The MH1 domain of Smad3 interacts with Pax6 and represses autoregulation of the Pax6 P1 promoter

Pax6 transcription is under the control of two main promoters (P0 and P1), and these are autoregulated by Pax6. Additionally, Pax6 expression is under the control of the TGFβ superfamily, although the precise mechanisms of such regulation are not understood. The effect of TGFβ on Pax6 expression was studied in the FHL124 lens epithelial cell line and was found to cause up to a 50% reduction in Pax6 mRNA levels within 24 h. Analysis of luciferase reporters showed that Pax6 autoregulation of the P1 promoter, and its induction of a synthetic promoter encoding six paired domain-binding sites, were significantly repressed by both an activated TGFβ receptor and TGFβ ligand stimulation. Subsequently, a novel Pax6 binding site in P1 was shown to be necessary for autoregulation, indicating a direct influence of Pax6 protein on P1. In transfected cells, and endogenously in FHL124 cells, Pax6 co-immunoprecipitated with Smad3 following TGFβ receptor activation, while in GST pull-down experiments, the MH1 domain of Smad3 was observed binding the RED sub-domain of the Pax6 paired domain. Finally, in DNA adsorption assays, activated Smad3 inhibited Pax6 from binding the consensus paired domain recognition sequence. We hypothesize that the Pax6 autoregulatory loop is targeted for repression by the TGFβ/Smad pathway, and conclude that this involves diminished paired domain DNA-binding function resulting from a ligand-dependant interaction between Pax6 and Smad3

Three-dimensional distribution of ejecta in Supernova 1987A at 10 000 days

Due to its proximity, SN 1987A offers a unique opportunity to directly observe the geometry of a stellar explosion as it unfolds. Here we present spectral and imaging observations of SN 1987A obtained ~10,000 days after the explosion with HST/STIS and VLT/SINFONI at optical and near-infrared wavelengths. These observations allow us to produce the most detailed 3D map of H-alpha to date, the first 3D maps for [Ca II] \lambda \lambda 7292, 7324, [O I] \lambda \lambda 6300, 6364 and Mg II \lambda \lambda 9218, 9244, as well as new maps for [Si I]+[Fe II] 1.644 \mu m and He I 2.058 \mu m. A comparison with previous observations shows that the [Si I]+[Fe II] flux and morphology have not changed significantly during the past ten years, providing evidence that it is powered by 44Ti. The time-evolution of H-alpha shows that it is predominantly powered by X-rays from the ring, in agreement with previous findings. All lines that have sufficient signal show a similar large-scale 3D structure, with a north-south asymmetry that resembles a broken dipole. This structure correlates with early observations of asymmetries, showing that there is a global asymmetry that extends from the inner core to the outer envelope. On smaller scales, the two brightest lines, H-alpha and [Si I]+[Fe II] 1.644 \mu m, show substructures at the level of ~ 200 - 1000 km/s and clear differences in their 3D geometries. We discuss these results in the context of explosion models and the properties of dust in the ejecta.Comment: Accepted for publication in Ap

SN Ib 1990I: Clumping and Dust in the Ejecta?

Photometry and spectra of the type Ib SN 1990I are presented and analysed, covering about 400 days of evolution. The presence of optical helium lines is shown. SN 1990I seems to show higher velocities compared to a sample of type Ib events. The nebular emission lines display a high degree of asymmetry and the presence of fine structures. Using the [O I] 6300,64A flux, we estimate a lower limit on the oxygen mass to fall in the range (0.7-1.35) M_solar. The oxygen mass requires a filling factor as small as 10^{-2} on day 254, indicating a highly clumpy distribution of the oxygen material. A blueshift of the order 600 km/s is reported in the [O I] 6300,64A after day 254. The [Ca II] 7307.5A emission profile appears blueshifted as well at late epochs . We recover the quasi-bolometric "BVRI" light curve of SN 1990I. The constructed bolometric light curve shows a change of slope at late phases, with an e-folding time of 60 +/-2 d in the [50:200] d time interval, considerably faster than the one of {56}Co decay (i.e. 111.3 d), suggesting the gamma-rays escape with lower deposition, owing to the low mass nature of the ejecta. After day 200, an $e$-folding time ~47 +/-2.8 d is measured. A simplified gamma-ray deposition model is applied after adding a contribution of about 35% to the computed pseudo-bolometric light curves to account for near-IR luminosities to estimate the ejecta and {56}Ni masses (M({56}Ni)=0.11 M_solar and M_{ej}=3.7 M_solar). The deficit in luminosity is estimated to be about 50% around day 308. The observed spectral blueshift combined with the dramatic and sudden drop in the pseudo-bolometric light curve and (B-V) colour is interpreted to be a consequence of dust condensation in the ejecta of SN 1990I around day 250.Comment: 17 pages, 11 gigures and 5 tables. To appear in A & A (accepted

