Antioxidant and Antiproliferative Potential of Acacia auriculiformis Methanol Leaves Extract Against Breast Cancer Cell Model

Acacia auriculiformis is a plant which often found in Indonesia. Acacia leaves have been known to have high antioxidant content. The phenolic compound of A. auriculiformis is the largest of these plants and phenolic compound have potential as anticancer protective agents. The aims of this study were to determine the phenolic compound, antioxidant activity, cytotoxic and antiproliferative activity of acacia leaves extract against T47D and MCF-7 breast cancer cell lines. Extraction was carried out by maceration method using methanol as a solvent. Phenolic compounds were examined by Thin Layer Chromatography (TLC) and antioxidant activity was measured using DPPH assay, followed by cytotoxicity and antiproliferation test measured by MTT Assay to obtain the IC50 value and percent of antiproliferation. Based on TLC, A. auriculiformis methanol leaves extract contain a phenolic compound with Rf value of 0.85 compared to gallic acid. àAntioxidant activity A. auriculiformis with IC50 value of 9 ppm is classified as high antioxidant activity and MTT assay results for cytotoxicity with IC50 value of 273.8 and 31.26 ppm for T47D and MCF-7 cells, respectively. Antiproliferative analysis also showed high values for MCF-7 cells. These data have shown that methanol leaves extract of A. auriculiformis has high antioxidant activity and inhibited MCF-7 cell proliferation but not T47D breast cancer cell lines.ÃÂ

Effect of Staurosporine on the Intracellular Localization of Hepatitis B Virus Core Protein

protein is also including in the HBV genome targeting into the nucleus through modulating carboxyl residues byphosphorylation. Nuclear localication Signal (NLS) in HBV core protein is inside the virion structure and it must beunmasked in order to function, perhaps by phosphorylation. Phosphorylation of of HBV core protein in turn couldbegin to alter capsid conformation. Staurosporine is a natural product originally isolated from bacteriumStreptomyces staurosporeus. Staurosporine was discovered to have biological activities ranging from anti-fungal toanti-hypertensive. The interest in these activities resulted in a large investigative effort in chemistry and biology andthe discovery of the potential for anti-cancer treatment. The main biological activity of Staurosporine is the inhibitionof protein kinases through the prevention of ATP binding to the kinase. In the present study, we have studied theintracellular localization of EGFP-Core fusion protein with triple HBV core and SV-40 nuclear localization signal atits carboxyl terminal in presence and absence of Staurosporine. We also to study the effect of Staurosporine treatmenton the intracellular localization of EGFP-Core fusion protein in the hepatocyte cells line of HepG2 cell. Resultsshowed that effect of Staurosporine is prevent the nuclear localization of EGFP-Core fusion protein into nucleusthrough an inhibition of the phosphorylation of core protein. Stauroporine also prevents cell division so that passivetrapping of core protein is inhibited

Effect of Nuclear Export Inhibitor Leptomycin Bon the Intracellular Localization of HBV Core Protein into Hepatocytes Cell Line Huh-7 and HepG2 Cells

Leptomycin B (LMB) was originally discovered as a potent anti-fungal antibiotic from Streptomyces species. The cellular target of LMBhas been identified as the nuclear export receptor CRM-1 or exportin-1, which is involved in nuclear trafficking of cellular RNAs or proteins containing the nuclear export sequence (NES). CRM-1 is the main mediator of nuclear export in many cell types including hepatocyte cell lines. The ability of LMB to inhibit nuclear export has made it a useful tool in the study of the intracellular localization of many regulatory proteins. In this study, we evaluated the effect of nuclear export inhibitor LMB treatment on the intracellular localization of HBV core protein into the hepatocyte cell lines, Huh-7 and HepG2 cells. We also reported the quantification of the distribution of EGFP-Core fusion protein with redundant core NLS as well as SV-40 NLS into cell compartments. Results shown that in Huh-7 cells treatment of LMBcaused retention of EGFP-Core fusion protein into the nucleus, so increased the nuclear localization of EGFP-Core and all variants. In HepG2 cells, although not significantly, treatment of LMB increased a number of nuclear localization in all EGFP-Core constructions, even the nuclear localization in HepG2 cells is not so high as in Huh-7 cells

