Turnover of BRCA1 Involves in Radiation-Induced Apoptosis

Background: Germ-line mutations of the breast cancer susceptibility gene-1 (BRCA1) increase the susceptibility to tumorigenesis. The function of BRCA1 is to regulate critical cellular processes, including cell cycle progression, genomic integrity, and apoptosis. Studies on the regulation of BRCA1 have focused intensely on transcription and phosphorylation mechanisms. Proteolytic regulation of BRCA1 in response to stress signaling remains largely unknown. The manuscript identified a novel mechanism by which BRCA1 is regulated by the ubiquitin-dependent degradation in response to ionization. Methodology/Principal Findings: Here, we report that severe ionization triggers rapid degradation of BRCA1, which in turn results in the activation of apoptosis. Ionization-induced BRCA1 turnover is mediated via an ubiquitin-proteasomal pathway. The stabilization of BRCA1 significantly delays the onset of ionization-induced apoptosis. We have mapped the essential region on BRCA1, which mediates its proteolysis in response to ionization. Moreover, we have demonstrated that BRCA1 protein is most sensitive to degradation when ionization occurs during G2/M and S phase. Conclusions/Significance: Our results suggest that ubiquitin-proteasome plays an important role in regulating BRCA1 during genotoxic stress. Proteolytic regulation of BRCA1 involves in ionization-induced apoptosis. © 2010 Liu et al

Influencing Factors and Theoretical Models for the Surface Topography in Diamond Turning Process: A Review

In this work, the influencing factors and corresponding theoretical models for the surface topography in diamond turning process are reviewed. The surface profile on one tool feed is the elementary unit of surface topography. The influences coupled with the models of the duplication effect of the tool edge profile, material spring back, and plastic side flow are outlined in this part. In light of the surface profile on one tool feed and “trim principle”, the modeling methods of surface topography along the radial direction (2D surface topography) are commented. Moreover, the influence of the vibration between the diamond tool and workpiece on the 2D surface topography is discussed, and the theoretical models are summarized. Finally, the issues for modeling of 3D surface topography, particularly the influences of material defects, are analyzed. According to the state-of-the-art surface topography model of the diamond turned component, future work in this field is therefore predicted

Experimental study on indoor environment quality in a naturally ventilated classroom of a university using natural ventilation and ventilation fan

Strengthening indoor ventilation is an important measure to improve air quality. In transitional season between spring and summer, a university classroom in Jinan city was selected as the research object. Mechanical air supply is adopted to solve the problem of insufficient fresh air or excessive carbon dioxide concentration in the classroom. CO2 concentration and air change rates are compared in natural ventilation and mechanical classrooms. The experiment shows that the indoor CO2 concentration of mechanical ventilation classroom is relatively low. Under natural ventilation, mechanical ventilation and mixed ventilation, the average air change rates were 1.05 h-1, 1.83 h-1 and 2.7 h-1, respectively. According to the statistics analysis of the questionnaire, 72.84% of the students hope to install the mechanical ventilation in the classroom

End Effect Analysis of a Slot-Less Long-Stator Permanent Magnet Linear Synchronous Motor

The implications of the end effect for flux linkage and thrust ripple in a slot-less long-stator permanent magnet linear synchronous motor (LSPMLSM), are analyzed in this paper. Since it is affected by the end effect, the air-gap magnetic field density under the end permanent magnet is different from that under the non-end permanent magnet, leading to asymmetry in the thrust ripple. For this reason, we establish a dynamic permanent magnet flux linkage model, which proves that the end effect leads to sub-harmonics in the permanent magnet flux linkage. The motor’s magnetic field distribution in the left and right parts is symmetrical. A thrust model taking into account the flux linkage sub-harmonics is established, from which the amplitude and period of the thrust ripple caused by the end effect can be calculated. There is no detent force for the slot-less LSPMLSM, and the end effect is the primary origin of the motor thrust ripple. In order to suppress the end effect, a method of increasing the end iron length is proposed, as a result of which the sub-harmonics in the flux linkage and the motor thrust ripple are effectively suppressed. Experimental and simulation results verify the results of this paper

