Sub-m s−1^{-1} upper limits from a deep HARPS-N radial-velocity search for planets orbiting HD 166620 and HD 144579

Minimising the impact of stellar variability in Radial Velocity (RV) measurements is a critical challenge in achieving the 10 cm s$^{-1}$ precision needed to hunt for Earth twins. Since 2012, a dedicated programme has been underway with HARPS-N, to conduct a blind RV Rocky Planets Search (RPS) around bright stars in the Northern Hemisphere. Here we describe the results of a comprehensive search for planetary systems in two RPS targets, HD 166620 and HD 144579. Using wavelength-domain line-profile decorrelation vectors to mitigate the stellar activity and performing a deep search for planetary reflex motions using a trans-dimensional nested sampler, we found no significant planetary signals in the data sets of either of the stars. We validated the results via data-splitting and injection recovery tests. Additionally, we obtained the 95th percentile detection limits on the HARPS-N RVs. We found that the likelihood of finding a low-mass planet increases noticeably across a wide period range when the inherent stellar variability is corrected for using scalpels U-vectors. We are able to detect planet signals with $M\sin i \leq 1$ M$_\oplus$ for orbital periods shorter than 10 days. We demonstrate that with our decorrelation technique, we are able to detect signals as low as 54 cm s$^{-1}$, which brings us closer to the calibration limit of 50 cm s$^{-1}$ demonstrated by HARPS-N. Therefore, we show that we can push down towards the RV precision required to find Earth analogues using high-precision radial velocity data with novel data-analysis techniques.Comment: 7 tables, 24 figures (including those in appendix

Noise Sources in Photometry and Radial Velocities

The quest for Earth-like, extrasolar planets (exoplanets), especially those located inside the habitable zone of their host stars, requires techniques sensitive enough to detect the faint signals produced by those planets. The radial velocity (RV) and photometric transit methods are the most widely used and also the most efficient methods for detecting and characterizing exoplanets. However, presence of astrophysical "noise" makes it difficult to detect and accurately characterize exoplanets. It is important to note that the amplitude of such astrophysical noise is larger than both the signal of Earth-like exoplanets and state-of-the-art instrumentation limit precision, making this a pressing topic that needs to be addressed. In this chapter, I present a general review of the main sources of noise in photometric and RV observations, namely, stellar oscillations, granulation, and magnetic activity. Moreover, for each noise source I discuss the techniques and observational strategies which allow us to mitigate their impact.Comment: 11 pages, 2 tables, Lecture presented at the IVth Azores International Advanced School in Space Sciences on "Asteroseismology and Exoplanets: Listening to the Stars and Searching for New Worlds" (arXiv:1709.00645), which took place in Horta, Azores Islands, Portugal in July 201

Clinical safety of dichlorvos (45%), cypermethrin (5%) and piperonyl butoxide (25%) administered by spray on the skin of cattle

ABSTRACT Objective. Due to the importance of controlling ectoparasites, associated with the necessity of technical knowledge on the safety of topical treatment with organophosphates, pyrethroids and piperonyl butoxide to the animal organism, this bioassay was carried out to evaluate the clinical safety of the association of dichlorvos (45%) + cypermethrin (5%) + piperonyl butoxide (25%) administered by spray on the skin of cattle, through the study of clinical parameters, biochemical, haematological and behavioral changes. Materials and methods. Sixteen crossbred animals with a mean age of 18 months, males and females grouped into two treatments with eight animals each: T1 (1:800 v/v) and T2 (1:200 v/v). Were collected blood samples at six different times: before treatment (BT), 24, 48, 72, 96 and 192 hours post treatment (HPT). Results. The antiparasitic association administered by spray on the skin did not result in changes in the enzymatic activity of ALT, AST, GGT and ALP, as well as in serum albumin, triglycerides, cholesterol, urea and creatinine, demonstrating the safety of this antiparasitic compound for maintaining hepatic and renal functionality. The erythrocyte, leukocyte and platelet studies showed no changes caused by treatments, and no clinical signs and behavioral changes were observed after treatment. Conclusions. These findings demonstrated good safety margin for spray treatment on the skin with this antiparasitic compound, even when administered at a dilution of 1:200 v/v, which is four times the dose recommended for ectoparasite control

A one-step low-cost molecular test for SARS-CoV-2 detection suitable for community testing using minimally processed saliva

