Fatty acid composition of total lipids in A) seed oil, B) pulp oil and C) whole berry oil of four cultivars RC-4, E6590, FR-14 and Harvest Moon.

<p>Results represent the mean ± SD of three biological replicates. Minor fatty acids (accounting in total for <3% of the fatty acid composition) are not shown. 16∶0, palmitic acid; 16∶1, palmitoleic acid; 18∶0, stearic acid; 18∶1c9, oleic acid; 18∶1c11, <i>cis</i>-vaccenic acid; 18∶2, linoleic acid; 18∶3, α-linolenic acid.</p

Sea buckthorn sequences associated with isoprenoid biosynthesis by A) cytosolic MVA pathway and B) plastidial MEP pathway.

<p>Enzymes for which sea buckthorn sequences have been identified are shown in green. Abbreviations used for intermediates are: 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA); mevalonic acid (MVA); mevalonate-5-phosphate (MVAP); MVAPP mevalonate-5-diphosphate (MVAPP); Isopentenyl diphosphate (IPP); Dimethylallyl-diphosphate (DMAPP); d-glyceraldehyde-3-phosphate (GAP); 1-deoxy-D-xylulose-5-phosphate (DXP); 2- C-methyl-D- erythritol-4- phosphate (MEP); 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (CDP-ME); 2-phospho-4-(cytidine 5'C-diphospho) 2-C-methyl-D-erythritol (CDP-ME2P); 2-C- methyl-D- erythritol 2,4-cyclodiphosphate (MECDP); 1-hydroxy-3-methyl-2-(E)-butenyl-4-diphosphate (HMBPP); Abbreviations used for enzymes are: Acetoacetyl-CoA thiolase (AACT); 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS); HMG-CoA reductase (HMGR); mevalonate kinase (MVK); diphosphomevalonate kinase (PMK); diphosphomevalonate decarboxylase (PMD); isopentenyl diphosphate isomerase (IPP/IPI); 1-deoxy-d-xylulose-5-phosphate synthase (DXS); 1-deoxy-d-xylulose-5-phosphate reductoisomerase (DXR); 4-diphosphocytidyl-2-C-methyl-d-erythritol synthase (ISPD/CMS); 4-(cytidine 5′-diphospho-2-C-methyl-d-erythritol kinase (ISPE/CMK); 2-C-methyl-d-erythritol 2,4-cyclodiphosphate synthase (ISPF/MCS); 4-hydroxy-3-methyl but-2-enyl diphosphate synthase (ISPG/HDS); 4-hydroxy-3-methyl but-2-enyl diphosphate reductase (ISPH/HDR).</p

Size distribution of sea buckthorn 454 reads and assembled contigs.

<p>Size distribution of sea buckthorn 454 reads and assembled contigs.</p

Sea buckthorn sequences associated with fatty acid A) and triacylglycerol B) biosynthetic pathways.

<p>Enzymes with names in full are represented on the left, and with names in short are represented on the right. Enzymes known to function at specific steps of the pathway are represented in black. Enzymes for which sea buckthorn sequences have been identified are shown in green. EAR, enoyl-acyl-ACP-reductase; HAD, 3-hydroxyacyl-ACP dehydratase. Enzymes responsible for phosphatidylcholine acyl editing in TAG assembly (LPCAT, CPT/PDCT, PDAT) are shown in the shaded box in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034099#pone-0034099-g006" target="_blank">figure 6B</a>.</p

Full-length genes related to abiotic stress response in sea buckthorn seed transcriptome, along with nucleotide identities between Arabidopsis genes and sea buckthorn sequences.

<p>Full-length genes related to abiotic stress response in sea buckthorn seed transcriptome, along with nucleotide identities between Arabidopsis genes and sea buckthorn sequences.</p

Species distribution of top BLAST hits of sea buckthorn sequences with other plant species.

<p>Species distribution of top BLAST hits of sea buckthorn sequences with other plant species.</p

Comparison of ESTs corresponding to fatty acid biosynthesis genes from sea buckthorn, flax and Arabidopsis.

<p>Comparison of ESTs corresponding to fatty acid biosynthesis genes from sea buckthorn, flax and Arabidopsis.</p

RT-PCR analysis of genes involved in fatty acid and triacylglycerol biosynthesis in seed and pulp tissues at different developmental stages of fruit from RC-4 cultivar.

<p>G, G/Y, Y/O and O/R represent fruits harvested at green (6 August), green/yellow (17 August), yellow/orange (31 August) and orange/red (22 October) stages, respectively. <i>Ubiquitin5</i> (<i>UBQ5)</i> was used as loading control.</p

Gene Ontology annotation of sea buckthorn unigenes.

<p>A) Biological process; B) Cellular component; C) Molecular function.</p

List of enzymes and the corresponding Arabidopsis genes involved in fatty acid biosynthesis along with number of sea buckthorn ESTs, and nucleotide identities between Arabidopsis genes and closest sea buckthorn sequences.

<p>List of enzymes and the corresponding Arabidopsis genes involved in fatty acid biosynthesis along with number of sea buckthorn ESTs, and nucleotide identities between Arabidopsis genes and closest sea buckthorn sequences.</p