Analysis of the Static Performance of a Cableless Aerostatic Guideway

To develop an innovative aerostatic guideway without cable drag force that can be widely applied to ultra-precision machining and measurement technology, it is necessary to analyze the static performance of the aerostatic guideway. The structural properties of the cableless aerostatic guideway, i.e., the bearing capacity and internal pressure distribution, directly affect the accuracy of processing and measurement. In this work, the relevant flow equations of the cableless aerostatic guideway are established by considering the unbalanced micro-scale air film. Moreover, the finite difference method is used to solve the calculation of the global analysis of air film at different positions. In addition, this work compares and analyzes the pressure and fluid velocity vector distributions in balanced and unbalanced states and investigates the effect of varying the thicknesses of the air film on static characteristics such as bearing capacity and stiffness. By comparing the balanced and unbalanced states under the same conditions, the obtained results show that in the unbalanced situation, the bearing capacity is lower by 11.69 percent and the stiffness is slightly higher (by 16.67 percent). Furthermore, the related experiments verify the predicted dependence of the bearing capacity on the thickness of the air film, and further demonstrate the feasibility of the proposed structure of the cableless aerostatic guideway. This work provides a technical reference for the design of a cableless aerostatic guideway

Analysis of the Static Performance of a Cableless Aerostatic Guideway

To develop an innovative aerostatic guideway without cable drag force that can be widely applied to ultra-precision machining and measurement technology, it is necessary to analyze the static performance of the aerostatic guideway. The structural properties of the cableless aerostatic guideway, i.e., the bearing capacity and internal pressure distribution, directly affect the accuracy of processing and measurement. In this work, the relevant flow equations of the cableless aerostatic guideway are established by considering the unbalanced micro-scale air film. Moreover, the finite difference method is used to solve the calculation of the global analysis of air film at different positions. In addition, this work compares and analyzes the pressure and fluid velocity vector distributions in balanced and unbalanced states and investigates the effect of varying the thicknesses of the air film on static characteristics such as bearing capacity and stiffness. By comparing the balanced and unbalanced states under the same conditions, the obtained results show that in the unbalanced situation, the bearing capacity is lower by 11.69 percent and the stiffness is slightly higher (by 16.67 percent). Furthermore, the related experiments verify the predicted dependence of the bearing capacity on the thickness of the air film, and further demonstrate the feasibility of the proposed structure of the cableless aerostatic guideway. This work provides a technical reference for the design of a cableless aerostatic guideway

Comprehensive Analysis of Network Robustness Evaluation Based on Convolutional Neural Networks with Spatial Pyramid Pooling

Connectivity robustness, a crucial aspect for understanding, optimizing, and repairing complex networks, has traditionally been evaluated through time-consuming and often impractical simulations. Fortunately, machine learning provides a new avenue for addressing this challenge. However, several key issues remain unresolved, including the performance in more general edge removal scenarios, capturing robustness through attack curves instead of directly training for robustness, scalability of predictive tasks, and transferability of predictive capabilities. In this paper, we address these challenges by designing a convolutional neural networks (CNN) model with spatial pyramid pooling networks (SPP-net), adapting existing evaluation metrics, redesigning the attack modes, introducing appropriate filtering rules, and incorporating the value of robustness as training data. The results demonstrate the thoroughness of the proposed CNN framework in addressing the challenges of high computational time across various network types, failure component types and failure scenarios. However, the performance of the proposed CNN model varies: for evaluation tasks that are consistent with the trained network type, the proposed CNN model consistently achieves accurate evaluations of both attack curves and robustness values across all removal scenarios. When the predicted network type differs from the trained network, the CNN model still demonstrates favorable performance in the scenario of random node failure, showcasing its scalability and performance transferability. Nevertheless, the performance falls short of expectations in other removal scenarios. This observed scenario-sensitivity in the evaluation of network features has been overlooked in previous studies and necessitates further attention and optimization. Lastly, we discuss important unresolved questions and further investigation.Comment: 25 pages, 8 figures, 7 tables, journa