Demosponge EST Sequencing Reveals a Complex Genetic Toolkit of the Simplest Metazoans

Sponges (Porifera) are among the simplest living and the earliest branching metazoans. They hold a pivotal role for studying genome evolution of the entire metazoan branch, both as an outgroup to Eumetazoa and as the closest branching phylum to the common ancestor of all multicellular animals (Urmetazoa). In order to assess the transcription inventory of sponges, we sequenced expressed sequence tag libraries of two demosponge species, Suberites domuncula and Lubomirskia baicalensis, and systematically analyzed the assembled sponge transcripts against their homologs from complete proteomes of six well-characterized metazoans—Nematostella vectensis, Caenorhabditis elegans, Drosophila melanogaster, Strongylocentrotus purpuratus, Ciona intestinalis, and Homo sapiens. We show that even the earliest metazoan species already have strikingly complex genomes in terms of gene content and functional repertoire and that the rich gene repertoire existed even before the emergence of true tissues, therefore further emphasizing the importance of gene loss and spatio-temporal changes in regulation of gene expression in shaping the metazoan genomes. Our findings further indicate that sponge and human genes generally show similarity levels higher than expected from their respective positions in metazoan phylogeny, providing direct evidence for slow rate of evolution in both “basal” and “apical” metazoan genome lineages. We propose that the ancestor of all metazoans had already had an unusually complex genome, thereby shifting the origins of genome complexity from Urbilateria to Urmetazoa

Environmental changes define ecological limits to species richness and reveal the mode of macroevolutionary competition

© 2016 The Authors. Ecology Letters published by CNRS and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. [4.0 License] The attached file is the published version of the articl

Observations of Rocks in Jezero Landing Site: SuperCam/LIBS technique overview of results from the first six months of operations.

On-board the Perseverance rover, the SuperCam instrument is being used as a remote-sensing facility to analyze rocks and soils targets. SuperCam is a suite of five coaligned techniques: just like ChemCam (onboard MSL/Curiosity rover on Mars since 2012), it uses the Laser Induced Breakdown Spectroscopy (LIBS) technique to determine the elementary composition of the targets, but it also uses Raman (for the first time in planetary science) and visible-infrared (VISIR - for the first time in situ) spectroscopic methods in order to access some mineralogical and structural information. A microphone gives access to some physical parameters of the sampled rocks (such as hardness) as well as to some atmospheric parameters (wind direction). These chemical and mineralogical analyses are contextualized thanks to a color remote micro-imager (RMI). In this study, we focus mainly on the LIBS results obtained so far

Dinosaurs reveal the geographical signature of an evolutionary radiation

Dinosaurs dominated terrestrial ecosystems across the globe for over 100 million years and provide a classic example of an evolutionary radiation. However, little is known about how these animals radiated geographically to become globally distributed. Here, we use a biogeographical model to reconstruct the dinosaurs’ ancestral locations, revealing the spatial mechanisms that underpinned this 170-million-year-long radiation. We find that dinosaurs spread rapidly initially, followed by a significant continuous and gradual reduction in their speed of movement towards the Cretaceous/Tertiary boundary (66 million years ago). This suggests that the predominant mode of dinosaur speciation changed through time with speciation originally largely driven by geographical isolation—when dinosaurs speciated more, they moved further. This was gradually replaced by increasing levels of sympatric speciation (species taking advantage of ecological opportunities within their existing environment) as terrestrial space became a limiting factor. Our results uncover the geographical signature of an evolutionary radiation

An insight into ancient aeolian processes and post‐Noachian aqueous alteration in Gale crater, Mars, using ChemCam geochemical data from the Greenheugh capping unit

Aeolian processes have shaped and contributed to the geological record in Gale crater, Mars, long after the fluviolacustrine system existed ∼3 Ga ago. Understanding these aeolian deposits, particularly those which have been lithified and show evidence for aqueous alteration, can help to constrain the environment at their time of deposition and the role of liquid water later in Mars’ history. The NASA Curiosity rover investigated a prominent outcrop of aeolian sandstone within the Stimson formation at the Greenheugh pediment as part of its investigation of the Glen Torridon area. In this study, we use geochemical data from ChemCam to constrain the effects of aeolian sedimentary processes, sediment provenance, and diagenesis of the sandstone at the Greenheugh pediment, comparing the Greenheugh data to the results from previous Stimson localities situated 2.5 km north and >200 m lower in elevation. Our results, supported by mineralogical data from CheMin, show that the Stimson formation at the Greenheugh pediment was likely sourced from an olivine-rich unit that may be present farther up the slopes of Gale crater’s central mound. Our results also suggest that the Greenheugh pediment Stimson formation was cemented by surface water runoff such as that which may have formed Gediz Vallis. The lack of alteration features in the Stimson formation at the Greenheugh pediment relative to those of the Emerson and Naukluft plateaus suggests that groundwater was not as available at this locality compared to the others. However, all sites share diagenesis at the unconformity

