Analysis of free analyte fractions by rapid affinity chromatography

The invention is generally directed toward an analytical method to determine the concentration of the free analyte fraction in a sample. More particularly, the method encompasses applying a sample comprising a free and bound analyte fraction to an affinity column capable of selectively extracting the free fraction in the millisecond time domain. The signal generated by the free fraction is then quantified by standard analytical detection techniques. The concentration of the free fraction may then be determined by comparison of its signal with that of a calibration curve depicting the signal of known concentration of the same analyte

Aircraft electromagnetic compatibility

Illustrated are aircraft architecture, electromagnetic interference environments, electromagnetic compatibility protection techniques, program specifications, tasks, and verification and validation procedures. The environment of 400 Hz power, electrical transients, and radio frequency fields are portrayed and related to thresholds of avionics electronics. Five layers of protection for avionics are defined. Recognition is given to some present day electromagnetic compatibility weaknesses and issues which serve to reemphasize the importance of EMC verification of equipment and parts, and their ultimate EMC validation on the aircraft. Proven standards of grounding, bonding, shielding, wiring, and packaging are laid out to help provide a foundation for a comprehensive approach to successful future aircraft design and an understanding of cost effective EMC in an aircraft setting

Combining pot, atom and step economy (PASE) in organic synthesis. Synthesis of tetrahydropyran-4-ones

The combination of pot, atom and step economy (PASE) in the synthesis of organic molecules of medium complexity can lead to a significant 'greening' of a synthetic route. This is demonstrated by the synthesis of highly substituted tetrahydropyran-4-ones and is quantified by a series of recognised metrics, which demonstrate the efficiency of combining PASE over conventional synthetic strategies

The Impact of Cell Phones and BAC Laws on Motor Vehicle Fatality Rates

This paper develops a set of models for the determinants of automobile fatalities with particular attention devoted to the effects of increased cell phone usage. Cell phones have been associated with both life-taking and life-saving properties. However, prior statistical evaluations of the effects of cell phones have led to fragile results. We develop in this paper econometric models using time series data, allowing for polynomial structures of the regressors. The models are evaluated with a set of specification error tests providing reliable estimates of the effects of the various policy and driving related variables evaluated. The statistical results indicate the effect of cell phones is non-monotonic depending on the volume of phones in use, first having a net life-taking effect, then a net life-saving effect, followed finally with a net life-taking effect as the volume of phone use increases.Motor Vehicle Fatalities, Cell Phones, BAC Laws

Analysis of free analyte fractions by rapid affinity chromatography

The invention is generally directed toward an analytical method to determine the concentration of the free analyte fraction in a sample. More particularly, the method encompasses applying a sample comprising a free and bound analyte fraction to an affinity column capable of selectively extracting the free fraction in the millisecond time domain. The signal generated by the free fraction is then quantified by standard analytical detection techniques. The concentration of the free fraction may then be determined by comparison of its signal with that of a calibration curve depicting the signal of known concentration of the same analyte

Loading Microcolumns for the Separation of Analytes from a Sample in the Millisecond Time Scale

The present invention generally relates to a microcolumn capable of separating an analyte from a sample in the millisecond time domain. The microcolumn is capable of such rapid separation by employing small column volumes that can tolerate medium to high flow rates. The invention also relates to a method of loading a microcolumn capable of separating an analyte from a sample in the millisecond time domain using plural injections of the packing material

A new adaptive colorization filter for video decompression

HD content is more in demand and requires a lot of bandwidth. In this paper, a new real-time adaptive colorization filter for HD videos is presented. This approach reduces the required bandwidth by reducing non-key frames in the HD video sequence to grayscale and colourizing these frames at the decompression stage. Additionally this technique determines the frame status based on the image information

Characterization of Glycation Adducts On Human Serum Albumin by Matrix Assisted Laser Desorption/ Ionization Time-Of-Flight Mass Spectrometry

Background—Non-enzymatic glycation of human serum albumin (HSA) is associated with the long-term complications of diabetes. We examined the structure and location of modifications on minimally glycated HSA and considered their possible impact on the binding of drugs to this protein. Methods—Minimally glycated and normal HSA (used as a control) were digested with trypsin, Glu-C or Lys-C, followed by fractionation of the resulting peptides and their analysis by matrixassisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) to determine the structures and locations of glycation adducts. Results—Several specific lysine and arginine residues were identified as modification sites in minimally glycated HSA. Residues K12, K51, K199, K205, K439 and K538 were found to be modified through the formation of fructosyl-lysine, while the modification of K159 and K286 involved the formation of pyrraline, Nε-carboxymethyl-lysine respectively. Lysine K378 was found to give Nε-carboxyethyl-lysine in some forms of glycated HSA but fructosyl-lysine in other forms. Residues R160 and R472 produced a modification based on Nε-(5-hydro-4-imidazolon-2-yl) ornithine. Lysine R222 was modified to produce argpyrimidine, Nε-[5-(2,3,4-trihydroxybutyl)-5- hydro-4-imidazolon-2-yl]ornithine or tetrahydropyrimidine. Conclusions—With the exception of K12, K199, K378, K439 and K525, all of the observed sites of modification for minimally glycated HSA were new to this current study. The fact that many of these glycation-related modifications are located at or near known drug binding sites on HSA explains why some differences have been previously noted in the binding of certain drugs to normal vs glycated HSA