Effect of Nuclear Export Inhibitor Leptomycin B on the Intracellular Localization of HBV Core Protein into Hepatocytes Cell Line Huh-7 and HepG2 Cells

Leptomycin B (LMB) was originally discovered as a potent anti-fungal antibiotic from Streptomyces species. The cellular target of LMB has been identified as the nuclear export receptor CRM-1 or exportin-1, which is involved in nuclear trafficking of cellular RNAs or proteins containing the nuclear export sequence (NES). CRM-1 is the main mediator of nuclear export in many cell types including hepatocyte cell lines. The ability of LMB to inhibit nuclear export has made it a useful tool in the study of the intracellular localization of manyregulatory proteins. In this study, we evaluated the effect of nuclear export inhibitor LMB treatment on the intracellular localization of HBV core protein into the hepatocyte cell lines, Huh-7 and HepG2 cells. We also reported the quantification of the distribution of EGFP-Core fusion protein with redundant core NLS as well as SV-40 NLS into cell compartments. Results shown that in Huh-7 cells treatment of LMB caused retention of EGFP-Core fusion protein into the nucleus, so increased the nuclear localization of EGFP-Core and all variants.In HepG2 cells, although not significantly, treatment of LMB increased a number of nuclear localization in all EGFP-Core constructions, even the nuclear localization in HepG2 cells is not so high as in Huh-7 cells. Keywords: Leptomycin B, HBV, core protein, intracellular localization, NLS, Huh-7, HepG2 cel

In vitro expression of the recombinant fusion protein of Newcastle disease virus from local Indonesian isolates by using a cell-free protein expression system

The aim of this work was the in vitro expression of the recombinant fusion (F) protein of Newcastle disease virus (NDV).  The pBT7-N-His-Fusion-NDV expression plasmid which carries the recombinant F protein encoding gene from local Indonesian isolates, was prepared and transformed into E. coli BL21 (DE3). To detect bacterial colonies carrying the recombinant plasmid, a restriction endonuclease analysis was performed using the EcoRI restriction endonuclease. These results showed that the pBT-N-His-Fusion-NDV plasmid was successfully isolated with a size of 4.601 bp, and three recombinant plasmids carrying the gene coding for the recombinant F protein of NDV were obtained. Selected recombinant plasmids were then in vitro by using a cell-free protein expression system followed by visualization of the recombinant F protein on a 12% SDS-PAGE gel both by Coomassie Brilliant Blue staining and Western blotting. Recombinant F protein was successfully in vitro expressed by using a cell-free protein expression system as indicated by a specific single protein band with a molecular mass of 25.6 kDa

The Effect of Ethanolic Extract of Cashew Fruit Peel on The Liver Histological Structure in Rat (Rattus norvegicus Berkenhout, 1769)

