Limits on the Peculiar Velocities of Two Distant Clusters Using the Kinematic Sunyaev-Zel'dovich Effect

We report millimeter-wavelength observations of the Sunyaev-Zel'dovich (S-Z) effect in two distant galaxy clusters. A relativistically correct analysis of the S-Z data is combined with the results of X-ray observations to determine the radial peculiar velocities v_r of the clusters. We observed Abell 2163 (z=.201) in three mm-wavelength bands centered at 2.1, 1.4, and 1.1 mm. We report a significant detection of the thermal component of the S-Z effect seen as both a decrement in the brightness of the CMB at 2.1 mm, and as an increment at 1.1 mm. Including uncertainties due to the calibration of the instrument, distribution and temperature of the IC gas, and astrophysical confusion, a simultaneous fit to the data in all three bands gives v_r=+490 +1370/-880 km/s at 68% confidence. We observed Abell 1689 (z=.181) in the 2.1 and 1.4 mm bands. Including the same detailed accounting of uncertainty, a simultaneous fit to the data in both bands gives v_r=+170 +815/-630 km/s. The limits on the peculiar velocities of A2163 and A1689 correspond to deviations from the uniform Hubble flow of <= 2-3%.Comment: 21 pages, 13 postscript figures, LaTeX(aaspptwo.sty), ApJ(in press

Digital deception: Cyber fraud and online misinformation

PHISHING, USER ACCOUNT takeovers, and other computing-related threats have made it easy for criminals to deceive people for financial and other gain. It is now considered standard practice for an advanced cyberattack, even a highly technical one, to start in a nontechnical manner: a spearphishing email deceiving an organization’s employees into providing their credentials, a watering hole website infecting their computer, and so on. It is the human that is the initial target, as well as the first line of defense. At the same time, social media has become a dominant, direct, and highly effective form of news generation and sharing at a global scale, in a manner that influences and enhances, but also challenges and often antagonizes, traditional media corporations

T cell sensitivity to TGF-β is required for the effector function but not the generation of splenic CD8+ regulatory T cells induced via the injection of antigen into the anterior chamber

The introduction of antigen into the anterior chamber (AC) of the eye induces the production of antigen-specific splenic CD8+ regulatory T cells (AC-SPL cells) that suppress a delayed-type hypersensitivity (DTH) reaction in immunized mice. Because the generation of these regulatory T cells is also induced by exposure to transforming growth factor (TGF)-β and antigen or F4/80+ cells exposed to TGF-β and antigen in vitro, we investigated (i) whether these cells are produced in dominant negative receptor for transforming growth factor β receptor type II (dnTGFβRII) or Cbl-b−/− mice whose T cells are resistant to TGF-β, (ii) whether DTH is suppressed by wild type (WT) CD8+ AC-SPL cells in Cbl-b−/− and dnTGFβRII mice and (iii) the effect of antibodies to TGF-β on the suppression of DTH by CD8+ AC-SPL cells. DnTGFβRII immunized and Cbl-b−/− mice produced splenic CD8+ regulatory cells after the intracameral injection of antigen and immunization. The suppression of a DTH reaction by CD8+ AC-SPL cells in WT mice was blocked by the local inclusion of antibodies to TGF-β when WT splenic CD8+ AC-SPL cells were injected into the DTH reaction site. Moreover, the DTH reaction in immunized dnTGFβRII and Cbl-b−/− mice was not suppressed by the transfer of WT CD8+ AC-SPL cells to the site challenged with antigen. In aggregate, these observations suggest that T cell sensitivity to TGF-β is not an obligate requirement for the in vivo induction of CD8+ AC-SPL T cells but the suppression of an in vivo DTH reaction by CD8+ AC-SPL cells is dependent on TGF-β

The Sunyaev-Zel'dovich Infrared Experiment: A Millimeter-wave Receiver for Cluster Cosmology

Measurements of the Sunyaev-Zel'dovich (S-Z) effect towards distant clusters of galaxies can be used to determine the Hubble constant and the radial component of cluster peculiar velocities. Determination of the cluster peculiar velocity requires the separation of the two components of the S-Z effect, which are due to the thermal and bulk velocities of the intracluster plasma. The two components can be separated practically only at millimeter (mm) wavelengths. Measurements of the S-Z effect at mm wavelengths are subject to minimal astrophysical confusion and, therefore, provide an important test of results obtained at longer wavelengths. We describe the instrument used to make the first significant detections of the S-Z effect at millimeter wavelengths. This instrument employs new filter, detector, and readout technologies to produce sensitive measurements of differential sky brightness stable on long time scales. These advances allow drift scan observations which achieve high sensitivity while minimizing common sources of systematic error.Comment: 19 pages, 15 postscript figures, LaTeX(aaspptwo.sty), ApJ(in press

