Method and apparatus for aligning a laser beam projector Patent

Laser beam projector for continuous, precise alignment between target, laser generator, and astronomical telescope during trackin

Method of directing a laser beam with very high accuracy

System will collimate and direct an argon laser beam with high angular tracking accuracy at objects on the moons surface. It can be adapted to missile and satellite tracking

A standardized database of Last Interglacial (MIS 5e) sea-level indicators in Southeast Asia

Marine Isotope Stage 5e (MIS 5e; the Last Interglacial, 125 ka) represents a process analog for a warmer world. Analysis of sea-level proxies formed in this period helps in constraining both regional and global drivers of sea-level change. In Southeast Asia, several studies have reported elevation and age information on MIS 5e sea-level proxies, such as fossil coral reef terraces or tidal notches, but a standardized database of such data was hitherto missing. In this paper, we produced such a sea-level database using the framework of the World Atlas of Last Interglacial Shorelines (WALIS; https://warmcoasts.eu/world-atlas.html). Overall, we screened and reviewed 14 studies on Last Interglacial sea-level indicators in Southeast Asia, from which we report 43 proxies (42 coral reef terraces and 1 tidal notch) that were correlated to 134 dated samples. Five data points date to MIS 5a (80 ka), six data points are MIS 5c (100 ka), and the rest are dated to MIS 5e. The database compiled in this study is available at 10.5281/zenodo.5040784 (Maxwell et al., 2021)

Proactive and reactive cognitive control and dorsolateral prefrontal cortex dysfunction in first episode schizophrenia.

Cognitive control deficits have been consistently documented in patients with schizophrenia. Recent work in cognitive neuroscience has hypothesized a distinction between two theoretically separable modes of cognitive control-reactive and proactive. However, it remains unclear the extent to which these processes are uniquely associated with dysfunctional neural recruitment in individuals with schizophrenia. This functional magnetic resonance imaging (fMRI) study utilized the color word Stroop task and AX Continuous Performance Task (AX-CPT) to tap reactive and proactive control processes, respectively, in a sample of 54 healthy controls and 43 patients with first episode schizophrenia. Healthy controls demonstrated robust dorsolateral prefrontal, anterior cingulate, and parietal cortex activity on both tasks. In contrast, patients with schizophrenia did not show any significant activation during proactive control, while showing activation similar to control subjects during reactive control. Critically, an interaction analysis showed that the degree to which prefrontal activity was reduced in patients versus controls depended on the type of control process engaged. Controls showed increased dorsolateral prefrontal cortex (DLPFC) and parietal activity in the proactive compared to the reactive control task, whereas patients with schizophrenia did not demonstrate this increase. Additionally, patients' DLPFC activity and performance during proactive control was associated with disorganization symptoms, while no reactive control measures showed this association. Proactive control processes and concomitant dysfunctional recruitment of DLPFC represent robust features of schizophrenia that are also directly associated with symptoms of disorganization

Structural Changes of Yellow Cameleon Domains Observed by Quantitative FRET Analysis and Polarized Fluorescence Correlation Spectroscopy

Förster resonance energy transfer (FRET) is a widely used method for monitoring interactions between or within biological macromolecules conjugated with suitable donor-acceptor pairs. Donor fluorescence lifetimes in absence and presence of acceptor molecules are often measured for the observation of FRET. However, these lifetimes may originate from interacting and noninteracting molecules, which hampers quantitative interpretation of FRET data. We describe a methodology for the detection of FRET that monitors the rise time of acceptor fluorescence on donor excitation thereby detecting only those molecules undergoing FRET. The large advantage of this method, as compared to donor fluorescence quenching method used more commonly, is that the transfer rate of FRET can be determined accurately even in cases where the FRET efficiencies approach 100% yielding highly quenched donor fluorescence. Subsequently, the relative orientation between donor and acceptor chromophores is obtained from time-dependent fluorescence anisotropy measurements carried out under identical conditions of donor excitation and acceptor detection. The FRET based calcium sensor Yellow Cameleon 3.60 (YC3.60) was used because it changes its conformation on calcium binding, thereby increasing the FRET efficiency. After mapping distances and orientation angles between the FRET moieties in YC3.60, cartoon models of this FRET sensor with and without calcium could be created. Independent support for these representations came from experiments where the hydrodynamic properties of YC3.60 under ensemble and single-molecule conditions on selective excitation of the acceptor were determined. From rotational diffusion times as found by fluorescence correlation spectroscopy and consistently by fluorescence anisotropy decay analysis it could be concluded that the open structure (without calcium) is flexible as opposed to the rather rigid closed conformation. The combination of two independent methods gives consistent results and presents a rapid and specific methodology to analyze structural and dynamical changes in a protein on ligand bindin