Designing an environmental flow framework for impounded river systems through modelling of invertebrate habitat quality

Many rivers have undergone flow modification by impoundments to provide services such as water supply and hydropower. There is an established consensus that typical modified flow regimes do not sufficiently cater to the needs of downstream ecosystems, and more must be done to understand and mitigate their associated impacts. This study presents a novel, transferable framework by which a small-scale impoundment in North West England is assessed through the use of linked hydro-ecological modelling in SRH-2D and CASiMiR, utilising flow velocity measurements and macroinvertebrate sampling data. Model predictions of habitat quality were supplemented by established ecological principles such as the importance of flow heterogeneity. Results are used to design environmental flow regimes, with the aim of improving ecological metrics whilst considering conflicting water demands. Based on an analysis of historical flow records, the implementation of designer flows over a 12 month period demonstrated increased peak species habitat qualities of 23–26%, characteristics such as flow heterogeneity were more naturalised, and 22% less water was released from the impoundment. Should outcomes be validated by in-stream flow experiment, there is great potential for further development and application of this method, including regional transferability for the rapid designation of environmental flows across a number of sites of similar magnitude and geography

Manganese-Iron Phosphate Nodules at the Groken Site, Gale Crater, Mars

The MSL Curiosity rover investigated dark, Mn-P-enriched nodules in shallow lacustrine/fluvial sediments at the Groken site in Glen Torridon, Gale Crater, Mars. Applying all relevant information from the rover, the nodules are interpreted as pseudomorphs after original crystals of vivianite, (Fe2+,Mn2+)3(PO4)2·8H2O, that cemented the sediment soon after deposition. The nodules appear to have flat faces and linear boundaries and stand above the surrounding siltstone. ChemCam LIBS (laser-induced breakdown spectrometry) shows that the nodules have MnO abundances approximately twenty times those of the surrounding siltstone matrix, contain little CaO, and have SiO2 and Al2O3 abundances similar to those of the siltstone. A deconvolution of APXS analyses of nodule-bearing targets, interpreted here as representing the nodules’ non-silicate components, shows high concentrations of MnO, P2O5, and FeO and a molar ratio P/Mn = 2. Visible to near-infrared reflectance of the nodules (by ChemCam passive and Mastcam multispectral) is dark and relatively flat, consistent with a mixture of host siltstone, hematite, and a dark spectrally bland material (like pyrolusite, MnO2). A drill sample at the site is shown to contain minimal nodule material, implying that analyses by the CheMin and SAM instruments do not constrain the nodules’ mineralogy or composition. The fact that the nodules contain P and Mn in a small molar integer ratio, P/Mn = 2, suggests that the nodules contained a stoichiometric Mn-phosphate mineral, in which Fe did (i.e., could) not substitute for Mn. The most likely such minerals are laueite and strunzite, (Fe2+,Mn2+)3(PO4)2·8H2O and –6H2O, respectively, which occur on Earth as alteration products of other Mn-bearing phosphates including vivianite. Vivianite is a common primary and diagenetic precipitate from low-oxygen, P-enriched waters. Calculated phase equilibria show Mn-bearing vivianite could be replaced by laueite or strunzite and then by hematite plus pyrolusite as the system became more oxidizing and acidic. These data suggest that the nodules originated as vivianite, forming as euhedral crystals in the sediment, enclosing sediment grains as they grew. After formation, the nodules were oxidized—first to laueite/strunzite yielding the diagnostic P/Mn ratio, and then to hematite plus an undefined Mn oxy-hydroxide (like pyrolusite). The limited occurrence of these Mn-Fe-P nodules, both in space and time (i.e., stratigraphic position), suggests a local control on their origin. By terrestrial analogies, it is possible that the nodules precipitated near a spring or seep of Mn-rich water, generated during alteration of olivine in the underlying sediments

The Horses of Realism: The Layton-Pacey Correspondence

Early in 1955, Desmond Pacey and Irving Layton met in Montreal, and so began a long friendship and an invaluable correspondence documenting the development of Canadian poetry and criticism after World War II, as well as the development of a major critic and pre-eminent poet. The letters are rich in personal drama, recording the triumphs and setbacks of both men, the tensions in their friendships, their often conflicting views on poetry (especially Canadian poetry) and on the role of the poet and critic in contemporary society. There are discussions of literary figures past and present, heated arguments on current political and social happenings, an exchange of bawdy jokes, and some frank praises and criticisms of each other's work. The tone ranges from jocularity to bitter disappointment, from anger to tenderness, from weariness to excitement