An iron-oxygen intermediate formed during the catalytic cycle of cysteine dioxygenase

Cysteine dioxygenase is a key enzyme in the breakdown of cysteine, but its mechanism remains controversial. A combination of spectroscopic and computational studies provides the first evidence of a short-lived intermediate in the catalytic cycle. The intermediate decays within 20 ms and has absorption maxima at 500 and 640 nm

Shape-based peak identification for ChIP-Seq

We present a new algorithm for the identification of bound regions from ChIP-seq experiments. Our method for identifying statistically significant peaks from read coverage is inspired by the notion of persistence in topological data analysis and provides a non-parametric approach that is robust to noise in experiments. Specifically, our method reduces the peak calling problem to the study of tree-based statistics derived from the data. We demonstrate the accuracy of our method on existing datasets, and we show that it can discover previously missed regions and can more clearly discriminate between multiple binding events. The software T-PIC (Tree shape Peak Identification for ChIP-Seq) is available at http://math.berkeley.edu/~vhower/tpic.htmlComment: 12 pages, 6 figure

Geocentrism reexamined

The universe is nearly isotropic on very large scales. It is much more difficult to show that the universe is radially homogeneous (independent of distance), or equivalently, that it is isotropic about distant points. This taken as an axiom, since if it were not true, then we would occupy a preferred position. This paper considers several empirical arguments for radial homogeneity based on the cosmic microwave background (CMB). The tightest limits on inhomogeneity on the scale of the horizon are of order ten percent but will improve soon. These limits involve the Sunyaev-Zel'dovich effect in clusters of galaxies, excitation of low-energy atomic transitions, and the accurately thermal spectrum of the CMB. Weaker limits from primordial nucleosynthesis are discussed briefly.Comment: RevTeX source, 14 pages, no figs. To appear Phys Rev

Virtual patients design and its effect on clinical reasoning and student experience : a protocol for a randomised factorial multi-centre study

Background Virtual Patients (VPs) are web-based representations of realistic clinical cases. They are proposed as being an optimal method for teaching clinical reasoning skills. International standards exist which define precisely what constitutes a VP. There are multiple design possibilities for VPs, however there is little formal evidence to support individual design features. The purpose of this trial is to explore the effect of two different potentially important design features on clinical reasoning skills and the student experience. These are the branching case pathways (present or absent) and structured clinical reasoning feedback (present or absent). Methods/Design This is a multi-centre randomised 2x2 factorial design study evaluating two independent variables of VP design, branching (present or absent), and structured clinical reasoning feedback (present or absent).The study will be carried out in medical student volunteers in one year group from three university medical schools in the United Kingdom, Warwick, Keele and Birmingham. There are four core musculoskeletal topics. Each case can be designed in four different ways, equating to 16 VPs required for the research. Students will be randomised to four groups, completing the four VP topics in the same order, but with each group exposed to a different VP design sequentially. All students will be exposed to the four designs. Primary outcomes are performance for each case design in a standardized fifteen item clinical reasoning assessment, integrated into each VP, which is identical for each topic. Additionally a 15-item self-reported evaluation is completed for each VP, based on a widely used EViP tool. Student patterns of use of the VPs will be recorded. In one centre, formative clinical and examination performance will be recorded, along with a self reported pre and post-intervention reasoning score, the DTI. Our power calculations indicate a sample size of 112 is required for both primary outcomes

Understanding Climatic Impacts, Vulnerabilities, and Adaptation in the United States: Building a Capacity for Assessment

Based on the experience of the U.S. National Assessment, we propose a program of research and analysis to advance capability for assessment of climate impacts, vulnerabilities, and adaptation options. We identify specific priorities for scientific research on the responses of ecological and socioeconomic systems to climate and other stresses; for improvement in the climatic inputs to impact assessments; and for further development of assessment methods to improve their practical utility to decision-makers. Finally, we propose a new institutional model for assessment, based principally on regional efforts that integrate observations, research, data, applications, and assessment on climate and linked environmental-change issues. The proposed program will require effective collaboration between scientists, resource managers, and other stakeholders, all of whose expertise is needed to define and prioritize key regional issues, characterize relevant uncertainties, and assess potential responses. While both scientifically and organizationally challenging, such an integrated program holds the best promise of advancing our capacity to manage resources and the economy adaptively under a changing climate