Non-Target Feeding by Galerucella Calmariensis on Sandbar Willow (Salix Interior) in Illinois

Purple loosestrife, Lythrum salicaria L., is an introduced Eurasian perennial herb that has been in North America since the early 1800s (Thompson et al. 1987). Purple loosestrife has been considered a serious threat to the integrity of North American wetlands (Thompson et al. 1987, Blossey et al. 2001a). L. salicaria has been the target of a significant North American project using biological control to combat it (Malecki et al. 1993, Blossey et al. 2001a)

Arthropods Associated With Purple Loosestrife in Illinois Wetlands

We estimated and described the arthropod fauna on purple loosestrife (Lythrum salicaria) plants in northeastern Illinois wetlands. A total of 1063 individual arthropods were collected—930 insects and 133 arachnids. The av- erage number of arthropods ranged from 19.00 to 86.75 individuals per loos- estrife plants of a single root crown. We collected individuals from twenty-five families representing 8 orders of insects. Miridae, Anthocoridae, Lygaeidae, Cicadellidae, and Aphididae were each represented by at least 50 individuals. Three orders and ten families were found that had not been previously reported as occurring on purple loosestrife, but many of these taxa were represented by only a few individuals

The Distribution and Prevalence of the Alfalfa Blotch Leafminer (Diptera: Agromyzidae) in Illinois

Surveys were made in 2001 and 2002 to determine the distribution and prevalence of the alfalfa blotch leafminer, Agromyza frontella (Diptera: Agromyzidae), and its primary parasitoids, Dacnusa dryas and Chrysocharis liriomyzae (Hymenoptera: Braconidae and Eulophidae, respectively), in Illinois. We collected 239 samples from alfalfa fields in the alfalfa-producing regions of Illinois. Samples from each site consisted of 20 cut stems and 10 sets of 10 sweeps in 2001, and 40 stems and 10 sets of 20 sweeps in 2002. Each trifoliate was examined for mining and pinholing injury, and the number of adult A. frontella, D. dryas, and C. liriomyzae per 10 sweeps was calculated for each site. We found that 16.3% of the sites were infested with A. frontella, and all infested fields were north of 39.95 N latitude. Although up to 95% of stems were injured at some sites, mean injury per stem never exceeded 12% of trifoliates. Maximum adult abundance was 8.1 adults per 10 sweeps. We did not detect D. dryas or C. liriomyzae in any of the sweep samples. Because of consistently low populations and the relatively slow rate of A. frontella’s spread in Illinois, we conclude that A. frontella is not a serious threat to alfalfa production in this state at this time

Non-Target Feeding by Galerucella Calmariensis on Sandbar Willow (Salix Interior) in Illinois

Purple loosestrife, Lythrum salicaria L., is an introduced Eurasian perennial herb that has been in North America since the early 1800s (Thompson et al. 1987). Purple loosestrife has been considered a serious threat to the integrity of North American wetlands (Thompson et al. 1987, Blossey et al. 2001a). L. salicaria has been the target of a significant North American project using biological control to combat it (Malecki et al. 1993, Blossey et al. 2001a)

Arthropods Associated With Purple Loosestrife in Illinois Wetlands

We estimated and described the arthropod fauna on purple loosestrife (Lythrum salicaria) plants in northeastern Illinois wetlands. A total of 1063 individual arthropods were collected—930 insects and 133 arachnids. The av- erage number of arthropods ranged from 19.00 to 86.75 individuals per loos- estrife plants of a single root crown. We collected individuals from twenty-five families representing 8 orders of insects. Miridae, Anthocoridae, Lygaeidae, Cicadellidae, and Aphididae were each represented by at least 50 individuals. Three orders and ten families were found that had not been previously reported as occurring on purple loosestrife, but many of these taxa were represented by only a few individuals

The Distribution and Prevalence of the Alfalfa Blotch Leafminer (Diptera: Agromyzidae) in Illinois

