Acute sterfte bij het rund: autopsieprotocol en retrsospectieve studie

Sudden death is defined as the unexpected death of an apparently healthy animal within 24 hours after onset of the symptoms. In literature, many causes of sudden death have been reported. In the field, it is very difficult to confirm the exact cause of sudden death only by necropsy. Nevertheless, an exact diagnosis is requested in case of insurance expertise or herd health problems. In the present article, a practical protocol for approaching sudden death in cattle is proposed. The protocol is based on information available in the literature and on a retrospective necropsy series of sudden death cases in cattle in Flanders (n=124). The most common causes were enterotoxemia (23.7%), acute pneumonia (9.3%) and Taxus baccata intoxication (6.8%)

Lightning related fatalities in livestock: veterinary expertise and the added value of lightning location data

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Proteomic studies of a sugar-beet plant resistant to rhizomania

Immuno-Maldi (2001-2004) : aspects fondamentaux et appliqués du développement de biosenseurs (protein arrays, tissue arrays

Field evaluation of willow under short rotation coppice for phytomanagement of metal-polluted agricultural soils

Short rotation coppice (SRC) of willow and poplar might be a promising phytoremediation option since it uses fast growing, high biomass producing tree species with often a sufficient metal uptake. This study evaluates growth, metal uptake and extraction potentials of eight willow clones (Belders, Belgisch Rood, Christina, Inger, Jorr, Loden, Tora and Zwarte Driebast) on a metal-contaminated agricultural soil, with total cadmium (Cd) and zinc (Zn) concentrations of 6.5 +/- 0.8 and 377 +/- 69mg kg1 soil, respectively. Although, during the first cycle, on average generally low productivity levels (3.7 ton DM (dry matter) ha1 y1) were obtained on this sandy soil, certain clones exhibited quite acceptable productivity levels (e.g. Zwarte Driebast 12.5 ton DM ha1 y1). Even at low biomass productivity levels, SRC of willow showed promising removal potentials of 72g Cd and 2.0kg Zn ha1 y1, which is much higher than e.g. energy maize or rapeseed grown on the same soil. Cd and Zn removal can be increased by 40% if leaves are harvested as well. Nevertheless, nowadays the wood price remains the most critical factor in order to implement SRC as an acceptable, economically feasible alternative crop on metal-contaminated agricultural soils

Expression of the Beet necrotic yellow vein virus p25 protein induces hormonal changes and a root branching phenotype in Arabidopsis thaliana

International audienceThe RNA-3-encoded p25 protein was previously characterized as one of the major symptom determinants of the Beet necrotic yellow vein virus. Previous analyses reported the influence of the p25 protein in root proliferation phenotype observed in rhizomania disease on infected sugar beets (Beta vulgaris). A transgenic approach was developed, in which the p25 protein was constitutively expressed in Arabidopsis thaliana Columbia (Col-0) ecotype in order to provide new clues as to how the p25 protein might promote alone disease development and symptom expression. Transgenic plants were characterized by Southern blot and independent lines carrying single and multiple copies of the transgene were selected. Mapping of the T-DNA insertion was performed on the monocopy homozygote lines. P25 protein was localized both in the nucleus and in the cytoplasm of epidermal and root cells of transgenic plants. Although A. thaliana was not described as a susceptible host for BNYVV infection, abnormal root branching was observed on p25 protein-expressing A. thaliana plants. Moreover, these transgenic plants were more susceptible than wild-type plants to auxin analog treatment (2,4-D) but more resistant to methyl jasmonate (MeJA), abscisic acid (ABA) and to lesser extend to salicylic acid (SA). Hormonal content assays measuring plant levels of auxin (IAA), jasmonate (JA) and ethylene precursor (ACC) revealed major hormonal changes. Global transcript profiling analyses on roots displayed differential gene expressions that could corroborate root branching phenotype and stress signaling modifications