The Axially Symmetric Ejecta of Supernova 1987A

Extensive early observations proved that the ejecta of supernova 1987A (SN 1987A) are aspherical. Fifteen years after the supernova explosion, the Hubble Space Telescope has resolved the rapidly expanding ejecta. The late-time images and spectroscopy provide a geometrical picture that is consistent with early observations and suggests a highly structured, axially symmetric geometry. We present here a new synthesis of the old and new data. We show that the Bochum event, presumably a clump of $^{56}$Ni, and the late-time image, the locus of excitation by $^{44}$Ti, are most naturally accounted for by sharing a common position angle of about 14\degree, the same as the mystery spot and early speckle data on the ejecta, and that they are both oriented along the axis of the inner circumstellar ring at 45\degree to the plane of the sky. We also demonstrate that the polarization represents a prolate geometry with the same position angle and axis as the early speckle data and the late-time image and hence that the geometry has been fixed in time and throughout the ejecta. The Bochum event and the Doppler kinematics of the [Ca II]/[O II] emission in spatially resolved HST spectra of the ejecta can be consistently integrated into this geometry. The radioactive clump is deduced to fall approximately along the axis of the inner circumstellar ring and therefore to be redshifted in the North whereas the [Ca II]/[O II] 7300 \AA emission is redshifted in the South. We present a jet-induced model for the explosion and argue that such a model can account for many of the observed asymmetries. In the jet models, the oxygen and calcium are not expected to be distributed along the jet, but primarily in an expanding torus that shares the plane and northern blue shift of the inner circumstellar ring.Comment: To Appear in Ap

SN 2006oz: rise of a super-luminous supernova observed by the SDSS-II SN Survey

We study SN 2006oz, a newly-recognized member of the class of H-poor, super-luminous supernovae. We present multi-color light curves from the SDSS-II SN Survey, that cover the rise time, as well as an optical spectrum that shows that the explosion occurred at z~0.376. We fitted black body functions to estimate the temperature and radius evolution of the photosphere and used the parametrized code SYNOW to model the spectrum. We constructed a bolometric light curve and compared it with explosion models. The very early light curves show a dip in the g- and r-bands and a possible initial cooling phase in the u-band before rising to maximum light. The bolometric light curve shows a precursor plateau with a duration of 6-10 days in the rest-frame. A lower limit of M_u < -21.5 can be placed on the absolute peak luminosity of the SN, while the rise time is constrained to be at least 29 days. During our observations, the emitting sphere doubled its radius to 2x10^15 cm, while the temperature remained hot at 15000 K. As for other similar SNe, the spectrum is best modeled with elements including O II and Mg II, while we tentatively suggest that Fe III might be present. We suggest that the precursor plateau might be related to a recombination wave in a circumstellar medium (CSM) and discuss whether this is a common property of all similar explosions. The subsequent rise can be equally well described by input from a magnetar or by ejecta-CSM interaction, but the models are not well constrained owing to the lack of post-maximum observations, and CSM interaction has difficulties accounting for the precursor plateau self-consistently. Radioactive decay is less likely to be the mechanism that powers the luminosity. The host galaxy, detected in deep imaging with the 10 m GTC, is a moderately young and star-forming, but not a starburst, galaxy. It has an absolute magnitude of M_g = -16.9.Comment: Contains minor changes (of editorial nature) with respect to v1 in order to match the published version. The abstract has been modified to fit the arXiv space requirements. 11 pages, 8 figures, 3 table

Long duration gamma-ray bursts: hydrodynamic instabilities in collapsar disks

We present 3D numerical simulations of the early evolution of long-duration gamma-ray bursts in the collapsar scenario. Starting from the core-collapse of a realistic progenitor model, we follow the formation and evolution of a central black hole and centrifugally balanced disk. The dense, hot accretion disk produces freely-escaping neutrinos and is hydrodynamically unstable to clumping and to forming non-axisymmetric (m=1, 2) modes. We show that these spiral structures, which form on dynamical timescales, can efficiently transfer angular momentum outward and can drive the high required accretion rates (>=0.1-1 M_sun) for producing a jet. We utilise the smoothed particle hydrodynamics code, Gadget-2, modified to implement relevant microphysics, such as cooling by neutrinos, a plausible treatment approximating the central object and relativistic effects. Finally, we discuss implications of this scenario as a source of energy to produce relativistically beamed gamma-ray jets.Comment: accepted by MNRAS; 32 pages, 46 figure

Ethnic Variation in Inflammatory Profile in Tuberculosis

PMCID: PMC3701709This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Dust and the type II-plateau supernova 2004et

We present mid-infrared (MIR) observations of the Type II-plateau supernova (SN) 2004et, obtained with the {\it Spitzer Space Telescope} between days 64 and 1406 past explosion. Late-time optical spectra are also presented. For the period 300-795 days past explosion, we argue that the spectral energy distribution of SN 2004et comprises (a) a hot component due to emission from optically thick gas, as well as free-bound radiation, (b) a warm component due to newly formed, radioactively heated dust in the ejecta, and (c) a cold component due to an IR echo from the interstellar-medium dust of the host galaxy, NGC 6946. There may also have been a small contribution to the IR SED due to free-free emission from ionised gas in the ejecta. We reveal the first-ever spectroscopic evidence for silicate dust formed in the ejecta of a supernova. This is supported by our detection of a large, but progressively declining, mass of SiO. However, we conclude that the mass of directly detected ejecta dust grew to no more than a few times 10^(-4)Msun. We also provide evidence that the ejecta dust formed in comoving clumps of fixed size. We argue that, after about two years past explosion, the appearance of wide, box-shaped optical line profiles was due to the impact of the ejecta on the progenitor circumstellar medium and that the subsequent formation of a cool, dense shell was responsible for a later rise in the MIR flux. This study demonstrates the rich, multi-faceted ways in which a typical core-collapse supernova and its progenitor can produce and/or interact with dust grains. The work presented here adds to the growing number of studies which do not support the contention that SNe are responsible for the large mass of observed dust in high-redshift galaxies.Comment: (ApJ; in press