Cashew fruit peel is a waste produced from the cashew nut industry, and it has not been utilized optimally yet. Cashew peel extract has the potential to be used as a contraceptive agent, which capable of reducing reproductive capacity. However, its side effects on other tissue and organ such as liver not clearly studied yet. This study aims to determine the effect of ethanolic extracts of cashew peel on the histological structure of the white rat liver. In this study, 21 female white rats were used and be grouped for control (6 mice) which were treated with CMCMa 0.5% and 15 mice were treated with peel extract of 500 mg/kg body every day for one month. Liver for examination was collected sequentially at 3rd, 5th, 8th, 11th, and 14th of the estrous cycle. The liver was processed for histological observation and stained with Hematoxylin Eosin and Mallory Acid Fuchsin staining solution. The liver hepatocyte was observed for it abnormality and be scored to calculate the number of cell damage or abnormality. The result showed that peel extract-treated mouse liver was similar to control ones; we did not witness any evidence of fibrosis, pyknosis and cellular necrosis on either control or treated mouse. Statistical analysis by SPSS showed that the p-value between the control and treatment groups was 0.078 (> 0.05) so there was no significant difference between control and treatment. It could be concluded that ethanolic extracts of cashew nuts peel with a concentration of 500 mg/kg body weight caused no effect on the mouse liver histological structure. application with reduced-dosages of NPK fertilizers were arranged in a random block design with three replicates. The results show that large quantities of silica bodies attached to the surface of EFB fibers and amounting to 0.44% soluble Si. The FFB data indicated that the application of 75% NPK + 500 kg composted EFB + 2 L BioSilAc/ha/year on a five-year-old plant resulted in higher yield than that obtained from 100% standard dosage of NPK. The study also revealed that the application of EFB compost reduced 50% of BioSilAc dosage

Genotipe Single Nucleotide Polimorphism (SNP) Gen PLA2G10 T512C dan T-123/IN1C pada Penderita Angina Pektoris di Pusat Jantung Nasional Harapan Kita Jakarta, Indonesia

Angina pektoris merupakan salah satu gejala awal yang menjadi penanda masalah kardiovaskuler berat seperti penyakit jantung koroner. Sementara gen PLA2G10 dipercaya memiliki peran, baik dalam progresi aterosklerotik yang menyebabkan deposit plak dan penyumbatan pada arteri koroner, atau justru sebaliknya yaitu antiaterogenik. Polimorfisme PLA2G10 pada titik SNP T512C (rs36072688) dan T-123/in1C (rs4003232) mampu mengubah level ekspresi gen PLA2G10 sebagai gen pengode enzim sPLA2-GX. Penelitian ini bertujuan untuk mengetahui genotip dari PLA2G10 pada titik SNP T512C (rs36072688) and T-123/in1C (rs4003232) para penderita angina pektoris dan mengkaji apakah penyakit disebabkan karena mutasi. Sampel penelitian berupa peripheral blood mononuclear cell koleksi Divisi Litbang Rumah Sakit Jantung dan Pembuluh Darah Harapan Kita. Sampel berjumlah 113, berasal dari penderita angina pektoris dengan dan tanpa plak pada arteri koroner. Hasil penelitian menunjukkan bahwa gen PLA2G10 pada titik SNP T512C (rs36072688) memiliki genotip homozigot wildtype (TT) dan T-123/In1C (rs4003232) memiliki genotip heterozigot (TC) melalui metode TaqMan® SNP Genotyping Assay, dan tidak ada polimorfisme yang ditemukan pada kedua grup sampel tersebut

Pengaruh Pemberian Bubuk Kakao (Theobroma cacao L) Fermentasi Terhadap Profil Lipid Tikus Putih (Rattus norvegicus Berkenhout, 1769) Hiperlipidemia

Kakao (Theobroma cacao L) merupakan salah satu komoditas perkebunan yang besar di Indonesia. Penelitian-penelitian yang telah dilakukan menunjukan bahwa kakao (Theobroma cacao L) mengandung senyawa polifenol antara lain katekin, epikatekin, proantosianidin, dan antosianin yang berpotensi sebagai senyawa antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian bubuk kakao (Theobroma cacao L) fermentasi terhadap profil lipid tikus hiperlipidemia. Penelitian menggunakan Rancangan Acak Lengkap dengan lima perlakuan dan tiga ulangan. Perlakuan terdiri dari kelompok normal, kontrol positif, 3 kelompok uji bubuk kakao fermentasi masing-masing dengan dosis 0,2, 0,4, dan 0,8 gram /200 gram BB. Tikus diberi diet tinggi lemak selama 8 minggu, dan larutan bubuk kakao diberikan 4 minggu terakhir masa penelitian. Pengukuran uji profil lipid darah dilakukan pada minggu ke-0, 4 dan 8. Hasil penelitian menunjukan bahwa pemberian bubuk kakao fermentasi secara oral mampu menurunkan kadar kolesterol total, trigliserida, LDL, serta meningkatkan kadar HDL