Surveys were made in 2001 and 2002 to determine the distribution and prevalence of the alfalfa blotch leafminer, Agromyza frontella (Diptera: Agromyzidae), and its primary parasitoids, Dacnusa dryas and Chrysocharis liriomyzae (Hymenoptera: Braconidae and Eulophidae, respectively), in Illinois. We collected 239 samples from alfalfa fields in the alfalfa-producing regions of Illinois. Samples from each site consisted of 20 cut stems and 10 sets of 10 sweeps in 2001, and 40 stems and 10 sets of 20 sweeps in 2002. Each trifoliate was examined for mining and pinholing injury, and the number of adult A. frontella, D. dryas, and C. liriomyzae per 10 sweeps was calculated for each site. We found that 16.3% of the sites were infested with A. frontella, and all infested fields were north of 39.95 N latitude. Although up to 95% of stems were injured at some sites, mean injury per stem never exceeded 12% of trifoliates. Maximum adult abundance was 8.1 adults per 10 sweeps. We did not detect D. dryas or C. liriomyzae in any of the sweep samples. Because of consistently low populations and the relatively slow rate of A. frontella’s spread in Illinois, we conclude that A. frontella is not a serious threat to alfalfa production in this state at this time

A configuration system for the ATLAS trigger

The ATLAS detector at CERN's Large Hadron Collider will be exposed to proton-proton collisions from beams crossing at 40 MHz that have to be reduced to the few 100 Hz allowed by the storage systems. A three-level trigger system has been designed to achieve this goal. We describe the configuration system under construction for the ATLAS trigger chain. It provides the trigger system with all the parameters required for decision taking and to record its history. The same system configures the event reconstruction, Monte Carlo simulation and data analysis, and provides tools for accessing and manipulating the configuration data in all contexts.Comment: 4 pages, 2 figures, contribution to the Conference on Computing in High Energy and Nuclear Physics (CHEP06), 13.-17. Feb 2006, Mumbai, Indi

Tumour necrosis factor production and natural killer cell activity in peripheral blood during treatment with recombinant tumour necrosis factor.

Tumour necrosis factor (TNF) has been found to be an important immunomodulator. Among other functions TNF activates natural killer (NK) cells and stimulates monocytes/macrophages in an autocrine fashion. TNF production and NK activity in peripheral blood mononuclear cells were determined in a clinical phase I study in which recombinant human (rh) TNF was administered as a continuous infusion weekly for a period of 8 weeks. Even though TNF production and NK activity were significantly reduced directly after rhTNF infusion the effect proved to be transient and most pronounced at the first rhTNF administration. One day after completion of the rhTNF infusion the peripheral cells released more TNF into the supernatant compared to TNF activity immediately before the rhTNF infusion. This effect was conspicuous in non-stimulated cultures. After repeated rhTNF infusions both stimulated and non-stimulated TNF production of the peripheral blood mononuclear cells was increased. NK cell activity was also enhanced after repeated cycles of rhTNF administration as compared to early rhTNF treatment. Thus, repeated rhTNF infusions lead to a stimulatory effect on TNF production and NK activity of peripheral blood cells

A two-domain elevator mechanism for sodium/proton antiport

Sodium/proton (Na+/H+) antiporters, located at the plasma membrane in every cell, are vital for cell homeostasis1. In humans, their dysfunction has been linked to diseases, such as hypertension, heart failure and epilepsy, and they are well-established drug targets2. The best understood model system for Na+/H+ antiport is NhaA from Escherichia coli1, 3, for which both electron microscopy and crystal structures are available4, 5, 6. NhaA is made up of two distinct domains: a core domain and a dimerization domain. In the NhaA crystal structure a cavity is located between the two domains, providing access to the ion-binding site from the inward-facing surface of the protein1, 4. Like many Na+/H+ antiporters, the activity of NhaA is regulated by pH, only becoming active above pH 6.5, at which point a conformational change is thought to occur7. The only reported NhaA crystal structure so far is of the low pH inactivated form4. Here we describe the active-state structure of a Na+/H+ antiporter, NapA from Thermus thermophilus, at 3 Å resolution, solved from crystals grown at pH 7.8. In the NapA structure, the core and dimerization domains are in different positions to those seen in NhaA, and a negatively charged cavity has now opened to the outside. The extracellular cavity allows access to a strictly conserved aspartate residue thought to coordinate ion binding1, 8, 9 directly, a role supported here by molecular dynamics simulations. To alternate access to this ion-binding site, however, requires a surprisingly large rotation of the core domain, some 20° against the dimerization interface. We conclude that despite their fast transport rates of up to 1,500 ions per second3, Na+/H+ antiporters operate by a two-domain rocking bundle model, revealing themes relevant to secondary-active transporters in general