Raphide Crystal Cell Development in Leaves of Psychotria Punctata (Rubiaceae)

The distribution and development of raphide crystal cells in nodulated leaves of Psychotria punctatawere studied by light and electron microscopy. Crystal cells in the leaf are oriented in various ways depending on whether they occur in the spongy or palisade parenchyma. Crystals are never found within the bacterial nodules and are not concentrated around them. Developing leaf crystal cells become larger than surrounding cells and have larger nuclei and nucleoli. Raphides develop within membrane chambers in the large central vacuole in association with membrane complexes, vesicles and tubules, the latter measuring 10-13 nm in diameter. Certain cytoplasmic organelles, the plasmalemma, and a cytoplasmic vacuolar channel complex also appear to be associated with crystal development. These results are compared with other recent investigations dealing with calcium oxalate crystals in higher plants.This article is from Journal of Cell Science 11 (1972): 339. Posted with permission.</p

Method trends and method needs: Examining methods needed for accelerating the field

To define research and statistical methods needed for relevant research and the development of the family business field, this article reviews the past 30 years of analytic and statistical methods used by family business researchers. The article explores the many reasons for studying research methods, especially in family business, and examines the progression and development of methodologies, sample sizes and related methodological issues, as well as theories and topics studied in family business research. Directions for future research highlight methods that we believe should be used to advance family business theory and practice

Method trends and method needs: examining methods needed for accelerating the field

To define research and statistical methods needed for relevant research and the development of the family business field, this article reviews the past 30 years of analytic and statistical methods used by family business researchers. The article explores the many reasons for studying research methods, especially in family business, and examines the progression and development of methodologies, sample sizes and related methodological issues, as well as theories and topics studied in family business research. Directions for future research highlight methods that we believe should be used to advance family business theory and practice