Potensi Antioksidan pada Bubuk Kakao (Theobroma cacao L) Fermentasi Dan Non Fermentasi terhadap Kadar Malondialdehid (MDA) Tikus Putih (Rattus norvegicus Berkenhout, 1769) Hiperlipidemia

Indonesia merupakan negara penghasil kakao terbesar ketiga di dunia. Penelitian-penelitian yang telah dilakukan menunjukan bahwa kakao (Theobroma cacao L) mengandung senyawa polifenol antara lain katekin, epikatekin, proantosianidin, dan antosianin yang berpotensi sebagai senyawa antioksidan. Penelitian ini bertujuan untuk mempelajari potensi antioksidan bubuk kakao (Theobroma cacao L) fermentasi dan non fermentasi terhadap kadar malondialdehid (MDA) tikus putih. Penelitian menggunakan Rancangan Acak Lengkap faktorial dengan delapan perlakuan dan tiga ulangan. Perlakuan terdiri dari kelompok normal, kontrol positif, 3 kelompok uji bubuk kakao fermentasi dan 3 kelompok uji bubuk kakao non fermentasi masing-masing dengan dosis 0,2, 0,4, dan 0,8 gram /200 gram BB. Tikus diberi diet tinggi lemak selama 8 minggu, dan larutan bubuk kakao diberikan 4 minggu terakhir masa penelitian. Pengukuran kadar Malondialdehid (MDA) diukur pada minggu ke-4 dan 8 masa penelitian. Hasil penelitian menunjukan bahwa kandungan total fenol bubuk kakao fermentasi lebih besar dari pada bubuk kakao non fermentasi. Pemberian bubuk kakao fermentasi secara oral mampu menurunkan kadar MDA, sedangkan bubuk kakao non fermentasi belum dapat menurunkan kadar MDA

TESTOSTERONE AND CORTISOL LEVEL IN FECAL JAVA DEER (Cervus russa timorensis Mul. & Schl 1844) MALE IN CAPTIVITY BUNDER GUNUNG KIDUL YOGYAKARTA

Common reasons for monitoring hormone concentrations in domestic or wild animals, include breeding management, pregnancy diagnosis, stress assessment, and to diagnosed of endocrine illness. The research goal was to develop non invasive analytical techniques of hormone in Java deer (Cervus russa timorensis Mul. & Schl 1844). The non-invasive methods involve the collection of feces, urine, or even saliva in order to measure the metabolites hormones of interest. Samples obtained from either method are most often analyzed with radioimmunoassay (RIA) or enzyme immunoassay (EIA) technology. Both collection and assay methods have advantages and disadvantages. Choice of method depends on the tractability of the species or individual animal in question and the type of question that the data is expected to answer. We are researching basic questions regarding reproductive endocrinology of male Cervus russa timorensis Mul. & Schl 1844. Understanding C timorensis reproductive physiology will help us better address reproductive challenges in both captive and free-ranging populations. Fecal testosterone and cortisol concentrations were measured in captive male C timorensis. As much as five adult male deer were separated into separate cages. Fecal samples were collected in the morning and evening for 30 days. Sample processed through lyophilization stage, pulverization, solvent extraction with methanol and centrifugation to obtain the supernatant for EIA analysis. EIA test results indicated that content of both testosterone and cortisol could be detected, although the levels were still low. This data show that there is a metabolite in-activism in the fecal. With these results through fecal hormone measurement method (non-invasive) it is possible to be developed for observation and research on the reproductive status of the deer.Keywords: Non-invasive, Testosterone, Cortisol, Java deer