Funding Information: This work was supported by (i) Project LISBOA-01-0145-FEDER-007660 (\u2018Microbiologia Molecular, Estrutural e Celular\u2019) funded by FEDER \u2018Fundo Europeu de Desenvolvimento Regional\u2019 funds through COMPETE2020\u2014\u2018Programa Operacional Competitividade e Internacionaliza\u00E7\u00E3o\u2019 (POCI), (ii) \u2018Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia\u2019 (FCT) through the project DETECT Ref 433_613549914 (20/7/153), under the scope of the 2nd edition of the programme RESEARCH4COVID19, (iii) the project \u2018STOP-COVID\u2014Strategies to prevent COVID-19 by early detection of asymptomatic carriers at increased risk: epidemiological studies and validation of a rapid in-house diagnostic test\u2019, Ref 072559, funded by FEDER from \u2018Programa Operacional Regional Lisboa\u2019 and (iv) the Municipality of Oeiras. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Funding Information: This work was supported by (i) Project LISBOA-01-0145-FEDER- 007660 ( Microbiologia Molecular, Estrutural e Celular ) funded by FEDER Fundo Europeu de Desenvolvimento Regional funds through COMPETE2020\u2014 Programa Operacional Competitividade e Internacionaliza\u00E7ao ~ (POCI), (ii) Funda\u00E7ao ~ para a Ciencia ^ e a Tecnologia (FCT) through the project DETECT Ref 433_613549914 (20/7/153), under the scope of the 2nd edition of the programme RESEARCH4COVID19, (iii) the project STOP-COVID\u2014Strategies to prevent COVID-19 by early detection of asymptomatic carriers at increased risk: epidemiological studies and validation of a rapid in-house diagnostic test , Ref 072559, funded by FEDER from Programa Operacional Regional Lisboa and (iv) the Municipality of Oeiras. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Publisher Copyright: © 2024 The Author(s). Published by Oxford University Press.The gold standard for coronavirus disease 2019 diagnostic testing relies on RNA extraction from naso/oropharyngeal swab followed by amplification through reverse transcription-polymerase chain reaction (RT-PCR) with fluorogenic probes. While the test is extremely sensitive and specific, its high cost and the potential discomfort associated with specimen collection made it suboptimal for public health screening purposes. In this study, we developed an equally reliable, but cheaper and less invasive alternative test based on a one-step RT-PCR with the DNA-intercalating dye SYBR Green, which enables the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) directly from saliva samples or RNA isolated from nasopharyngeal (NP) swabs. Importantly, we found that this type of testing can be fine-tuned to discriminate SARS-CoV-2 variants of concern. The saliva RT-PCR SYBR Green test was successfully used in a mass-screening initiative targeting nearly 4500 asymptomatic children under the age of 12. Testing was performed at a reasonable cost, and in some cases, the saliva test outperformed NP rapid antigen tests in identifying infected children. Whole genome sequencing revealed that the antigen testing failure could not be attributed to a specific lineage of SARS-CoV-2. Overall, this work strongly supports the view that RT-PCR saliva tests based on DNA-intercalating dyes represent a powerful strategy for community screening of SARS-CoV-2. The tests can be easily applied to other infectious agents and, therefore, constitute a powerful resource for an effective response to future pandemics.publishe

Markedly Divergent Tree Assemblage Responses to Tropical Forest Loss and Fragmentation across a Strong Seasonality Gradient

We examine the effects of forest fragmentation on the structure and composition of tree assemblages within three seasonal and aseasonal forest types of southern Brazil, including evergreen, Araucaria, and deciduous forests. We sampled three southernmost Atlantic Forest landscapes, including the largest continuous forest protected areas within each forest type. Tree assemblages in each forest type were sampled within 10 plots of 0.1 ha in both continuous forests and 10 adjacent forest fragments. All trees within each plot were assigned to trait categories describing their regeneration strategy, vertical stratification, seed-dispersal mode, seed size, and wood density. We detected differences among both forest types and landscape contexts in terms of overall tree species richness, and the density and species richness of different functional groups in terms of regeneration strategy, seed dispersal mode and woody density. Overall, evergreen forest fragments exhibited the largest deviations from continuous forest plots in assemblage structure. Evergreen, Araucaria and deciduous forests diverge in the functional composition of tree floras, particularly in relation to regeneration strategy and stress tolerance. By supporting a more diversified light-demanding and stress-tolerant flora with reduced richness and abundance of shade-tolerant, old-growth species, both deciduous and Araucaria forest tree assemblages are more intrinsically resilient to contemporary human-disturbances, including fragmentation-induced edge effects, in terms of species erosion and functional shifts. We suggest that these intrinsic differences in the direction and magnitude of responses to changes in landscape structure between forest types should guide a wide range of conservation strategies in restoring fragmented tropical forest landscapes worldwide

