Ni. Beta-delayed neutron branching ratios obtained from this data are given for both 71,73Co.Comment: Accepted for publication in PR

(Micro)evolutionary changes and the evolutionary potential of bird migration

Seasonal migration is the yearly long-distance movement of individuals between their breeding and wintering grounds. Individuals from nearly every animal group exhibit this behavior, but probably the most iconic migration is carried out by birds, from the classic V-shape formation of geese on migration to the amazing nonstop long-distance flights undertaken by Arctic Terns Sterna paradisaea. In this chapter, we discuss how seasonal migration has shaped the field of evolution. First, this behavior is known to turn on and off quite rapidly, but controversy remains concerning where this behavior first evolved geographically and whether the ancestral state was sedentary or migratory (Fig. 7.1d, e). We review recent work using new analytical techniques to provide insight into this topic. Second, it is widely accepted that there is a large genetic basis to this trait, especially in groups like songbirds that migrate alone and at night precluding any opportunity for learning. Key hypotheses on this topic include shared genetic variation used by different populations to migrate and only few genes being involved in its control. We summarize recent work using new techniques for both phenotype and genotype characterization to evaluate and challenge these hypotheses. Finally, one topic that has received less attention is the role these differences in migratory phenotype could play in the process of speciation. Specifically, many populations breed next to one another but take drastically different routes on migration (Fig. 7.2). This difference could play an important role in reducing gene flow between populations, but our inability to track most birds on migration has so far precluded evaluations of this hypothesis. The advent of new tracking techniques means we can track many more birds with increasing accuracy on migration, and this work has provided important insight into migration's role in speciation that we will review here

Proton Drip-Line Calculations and the Rp-process

One-proton and two-proton separation energies are calculated for proton-rich nuclei in the region $A=41-75$. The method is based on Skyrme Hartree-Fock calculations of Coulomb displacement energies of mirror nuclei in combination with the experimental masses of the neutron-rich nuclei. The implications for the proton drip line and the astrophysical rp-process are discussed. This is done within the framework of a detailed analysis of the sensitivity of rp process calculations in type I X-ray burst models on nuclear masses. We find that the remaining mass uncertainties, in particular for some nuclei with $N=Z$, still lead to large uncertainties in calculations of X-ray burst light curves. Further experimental or theoretical improvements of nuclear mass data are necessary before observed X-ray burst light curves can be used to obtain quantitative constraints on ignition conditions and neutron star properties. We identify a list of nuclei for which improved mass data would be most important.Comment: 20 pages, 9 figures, 2 table

β -delayed neutron emission from Ga 85

Decay of Ga85 was studied by means of β-neutron-γ spectroscopy. A pure beam of Ga85 was produced at the Holifield Radioactive Ion Beam Facility using a resonance ionization laser ion source and a high-resolution electromagnetic separator. The β-delayed neutron emission probability was measured for the first time, yielding 70(5)%. An upper limit of 0.1% for β-delayed two-neutron emission was also experimentally established for the first time. A detailed decay scheme including absolute γ-ray intensities was obtained. Results are compared with theoretical β-delayed emission models

β-decay studies of the transitional nucleus Cu75 and the structure of Zn75

The β decay of Cu75 [t1/2=1.222(8)s] to levels in Zn75 was studied at the Holifield Radioactive Ion Beam Facility of Oak Ridge National Laboratory. The γγ and βγ data were collected at the Low-energy Radioactive Ion Beam Spectroscopy Station using the high-resolution isobar separator to obtain a purified Cu75 beam with a rate of over 2000 ions per second. The excited states in Zn75 have been identified for the first time. A total of 120 γ-ray transitions were placed in a level scheme containing 59 levels including two states above the neutron separation energy and a previously unknown 1/2- isomeric state at 127 keV. Spins and parities of several states were deduced and interpreted based on the observed β feeding and γ-decay pattern. © 2011 American Physical Society

Excited states in As 82 studied in the decay of Ge 82

The excited states of odd-odd As82 are studied in the β decay of Ge82. An isotopically pure beam of Ga83 was produced at the Holifield Radioactive Ion Beam Facility using a resonance ionization laser ion source and high-resolution electromagnetic separation. The atoms of Ge82 are created after β-delayed neutron emission in the decay of Ga83. The number of Ge82 atoms is found by normalization to the 1348-keV γ ray. Detailed analysis of the decay scheme is compared with shell-model calculations with several commonly used fpg shell interactions

βdecays of \u3csup\u3e92\u3c/sup\u3eRb, \u3csup\u3e96gs\u3c/sup\u3eY, and \u3csup\u3e142\u3c/sup\u3eCs measured with the modular total absorption spectrometer and the influence of multiplicity on total absorption spectrometry measurements

Total absorption spectroscopy is a technique that helps obtain reliable β-feeding patterns of complex decays important for nuclear structure and astrophysics modeling as well as decay heat analysis in nuclear reactors. The need for improved measurements of β-feeding patterns from fission decay products has come to the forefront of experiments that use nuclear reactors as a source of antineutrinos. Here we present more detailed results, in particular the β-decay measurements of 96gsY, and demonstrate the impact of the β-delayed γ multiplicity on the overall efficiency of Modular Total Absorption Spectrometer used at Oak Ridge National Laboratory to study the decays of fission products abundant during a nuclear fuel cycle

β -decay study of Kr 94

β decay of neutron-rich nuclide Kr94 was reinvestigated by means of a high resolution on-line mass separator and β-γ spectroscopy. In total 22 γ-ray transitions were assigned to the decay of Kr94, and a new isomeric state was identified. The new information allows us to build detailed levels systematics in a chain of odd-odd rubidium isotopes and draw conclusions on nuclear structure for some of the observed states. The discussed level structure affects the evolution of β-decay half-lives for neutron-rich selenium, krypton, and strontium isotopes

β-Decay study of neutron-rich bromine and krypton isotopes

Short-lived neutron-rich nuclei including 93Br, 93Kr, and 94Kr were produced in proton-induced fission of 238U at the Holifield Radioactive Ion Beam Facility in Oak Ridge. Their β decay was studied by means of a high-resolution online mass separator and β-γ spectroscopy methods. The half-life of T1/2 = 152(8) ms and β-delayed branching ratio of Pn = 53-8+11% measured for 93Br differs from the previously reported values of T1/2 = 102(10) ms and Pn = 68(7)%. At the same time the half-life of 94Kr T1/2 = 227(14) ms and both the half-life of T1/2 = 1.298(54) s and β-delayed branching ratio of Pn = 1.9-0.2+0.6% of 93Kr are in very good agreement with literature values. The decay properties of 93Br include previously unreported γ transitions following β-delayed neutron emission. © 2013 American Physical Society