Renal SDF-1 protein levels following LC treatment.

<p>Kidney homogenates from mice subjected to I/R injury and treated with LV or LC were analysed for SDF-1 protein using ELISA. LC treatment resulted in an increase of SDF-1 levels compared with the concentration found in homogenates from LV-treated animals that reached statistical significance (n  =  4-6 per group, **<i>P</i> <0.01). Animals were sacrificed 24 h following ischaemia.</p

SDF-1 protein levels after ischemia-reperfusion (I/R) injury.

<p>(A)Levels of SDF-1 were determined by ELISA using whole-kidney homogenates obtained from sham-operated animals or animals sacrificed at Day 1, 3 or 7 following ischaemia. The amount of SDF-1 at Day 1 was significantly higher compared with that found in sham-operated mice, and with still significantly elevated levels at day 3. Values are means ± SD; n = 6 per group. **<i>P</i> <0.01, vs sham; *<i>P</i> <0.05, vs sham. (B) SDF-1 mRNA levels after ischemia-reperfusion (I/R) injury. Real-time polymerase chain reaction (PCR) quantification of SDF-1 mRNA showed a significant increase at day 1 after ischemia. Values are means ± SD; n = 6 per group. *<i>P</i> <0.05, vs sham.</p

Kidney tissue injury over time following 30min of bilateral renal ischenmia-reperfusion injury.

<p>C57BL/6 mice were subjected to sham or bilateral ischemia by clamping the renal pedicles for 30 min and then removing the clamps and confirming reperfusion. Mice were sacrificed at various times and kidney samples were collected. (A and B) BUN and serum creatinine were measured to determine renal function.The data shown were the means±SD. n = 6 per group. *<i>P</i> <0.05, vs sham; **<i>P</i> <0.01, vs sham(C) Photomicrograps of H & E-stained kidney sections (200×). All fields were chosen form cortex and outer medulla. Tubular damage is marked with arrows.</p

Regional location of SDF-1 in I/R kidney. (A) Immunohistochemistry staining of SDF-1 in the kidney also showed that IR-induced expression of SDF-1 was further distributed into the surrounding corticomedullary and outer medullary region compared to sham-operated mice.

<p>The kidney sections from sham-operated mice were used as control. (upper panels original magnification 200×; bottom panels 400×). (B) Quantification of SDF-1 positive area. Values are means ± SD. **<i>P</i> <0.01, vs sham.</p

Tubular injury is attenuated in LC-treated mice (A) Histology of mice shows increased tubular injury in the LV+I/R group compared with LC+I/R. (200×).

<p>Tubular damage is marked with arrows.(B) Semiquantitative analysis of tubular damage in LC-treated and LV-treated kidney at 24h after reperfusion. Values are means ± SD; n = 6 per group. *<i>P</i> <0.05, vs I/R+LV. (C) Serum creatinine values are shown 24hours after I/R±macrophage infusion. **<i>P</i> <0.01, vs I/R+LV.</p

Proinflammatory macrophages accumulation following I/R.

<p>Photographs depicting macrophage in LV and LC treated mice exposed to sham operation or I/R. (arrows, 400×).</p

DataSheet_1_Analysis and prediction of marine heatwaves in the Western North Pacific and Chinese coastal region.pdf

Over the past decade, marine heatwaves (MHWs) research has been conducted in almost all of the world’s oceans, and their catastrophic effects on the marine environment have gradually been recognized. Using the second version of the Optimal Interpolated Sea Surface Temperature analysis data (OISSTV2) from 1982 to 2014, this study analyzes six MHWs characteristics in the Western North Pacific and Chinese Coastal region (WNPCC, 100°E ∼ 180°E, 0° ∼ 65°N). MHWs occur in most WNPCC areas, with an average frequency, duration, days, cumulative intensity, maximum intensity, and mean intensity of 1.95 ± 0.21 times/year, 11.38 ± 1.97 days, 22.06 ± 3.84 days, 18.06 ± 7.67 °Cdays, 1.84 ± 0.50°C, and 1.49 ± 0.42 °C, respectively, in the historical period (1982 ~ 2014). Comparing the historical simulation results of 19 models of the Coupled Model Intercomparison Project Phase 6 (CMIP6) with the OISSTV2 observations, five best-performing models (GFDL-CM4, GFDL-ESM4, AWI-CM-1-1-MR, EC-Earth3-Veg, and EC-Earth3) are selected for MHWs projection (2015 ~ 2100). The MHWs characteristics projections from these five models are analyzed in detail under the Shared Socio-economic Pathway (SSP) 1-2.6, 2-4.5 and 5-8.5 scenarios. The projected MHWs characteristics under SSP5-8.5 are more considerable than those under SSP1-2.6 and 2-4.5, except for the MHWs frequency. The MHWs cumulative intensity is 96.36 ± 56.30, 175.44 ± 92.62, and 385.22 ± 168.00 °Cdays under SSP1-2.6, 2-4.5 and 5-8.5 scenarios, respectively. This suggests that different emission scenarios have a crucial impact on MHW variations. Each MHWs characteristic has an obvious increasing trend except for the annual occurrences. The increase rate of MHWs cumulative intensity for these three scenarios is 1.02 ± 0.83, 3.83 ± 1.43, and 6.70 ± 2.61 °Cdays/year, respectively. The MHWs occurrence area in summer is slightly smaller than in winter, but the MHWs average intensity is stronger in summer than in winter.</p