Complementarity of Spike- and Rate-Based Dynamics of Neural Systems

Relationships between spiking-neuron and rate-based approaches to the dynamics of neural assemblies are explored by analyzing a model system that can be treated by both methods, with the rate-based method further averaged over multiple neurons to give a neural-field approach. The system consists of a chain of neurons, each with simple spiking dynamics that has a known rate-based equivalent. The neurons are linked by propagating activity that is described in terms of a spatial interaction strength with temporal delays that reflect distances between neurons; feedback via a separate delay loop is also included because such loops also exist in real brains. These interactions are described using a spatiotemporal coupling function that can carry either spikes or rates to provide coupling between neurons. Numerical simulation of corresponding spike- and rate-based methods with these compatible couplings then allows direct comparison between the dynamics arising from these approaches. The rate-based dynamics can reproduce two different forms of oscillation that are present in the spike-based model: spiking rates of individual neurons and network-induced modulations of spiking rate that occur if network interactions are sufficiently strong. Depending on conditions either mode of oscillation can dominate the spike-based dynamics and in some situations, particularly when the ratio of the frequencies of these two modes is integer or half-integer, the two can both be present and interact with each other

Entomopathogenic Fungus as a Biological Control for an Important Vector of Livestock Disease: The Culicoides Biting Midge

BACKGROUND: The recent outbreak of bluetongue virus in northern Europe has led to an urgent need to identify control measures for the Culicoides (Diptera: Ceratopogonidae) biting midges that transmit it. Following successful use of the entomopathogenic fungus Metarhizium anisopliae against larval stages of biting midge Culicoides nubeculosus Meigen, we investigated the efficacy of this strain and other fungi (Beauveria bassiana, Isaria fumosorosea and Lecanicillium longisporum) as biocontrol agents against adult C. nubeculosus in laboratory and greenhouse studies. METHODOLOGY/FINDINGS: Exposure of midges to 'dry' conidia of all fungal isolates caused significant reductions in survival compared to untreated controls. Metarhizium anisopliae strain V275 was the most virulent, causing a significantly decrease in midge survival compared to all other fungal strains tested. The LT(50) value for strain V275 was 1.42 days compared to 2.21-3.22 days for the other isolates. The virulence of this strain was then further evaluated by exposing C. nubeculosus to varying doses (10(8)-10(11) conidia m(-2)) using different substrates (horse manure, damp peat, leaf litter) as a resting site. All exposed adults were found to be infected with the strain V275 four days after exposure. A further study exposed C. nubeculosus adults to 'dry' conidia and 'wet' conidia (conidia suspended in 0.03% aq. Tween 80) of strain V275 applied to damp peat and leaf litter in cages within a greenhouse. 'Dry' conidia were more effective than 'wet' conidia, causing 100% mortality after 5 days. CONCLUSION/SIGNIFICANCE: This is the first study to demonstrate that entomopathogenic fungi are potential biocontrol agents against adult Culicoides, through the application of 'dry' conidia on surfaces (e.g., manure, leaf litter, livestock) where the midges tend to rest. Subsequent conidial transmission between males and females may cause an increased level of fungi-induced mortality in midges thus reducing the incidence of disease

Measurements of Higgs boson production and couplings in diboson final states with the ATLAS detector at the LHC

Measurements are presented of production properties and couplings of the recently discovered Higgs boson using the decays into boson pairs, H →γ γ, H → Z Z∗ →4l and H →W W∗ →lνlν. The results are based on the complete pp collision data sample recorded by the ATLAS experiment at the CERN Large Hadron Collider at centre-of-mass energies of √s = 7 TeV and √s = 8 TeV, corresponding to an integrated luminosity of about 25 fb−1. Evidence for Higgs boson production through vector-boson fusion is reported. Results of combined fits probing Higgs boson couplings to fermions and bosons, as well as anomalous contributions to loop-induced production and decay modes, are presented. All measurements are consistent with expectations for the Standard Model Higgs boson

Standalone vertex finding in the ATLAS muon spectrometer

A dedicated reconstruction algorithm to find decay vertices in the ATLAS muon spectrometer is presented. The algorithm searches the region just upstream of or inside the muon spectrometer volume for multi-particle vertices that originate from the decay of particles with long decay paths. The performance of the algorithm is evaluated using both a sample of simulated Higgs boson events, in which the Higgs boson decays to long-lived neutral particles that in turn decay to bbar b final states, and pp collision data at √s = 7 TeV collected with the ATLAS detector at the LHC during 2011