Quantification of three macrolide antibiotics in pharmaceutical lots by HPLC: Development, validation and application to a simultaneous separation

A new validated high performance liquid chromatographic (HPLC) method with rapid analysis time and high efficiency, for the analysis of erythromycin, azithromycin and spiramycin, under isocratic conditions with ODB RP18 as a stationary phase is described. Using an eluent composed of acetonitrile –2-methyl-2-propanol –hydrogenphosphate buffer, pH 6.5, with 1.5% triethylamine (33:7: up to 100, v/v/v), delivered at a flow-rate of 1.0 mL min-1. Ultra Violet (UV) detection is performed at 210 nm. The selectivity is satisfactory enough and no problematic interfering peaks are observed. The procedure is quantitatively characterized and repeatability, linearity, detection and quantification limits are very satisfactory. The method is applied successfully for the assay of the studied drugs in pharmaceutical dosage forms as tablets and powder for oral suspension. Recovery experiments revealed recovery of 97.13–100.28%

Repercussion of anthropogenic landscape changes on pedodiversity and preservation of the pedological heritage

Over a period of time people have lived in and with their surrounding landscapes and for several thousand years transformed the soilscapes and the vegetation into cultural landscape types important for their economy and to meet their needs (Richter 2007, Ellis 2011, Hjelle 2012). The sustainable provision of goods and services depends critically on managing soils without damaging the natural soilscapes and the related natural resources. To support the transition towards sustainable development, science needs to understand how land-use change affects the environment and how this, in turn, feeds back into human livelihood strategies or infl uences the vulnerability of the environment (Rounsevell et al. 2012a). Interactions between decision-making, governance structures, production and consumption, technology, ecosystem services and global environmental change infl uence human activities at the local and regional scale, and are infl uenced by and feed back to the global scale, thereby shaping trajectories of human–environment interaction in land systems (Lambin and Meyfroidt 2011)